D3ZE55 (PCDH8_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protocadherin-8

Alternative name(s):
Activity-regulated cadherin-like protein
Short name=Arcadlin

Gene names

Name:

Pcdh8

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1069 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-dependent cell-adhesion protein. May play a role in activity-induced synaptic reorganization underlying long term memory. Could be involved in CDH2 internalization through TAOK2/p38 MAPK pathway. In hippocampal neurons, may play a role in the down-regulation of dendritic spines, maybe through its action on CDH2 endocytosis. Ref.1 Ref.4
Subunit structure	The N-terminal extracellular domain forms homophilic interactions; these interactions activate p38 MAPK via TAOK2 and trigger endocytosis. Interacts with CDH2; this interaction may lead to CDH2 cointernalization. Interacts with CDH11. Interacts with TAOK2. Ref.1 Ref.4
Subcellular location	Cell membrane; Single-pass type I membrane protein By similarity. Cell projection › dendrite. Cell junction › synapse › presynaptic cell membrane. Cell junction › synapse › postsynaptic cell membrane. Note: Also expressed in the cell bodies of neurons of the hippocampus and cortex. Localized to excitatory, but not with inhibitory, synapses. Ref.1
Tissue specificity	Enriched in brain relative to peripheral tissues, with low expression in the testis. Expressed in hippocampal neurons (at protein level). Ref.1
Developmental stage	At E17, expressed in the auditory circuit, most prominently in the inferior colliculus, and later in the medial geniculate and the auditory cortex at P0. At P0, also expressed in targets of retinal projections, such as the superior colliculus, the suprachiasmatic nucleus, and the ventrolateral geniculate nucleus. At the same stage, detected in selected structures of the limbic circuit, including the anterior limbic thalamic nuclei, the hippocampus, amygdala and habenula. Ref.1
Induction	Rapidly and transiently induced by maximal electroconvulsive seizure in the hippocampal granule cells, and modestly induced in the pyramidal cells. Also induced by cAMP. Ref.1 Ref.4
Sequence similarities	Contains 6 cadherin domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Cell junction Cell membrane Cell projection Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	homophilic cell adhesion Inferred from electronic annotation. Source: InterPro long-term memory Traceable author statement Ref.1. Source: RGD morphogenesis of embryonic epithelium Inferred from electronic annotation. Source: Compara somitogenesis Inferred from electronic annotation. Source: Compara synaptic transmission Inferred from direct assay Ref.1. Source: RGD
Cellular_component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW dendrite Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: D3ZE55-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: D3ZE55-2)

The sequence of this isoform differs from the canonical sequence as follows:
     779-875: Missing.

Note: May be the only physiologically relevant isoform (PubMed:10383464).

