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Protein

E3 ubiquitin-protein ligase HACE1

Gene

Hace1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei876Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processCell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HACE1 (EC:2.3.2.26)
Alternative name(s):
HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
HECT-type E3 ubiquitin transferase HACE1
Gene namesi
Name:Hace1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1306114 Hace1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004158431 – 909E3 ubiquitin-protein ligase HACE1Add BLAST909

Proteomic databases

PaxDbiD3ZBM7
PeptideAtlasiD3ZBM7
PRIDEiD3ZBM7

Expressioni

Gene expression databases

BgeeiENSRNOG00000000327
GenevisibleiD3ZBM7 RN

Interactioni

Subunit structurei

Interacts with RARB. Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000365

Structurei

3D structure databases

ProteinModelPortaliD3ZBM7
SMRiD3ZBM7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati64 – 93ANK 1Add BLAST30
Repeati97 – 126ANK 2Add BLAST30
Repeati130 – 159ANK 3Add BLAST30
Repeati163 – 192ANK 4Add BLAST30
Repeati196 – 226ANK 5Add BLAST31
Repeati228 – 257ANK 6Add BLAST30
Domaini574 – 909HECTPROSITE-ProRule annotationAdd BLAST336

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0939 Eukaryota
KOG4177 Eukaryota
COG0666 LUCA
COG5021 LUCA
GeneTreeiENSGT00760000118966
InParanoidiD3ZBM7
KOiK12166
OMAiNDMGYNG
OrthoDBiEOG091G025R
TreeFamiTF323417

Family and domain databases

CDDicd00204 ANK, 1 hit
cd00078 HECTc, 1 hit
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
PfamiView protein in Pfam
PF12796 Ank_2, 3 hits
PF00632 HECT, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SM00119 HECTc, 1 hit
SUPFAMiSSF48403 SSF48403, 1 hit
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
PS50237 HECT, 1 hit

Sequencei

Sequence statusi: Complete.

D3ZBM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL
60 70 80 90 100
SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT
110 120 130 140 150
PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL
160 170 180 190 200
VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVAGATP
210 220 230 240 250
LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ
260 270 280 290 300
YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT
310 320 330 340 350
TNGHKLLSLS SSYEAQMKSL LRIVRIFCHV FRIGPSSPSN GMDMGYNGNK
360 370 380 390 400
TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSADITS ILLKQKGQDQ
410 420 430 440 450
EASCISAFEP PGPGSYERLS TGPGESKPDV LAGKQEASAD CQDVISVTAN
460 470 480 490 500
RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD
510 520 530 540 550
HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS
560 570 580 590 600
ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
610 620 630 640 650
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL
660 670 680 690 700
ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS
710 720 730 740 750
DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT
760 770 780 790 800
RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT
810 820 830 840 850
EYTSGYERED PVIQWFWEVV EDMTQEERVL LLQFVTGSSR VPHGGFANIM
860 870 880 890 900
GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH

CGSYGYTMA
Length:909
Mass (Da):102,159
Last modified:April 20, 2010 - v1
Checksum:i60C1FC2E282C2F99
GO

Sequence databases

RefSeqiNP_001102009.2, NM_001108539.3
UniGeneiRn.28116

Genome annotation databases

EnsembliENSRNOT00000000365; ENSRNOP00000000365; ENSRNOG00000000327
GeneIDi361866
KEGGirno:361866
UCSCiRGD:1306114 rat

Similar proteinsi

Entry informationi

Entry nameiHACE1_RAT
AccessioniPrimary (citable) accession number: D3ZBM7
Secondary accession number(s): D3ZBM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: April 20, 2010
Last modified: April 25, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health