D3ZKB9 (TSH3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Teashirt homolog 3

Alternative name(s):
Zinc finger protein 537

Gene names

Name:	Tshz3
Synonyms:	Zfp537

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1072 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional regulator involved in developmental processes. Function in association with APBB1, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. TSHZ3-mediated transcription repression involves the recruitment of histone deacetylases HDAC1 and HDAC2. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s). Regulates the development of neurons involved in both respiratory rhythm and airflow control. Promotes maintenance of nucleus ambiguus (nA) motoneurons, which govern upper airway function, and establishes a respiratory rhythm generator (RRG) activity compatible with survival at birth. Involved in the differentiation of the proximal uretic smooth muscle cells during developmental processes. Involved in the up-regulation of myocardin, that directs the expression of smooth muscle cells in the proximal ureter By similarity.
Subunit structure	Interacts (via N-terminus) with HDAC1 and HDAC2; the interaction is direct. Found in a trimeric complex with APBB1 and HDAC1; the interaction between HDAC1 and APBB1 is mediated by TSHZ3 By similarity. Interacts (via homeobox domain) with APBB1 (via PID domain 1). Ref.2
Subcellular location	Nucleus. Cell projection › growth cone. Note: Colocalizes with APBB1 in the nucleus By similarity. Colocalizes with APBB1 in axonal growth cone. Ref.2
Tissue specificity	Expressed in cortical neurons. Ref.2
Sequence similarities	Belongs to the teashirt C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. Contains 1 homeobox DNA-binding domain.
Sequence caution	The sequence EDM07600.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cell projection Nucleus
Domain	Homeobox Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Developmental protein Repressor
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	in utero embryonic development Inferred from electronic annotation. Source: Compara kidney morphogenesis Inferred from electronic annotation. Source: Compara kidney smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara metanephros development Inferred from electronic annotation. Source: Compara musculoskeletal movement Inferred from electronic annotation. Source: Compara negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara regulation of respiratory gaseous exchange by neurological system process Inferred from sequence or structural similarity. Source: UniProtKB sensory perception of touch Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ureter smooth muscle cell differentiation Inferred from electronic annotation. Source: Compara ureteric bud development Inferred from electronic annotation. Source: Compara ureteric peristalsis Inferred from electronic annotation. Source: Compara
Cellular_component	growth cone Inferred from direct assay Ref.2. Source: UniProtKB mitochondrion Inferred from electronic annotation. Source: Compara nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1072	1072	Teashirt homolog 3		PRO_0000399476
Regions
	Zinc finger	204 – 228	25	C2H2-type 1
	Zinc finger	265 – 289	25	C2H2-type 2
	Zinc finger	376 – 400	25	C2H2-type 3; atypical
	DNA binding	882 – 952	71	Homeobox; atypical
	Zinc finger	967 – 989	23	C2H2-type 4
	Zinc finger	1032 – 1055	24	C2H2-type 5
	Compositional bias	131 – 154	24	Ser-rich
Amino acid modifications
	Modified residue	672	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	D3ZKB9 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: 9CB7824517771F35	FASTA	1,072	117,463
	10 20 30 40 50 60 MAAYVSDELK AAALVEDDIE PEEQVADGEP SAKYMCPEKE LSKACPSYQN SPAAEFSSHE 70 80 90 100 110 120 MDSESHISET SDRMADFESS SIKNEEETKE VQVPLEDTTV SDSLEQMKAV YNNFLSNSYW 130 140 150 160 170 180 SNLNLNLHQP SSENNGGGSS SSSSSSSSSC GSGSFDWHQS AMAKTLQQVS QNRMLPEPSL 190 200 210 220 230 240 FSTVQLYRQS SKLYGSIFTG ASKFRCKDCS AAYDTLVELT VHMNETGHYR DDNHETDNNN 250 260 270 280 290 300 PKRWSKPRKR SLLEMEGKED AQKVLKCMYC GHSFESLQDL SVHMIKTKHY QKVPLKEPVT 310 320 330 340 350 360 PVAAKIIPAA RKKPSLELEL PSSPDSTGGT PKATLSDASD ALQKNSNPYI TPNNRYGHQN 370 380 390 400 410 420 GASYAWHFEA RKSQILKCME CGSSHDTLQE LTAHMMVTGH FIKVTNSAMK KGKPIMETPV 430 440 450 460 470 480 TPTITTLLDE KVQSVPLAAT TFTSPSNTPA SVSPKLTVEI KKEVDKEKAV LDEKPKEKEK 490 500 510 520 530 540 ASEEEEKYDI SSKYHYLTEN DLEESPKGGL DILKSLENTV TSAINKAQNG TPSWGGYPSI 550 560 570 580 590 600 HAAYQLPNMM KLSLGSSGKS TPLKPMFGNS EIVSPTKTQT LVSPPSSQTS PMPKTNFHAM 610 620 630 640 650 660 EELVKKVTEK VAKVEEKMKE PDSKLSPPKR ATPSPCSSEQ SEPIKMEASS GSGFKSQENS 670 680 690 700 710 720 PSPPRDVCKE ASPSAEPVEN GKELVKPLSG GSLSGSTAII TDHPPEQPFV NPLSALQSVM 730 740 750 760 770 780 NIHLGKAAKP SLPALDPMSM LFKMSNSLAE KAAVATPPPL QAKKAEHLDR YFYHVNNDQP 790 800 810 820 830 840 IDLTKGKSDK GCSLGSGLLS PTSTSPATSS STVTTAKTSA VVSFMSNSPL RENALSDISD 850 860 870 880 890 900 MLKNLTESHT SKSSTPSSIS EKSDIDGATL EEAEESTPAQ KRKGRQSNWN PQHLLILQAQ 910 920 930 940 950 960 FAASLRQTSE GKYIMSDLSP QERMHISRFT GLSMTTISHW LANVKYQLRR TGGTKFLKNL 970 980 990 1000 1010 1020 DTGHPVFFCN DCASQIRTPS TYISHLESHL GFRLRDLSKL STEQINNQIA QTKSPSEKMV 1030 1040 1050 1060 1070 TSSPEEDLGT TYQCKLCNRT FASKHAVKLH LSKTHGKSPE DHLLFVSELE KQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"FE65 binds Teashirt, inhibiting expression of the primate-specific caspase-4." Kajiwara Y., Akram A., Katsel P., Haroutunian V., Schmeidler J., Beecham G., Haines J.L., Pericak-Vance M.A., Buxbaum J.D. PLoS ONE 4:E5071-E5071(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH APBB1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Web resources

Protein Spotlight

Life's first breath - Issue 122 of October 2010

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473979 Genomic DNA. Translation: EDM07600.1. Different initiation.
IPI	IPI00367252.
RefSeq	NP_001100976.1. NM_001107506.1.
UniGene	Rn.9411.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000018931; ENSRNOP00000018931; ENSRNOG00000013938.
GeneID	308523.
KEGG	rno:308523.
UCSC	RGD:1306322. rat.
Organism-specific databases
CTD	57616.
RGD	1306322. Tshz3.
Phylogenomic databases
GeneTree	ENSGT00390000014977.
KO	K09236.
OMA	DPMSMLF.
OrthoDB	EOG48GW2H.
Family and domain databases
InterPro	IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR027008. Teashirt_fam. IPR026810. Tshz3. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
PANTHER	PTHR12487. PTHR12487. 1 hit. PTHR12487:SF5. PTHR12487:SF5. 1 hit.
Pfam	PF00096. zf-C2H2. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. SM00355. ZnF_C2H2. 5 hits. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. False negative. PS50071. HOMEOBOX_2. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 4 hits. PS50157. ZINC_FINGER_C2H2_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	659069.

Entry information

Entry name

TSH3_RAT

Accession

Primary (citable) accession number: D3ZKB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program