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 1069	1040	Protocadherin-8		PRO_0000404297
Regions
	Topological domain	30 – 747	718	Extracellular Potential
	Transmembrane	748 – 768	21	Helical; Potential
	Topological domain	769 – 1069	301	Cytoplasmic Potential
	Domain	30 – 135	106	Cadherin 1
	Domain	136 – 245	110	Cadherin 2
	Domain	247 – 354	108	Cadherin 3
	Domain	393 – 497	105	Cadherin 4
	Domain	498 – 609	112	Cadherin 5
	Domain	615 – 721	107	Cadherin 6
Amino acid modifications
	Glycosylation	616	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	779 – 875	97	Missing in isoform 2.		VSP_040565
Experimental info
	Sequence conflict	351	1	A → P in BAA82442. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: 307DB9763F69D7D3	FASTA	1,069	113,191
	10 20 30 40 50 60 MSPVKRWGSP CLFPLQLFSL CWVLSVAQSK TVRYSTFEED APGTVIGTLA EDLHMKVSGD 70 80 90 100 110 120 TSFRLMKQFN SSLLRVREGD GQLTVGDAGL DRERLCGQSP QCVLAFDVVS FSQEQFRLVH 130 140 150 160 170 180 VEVEVRDVND HAPRFPRAQI PVEVSESAPV GTRIPLEVPV DEDVGANGLQ SVRLAEPHSP 190 200 210 220 230 240 FRVELQTRAD GAQCADLVLL QELDRESQAS YSLELVAQDG GRPPRSATAA LSVRVLDAND 250 260 270 280 290 300 HSPAFPQGAV AEVELAEDAP VGSLLLDLDA ADPDEGPNGD VVFTFGARTP PEARHLFRLD 310 320 330 340 350 360 PRSGRLTLAG QVDYERQDTY ELDVRAQDRG PGPRTATCKV IVRIRDVNDN APDISITPLA 370 380 390 400 410 420 APGAPATSPF AAAAAAAALG GADAASSAGS GTQETGVTSL VPEGAARESL VALVSTSDRD 430 440 450 460 470 480 SGANGQVRCA LYGHEHFRLQ PAYAGSYLVV TAASLDRERI AEYNLTLVAE DRGAPPLRTV 490 500 510 520 530 540 RPYTVRVGDE NDNAPLFTKP VYEVSVRENN PPGAYLATVA ARDPDLGRNG QVTYRLVEAE 550 560 570 580 590 600 VGRSGEAVST YVSVDPATGA IYALRSFDYE TLRQLDVRVQ ASDGGSPQLS SNALVQVRVL 610 620 630 640 650 660 DQNDHSPVLV HPAPANGSLE VAVPGRSTKD TAVARIQARD ADEGANGELA FDLLQQEPRE 670 680 690 700 710 720 AFSIGRHTGE IVLTGDLSQE PPGRVFKALL VISDGGRPPL TTTATVSFVV TAGGGSAVPA 730 740 750 760 770 780 SAGSPEHFRP PGSRLAPSGP SLQWDTPLIV IIVLAGSCTL LLAAIIAIAT TCNRRKKEVR 790 800 810 820 830 840 KGGALREERP GAAGGGASAP GSPDETARGT GPRPNMFDVL TFPGSGKAPF GSPAADAPPP 850 860 870 880 890 900 AVAAAEVPGS EGGSATGESA CHFEGQQRLR GAHAEPYSAS PGFGKEPAPP VAVWKGHSFN 910 920 930 940 950 960 TISGREAEKF SGKDSGKGDS DFNDSDSDIS GDALKKDLIN HMQSGLWACT AECKILGHSD 970 980 990 1000 1010 1020 RCWSPSCAGP NTHPPPHPPA QMSTFCKSTS LPRDPLRRDN YYQAQLPKTV GLQSVYEKVL 1030 1040 1050 1060 HRDYDRTVTL LSPPRPGRLP DLQEIGVPLY ESPPGGRYVS PKKGTNENV
	Isoform 2 [UniParc]. Checksum: 6968FA65FF82123F	FASTA	972	103,774
	10 20 30 40 50 60 MSPVKRWGSP CLFPLQLFSL CWVLSVAQSK TVRYSTFEED APGTVIGTLA EDLHMKVSGD 70 80 90 100 110 120 TSFRLMKQFN SSLLRVREGD GQLTVGDAGL DRERLCGQSP QCVLAFDVVS FSQEQFRLVH 130 140 150 160 170 180 VEVEVRDVND HAPRFPRAQI PVEVSESAPV GTRIPLEVPV DEDVGANGLQ SVRLAEPHSP 190 200 210 220 230 240 FRVELQTRAD GAQCADLVLL QELDRESQAS YSLELVAQDG GRPPRSATAA LSVRVLDAND 250 260 270 280 290 300 HSPAFPQGAV AEVELAEDAP VGSLLLDLDA ADPDEGPNGD VVFTFGARTP PEARHLFRLD 310 320 330 340 350 360 PRSGRLTLAG QVDYERQDTY ELDVRAQDRG PGPRTATCKV IVRIRDVNDN APDISITPLA 370 380 390 400 410 420 APGAPATSPF AAAAAAAALG GADAASSAGS GTQETGVTSL VPEGAARESL VALVSTSDRD 430 440 450 460 470 480 SGANGQVRCA LYGHEHFRLQ PAYAGSYLVV TAASLDRERI AEYNLTLVAE DRGAPPLRTV 490 500 510 520 530 540 RPYTVRVGDE NDNAPLFTKP VYEVSVRENN PPGAYLATVA ARDPDLGRNG QVTYRLVEAE 550 560 570 580 590 600 VGRSGEAVST YVSVDPATGA IYALRSFDYE TLRQLDVRVQ ASDGGSPQLS SNALVQVRVL 610 620 630 640 650 660 DQNDHSPVLV HPAPANGSLE VAVPGRSTKD TAVARIQARD ADEGANGELA FDLLQQEPRE 670 680 690 700 710 720 AFSIGRHTGE IVLTGDLSQE PPGRVFKALL VISDGGRPPL TTTATVSFVV TAGGGSAVPA 730 740 750 760 770 780 SAGSPEHFRP PGSRLAPSGP SLQWDTPLIV IIVLAGSCTL LLAAIIAIAT TCNRRKKEPY 790 800 810 820 830 840 SASPGFGKEP APPVAVWKGH SFNTISGREA EKFSGKDSGK GDSDFNDSDS DISGDALKKD 850 860 870 880 890 900 LINHMQSGLW ACTAECKILG HSDRCWSPSC AGPNTHPPPH PPAQMSTFCK STSLPRDPLR 910 920 930 940 950 960 RDNYYQAQLP KTVGLQSVYE KVLHRDYDRT VTLLSPPRPG RLPDLQEIGV PLYESPPGGR 970 YVSPKKGTNE NV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arcadlin is a neural activity-regulated cadherin involved in long term potentiation." Yamagata K., Andreasson K.I., Sugiura H., Maru E., Dominique M., Irie Y., Miki N., Hayashi Y., Yoshioka M., Kaneko K., Kato H., Worley P.F. J. Biol. Chem. 274:19473-19479(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Tissue: Hippocampus.
[2]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-694. Strain: Brown Norway/NHsdMcwi. Tissue: Embryonic brain.
[4]	"Activity-induced protocadherin arcadlin regulates dendritic spine number by triggering N-cadherin endocytosis via TAO2beta and p38 MAP kinases." Yasuda S., Tanaka H., Sugiura H., Okamura K., Sakaguchi T., Tran U., Takemiya T., Mizoguchi A., Yagita Y., Sakurai T., De Robertis E.M., Yamagata K. Neuron 56:456-471(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDH2; CDH11 AND TAOK2, INDUCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026154 mRNA. Translation: BAA82442.1. CH473951 Genomic DNA. Translation: EDM02373.1. BC128737 mRNA. Translation: AAI28738.1.
IPI	IPI00205290. IPI00975253.
RefSeq	NP_074059.1. NM_022868.1.
UniGene	Rn.23337.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017599.
PTM databases
PhosphoSite	D3ZE55.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017599; ENSRNOP00000017599; ENSRNOG00000013101.
GeneID	64865.
KEGG	rno:64865.
Organism-specific databases
CTD	5100.
RGD	69350. Pcdh8.
Phylogenomic databases
GeneTree	ENSGT00690000101998.
HOVERGEN	HBG054878.
KO	K16499.
OrthoDB	EOG4FJ8B5.
Family and domain databases
Gene3D	2.60.40.60. 6 hits.
InterPro	IPR002126. Cadherin. IPR015919. Cadherin-like. IPR020894. Cadherin_CS. IPR013164. Cadherin_N. [Graphical view]
Pfam	PF00028. Cadherin. 5 hits. PF08266. Cadherin_2. 1 hit. [Graphical view]
PRINTS	PR00205. CADHERIN.
SMART	SM00112. CA. 6 hits. [Graphical view]
SUPFAM	SSF49313. Cadherin. 6 hits.
PROSITE	PS00232. CADHERIN_1. 5 hits. PS50268. CADHERIN_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	613776.

Entry information

Entry name

PCDH8_RAT

Accession

Primary (citable) accession number: D3ZE55
Secondary accession number(s): A1A5N4, Q9WVR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	February 8, 2011
Last modified:	April 3, 2013
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program