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Protein

Hydroperoxide isomerase ALOXE3

Gene

Aloxe3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced dioxygenase activity compared to other lipoxygenases. The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols. The dioxygenase activity requires a step of activation of the enzyme by molecular oxygen. In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides. In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites. Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activates PPARG. Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia.1 Publication

Catalytic activityi

A hydroperoxy icosatetraenoate = a hydroxy epoxy icosatrienoate.By similarity
A hydroperoxy icosatetraenoate = an oxoicosatetraenoate + H2O.By similarity

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi408Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi413Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi588Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi592Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi711Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Isomerase, Lyase, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
UniPathwayiUPA00222
UPA00881

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroperoxide isomerase ALOXE3 (EC:5.4.4.7By similarity)
Alternative name(s):
Epidermis-type lipoxygenase 3
Short name:
Epidermal LOX-3
Short name:
e-LOX-3
Short name:
eLOX-3
Hydroperoxy icosatetraenoate dehydratase (EC:4.2.1.152By similarity)
Gene namesi
Name:Aloxe3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1306252 Aloxe3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004234191 – 711Hydroperoxide isomerase ALOXE3Add BLAST711

Proteomic databases

PaxDbiD3ZKX9

Expressioni

Gene expression databases

BgeeiENSRNOG00000007454
GenevisibleiD3ZKX9 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010141

Structurei

3D structure databases

SMRiD3ZKX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119PLATPROSITE-ProRule annotationAdd BLAST118
Domaini119 – 711LipoxygenasePROSITE-ProRule annotationAdd BLAST593

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
InParanoidiD3ZKX9
KOiK18684
OMAiPVHCLNG
OrthoDBiEOG091G04A4
PhylomeDBiD3ZKX9
TreeFamiTF105320

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

D3ZKX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVYRLCVTT GSYLKAGTLD NIYATLVGTC GESPKQKLDR VGRDFATGSV
60 70 80 90 100
QKYKVRCASE LGEILLLRLH KERFAFFCKD PWYCSRICVT TPDGSVVHFP
110 120 130 140 150
CYQWIDGYCT VELRPGTART ICQDALPLLL DHRKRELQAR QECYRWKIYA
160 170 180 190 200
PGFPRMVDVS SFEEMESDKK FALTKTAPCA DQDDNSGNRY LPGFPMKVDI
210 220 230 240 250
PSLLHMEPNI RYSATKTASL IFNALPASLG MKIRGLLDRK GSWKRLDDIR
260 270 280 290 300
NIFWCHKTFT SEYVTEHWCE DSFFGYQYLN GVNPIMLHCL SSLPSKLPVT
310 320 330 340 350
NDMVAPLLGP GTCLQTELER GHIFLADYWI LAEAPVHCLN GRQQYVTAPL
360 370 380 390 400
CLLWLNPQGV LLPLAIQLSQ IPGPESPIFL PTDCELDWLL AKTWVRNSEF
410 420 430 440 450
LVHENNTHFL CTHLLCEAFS MATLRQLPLC HPVYKLLLPH TRYTLQVNTI
460 470 480 490 500
ARATLLNPDG LVDKVTSIGR RGLIYLMSTG LAHFTYTDFC LPDSLRARGV
510 520 530 540 550
LTIPNYHYRD DGLKIWAAIE RFVSEIVSYY YPNDACVQQD SELQAWVGEI
560 570 580 590 600
FAQAFLGRES SGFPSRLCTP GELVKYLTAI IFNCSAQHAA VNSGQHDFGA
610 620 630 640 650
WMPNAPSSMR QPPPQTKGNT TMESYLETLP EVNTTCSNLL LFWLVSQEPK
660 670 680 690 700
DQRPLGTYPD EHFTEEAPRQ SIAAFQKCLA QISKDIRARN ESLALPYAYL
710
DPPLIENSVS I
Length:711
Mass (Da):80,440
Last modified:April 20, 2010 - v1
Checksum:i41C3D9BBC6C4532B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06064371 Genomic DNA No translation available.
CH473948 Genomic DNA Translation: EDM04845.1
RefSeqiNP_001099263.1, NM_001105793.1
UniGeneiRn.8716

Genome annotation databases

EnsembliENSRNOT00000010141; ENSRNOP00000010141; ENSRNOG00000007454
GeneIDi287424
KEGGirno:287424
UCSCiRGD:1306252 rat

Similar proteinsi

Entry informationi

Entry nameiLOXE3_RAT
AccessioniPrimary (citable) accession number: D3ZKX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: April 20, 2010
Last modified: March 28, 2018
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health