Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin-conjugating enzyme E2 D1

Gene

Ube2d1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4 (By similarity). Mediates ubiquitination of PEX5.By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationBy similarity
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)-monoubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-RNO-176412. Phosphorylation of the APC/C.
R-RNO-179409. APC-Cdc20 mediated degradation of Nek2A.
R-RNO-201451. Signaling by BMP.
R-RNO-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-RNO-5689896. Ovarian tumor domain proteases.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 D1 (EC:2.3.2.23)
Alternative name(s):
(E3-independent) E2 ubiquitin-conjugating enzyme D1 (EC:2.3.2.24)
E2 ubiquitin-conjugating enzyme D1
Ubiquitin carrier protein D1
Ubiquitin-conjugating enzyme E2(17)KB 1
Ubiquitin-conjugating enzyme E2-17 kDa 1
Ubiquitin-protein ligase D1
Gene namesi
Name:Ube2d1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1307886. Ube2d1.

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003960061 – 147Ubiquitin-conjugating enzyme E2 D1Add BLAST147

Proteomic databases

PeptideAtlasiD3ZDK2.

Expressioni

Gene expression databases

ExpressionAtlasiD3ZDK2. baseline and differential.
GenevisibleiD3ZDK2. RN.

Interactioni

Subunit structurei

Component of a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts with RNF11.By similarity

Protein-protein interaction databases

BioGridi262993. 1 interactor.

Structurei

3D structure databases

ProteinModelPortaliD3ZDK2.
SMRiD3ZDK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000119012.
InParanoidiD3ZDK2.
KOiK06689.
OMAiQEIAREW.
OrthoDBiEOG091G0GF8.
PhylomeDBiD3ZDK2.
TreeFamiTF101108.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3ZDK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKRIQKEL SDLQRDPPAH CSAGPVGDDL FHWQATIMGP PDSAYQGGVF
60 70 80 90 100
FLTVHFPTDY PFKPPKIAFT TKIYHPNINS NGSICLDILR SQWSPALTVS
110 120 130 140
KVLLSICSLL CDPNPDDPLV PDIAQIYKSD KEKYNRHARE WTQKYAM
Length:147
Mass (Da):16,602
Last modified:April 20, 2010 - v1
Checksum:i2E96FD0179EE119D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473988 Genomic DNA. Translation: EDL97256.1.
RefSeqiNP_001102000.1. NM_001108530.1.
UniGeneiRn.24937.

Genome annotation databases

EnsembliENSRNOT00000000750; ENSRNOP00000000750; ENSRNOG00000000611.
GeneIDi361831.
KEGGirno:361831.
UCSCiRGD:1307886. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473988 Genomic DNA. Translation: EDL97256.1.
RefSeqiNP_001102000.1. NM_001108530.1.
UniGeneiRn.24937.

3D structure databases

ProteinModelPortaliD3ZDK2.
SMRiD3ZDK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi262993. 1 interactor.

Proteomic databases

PeptideAtlasiD3ZDK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000750; ENSRNOP00000000750; ENSRNOG00000000611.
GeneIDi361831.
KEGGirno:361831.
UCSCiRGD:1307886. rat.

Organism-specific databases

CTDi7321.
RGDi1307886. Ube2d1.

Phylogenomic databases

GeneTreeiENSGT00760000119012.
InParanoidiD3ZDK2.
KOiK06689.
OMAiQEIAREW.
OrthoDBiEOG091G0GF8.
PhylomeDBiD3ZDK2.
TreeFamiTF101108.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-RNO-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-RNO-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-RNO-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-RNO-176412. Phosphorylation of the APC/C.
R-RNO-179409. APC-Cdc20 mediated degradation of Nek2A.
R-RNO-201451. Signaling by BMP.
R-RNO-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-RNO-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

PROiD3ZDK2.

Gene expression databases

ExpressionAtlasiD3ZDK2. baseline and differential.
GenevisibleiD3ZDK2. RN.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUB2D1_RAT
AccessioniPrimary (citable) accession number: D3ZDK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: April 20, 2010
Last modified: November 30, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.