D3ZHS6 (BAP1_RAT) Reviewed, UniProtKB/Swiss-Prot
Last modified
March 6, 2013.
Version 17.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase BAP1 EC=3.4.19.12 Alternative name(s): BRCA1-associated protein 1 | ||
| Gene names |
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| Organism | Rattus norvegicus (Rat) [Reference proteome] | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 727 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1 By similarity. |
| Subcellular location | Cytoplasm By similarity. Nucleus By similarity. Note: Mainly nuclear. Binds to chromatin By similarity. |
| Sequence similarities | Belongs to the peptidase C12 family. BAP1 subfamily. |
| Sequence caution | The sequence EDL88955.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 727 | 727 | Ubiquitin carboxyl-terminal hydrolase BAP1 | PRO_0000395817 | |||||
Regions | |||||||||
| Region | 594 – 719 | 126 | Interaction with BRCA1 By similarity | ||||||
| Coiled coil | 628 – 659 | 32 | Potential | ||||||
| Motif | 363 – 366 | 4 | HBM-like motif By similarity | ||||||
| Motif | 715 – 720 | 6 | Nuclear localization signal By similarity | ||||||
Sites | |||||||||
| Active site | 91 | 1 | Nucleophile By similarity | ||||||
| Active site | 169 | 1 | Proton donor By similarity | ||||||
| Site | 184 | 1 | Important for enzyme activity By similarity | ||||||
Sequences
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References
| [1] | Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CH474046 Genomic DNA. Translation: EDL88955.1. Sequence problems. |
| IPI | IPI00870786. |
| RefSeq | NP_001100762.1. NM_001107292.1. |
| UniGene | Rn.3382. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| MEROPS | C12.004. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOT00000025853; ENSRNOP00000025853; ENSRNOG00000019097. |
| GeneID | 306257. |
| KEGG | rno:306257. |
| UCSC | RGD:1311938. rat. |
Organism-specific databases | |
| CTD | 8314. |
| RGD | 1311938. Bap1. |
Phylogenomic databases | |
| GeneTree | ENSGT00510000046560. |
| KO | K08588. |
| OMA | PQQYSDD. |
Family and domain databases | |
| Gene3D | 3.40.532.10. 1 hit. |
| InterPro | IPR001578. Peptidase_C12. [Graphical view] |
| PANTHER | PTHR10589. PTHR10589. 1 hit. |
| Pfam | PF01088. Peptidase_C12. 1 hit. [Graphical view] |
| PRINTS | PR00707. UBCTHYDRLASE. |
| PROSITE | PS00140. UCH_1. False negative. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 655725. |
Entry information
| Entry name | BAP1_RAT | ||||||||
| Accession | Primary (citable) accession number: D3ZHS6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |