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Protein

E3 ubiquitin-protein ligase HACE1

Gene

Hace1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei876 – 8761Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HACE1 (EC:6.3.2.-)
Alternative name(s):
HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
Gene namesi
Name:Hace1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi1306114. Hace1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 909909E3 ubiquitin-protein ligase HACE1PRO_0000415843Add
BLAST

Proteomic databases

PaxDbiD3ZBM7.
PeptideAtlasiD3ZBM7.

Expressioni

Gene expression databases

BgeeiENSRNOG00000000327.
GenevisibleiD3ZBM7. RN.

Interactioni

Subunit structurei

Interacts with RARB. Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000365.

Structurei

3D structure databases

ProteinModelPortaliD3ZBM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati64 – 9330ANK 1Add
BLAST
Repeati97 – 12630ANK 2Add
BLAST
Repeati130 – 15930ANK 3Add
BLAST
Repeati163 – 19230ANK 4Add
BLAST
Repeati196 – 22631ANK 5Add
BLAST
Repeati228 – 25730ANK 6Add
BLAST
Domaini574 – 909336HECTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
KOG4177. Eukaryota.
COG0666. LUCA.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
InParanoidiD3ZBM7.
KOiK12166.
OMAiTCEILIQ.
OrthoDBiEOG091G025R.
TreeFamiTF323417.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 3 hits.
PF00632. HECT. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50237. HECT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3ZBM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL
60 70 80 90 100
SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT
110 120 130 140 150
PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL
160 170 180 190 200
VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVAGATP
210 220 230 240 250
LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ
260 270 280 290 300
YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT
310 320 330 340 350
TNGHKLLSLS SSYEAQMKSL LRIVRIFCHV FRIGPSSPSN GMDMGYNGNK
360 370 380 390 400
TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSADITS ILLKQKGQDQ
410 420 430 440 450
EASCISAFEP PGPGSYERLS TGPGESKPDV LAGKQEASAD CQDVISVTAN
460 470 480 490 500
RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD
510 520 530 540 550
HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS
560 570 580 590 600
ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW
610 620 630 640 650
FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL
660 670 680 690 700
ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS
710 720 730 740 750
DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT
760 770 780 790 800
RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT
810 820 830 840 850
EYTSGYERED PVIQWFWEVV EDMTQEERVL LLQFVTGSSR VPHGGFANIM
860 870 880 890 900
GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH

CGSYGYTMA
Length:909
Mass (Da):102,159
Last modified:April 20, 2010 - v1
Checksum:i60C1FC2E282C2F99
GO

Sequence databases

RefSeqiNP_001102009.2. NM_001108539.3.
UniGeneiRn.28116.

Genome annotation databases

EnsembliENSRNOT00000000365; ENSRNOP00000000365; ENSRNOG00000000327.
GeneIDi361866.
KEGGirno:361866.
UCSCiRGD:1306114. rat.

Cross-referencesi

Sequence databases

RefSeqiNP_001102009.2. NM_001108539.3.
UniGeneiRn.28116.

3D structure databases

ProteinModelPortaliD3ZBM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000000365.

Proteomic databases

PaxDbiD3ZBM7.
PeptideAtlasiD3ZBM7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000000365; ENSRNOP00000000365; ENSRNOG00000000327.
GeneIDi361866.
KEGGirno:361866.
UCSCiRGD:1306114. rat.

Organism-specific databases

CTDi57531.
RGDi1306114. Hace1.

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
KOG4177. Eukaryota.
COG0666. LUCA.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
InParanoidiD3ZBM7.
KOiK12166.
OMAiTCEILIQ.
OrthoDBiEOG091G025R.
TreeFamiTF323417.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiD3ZBM7.

Gene expression databases

BgeeiENSRNOG00000000327.
GenevisibleiD3ZBM7. RN.

Family and domain databases

Gene3Di1.25.40.20. 3 hits.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF12796. Ank_2. 3 hits.
PF00632. HECT. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 5 hits.
PS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHACE1_RAT
AccessioniPrimary (citable) accession number: D3ZBM7
Secondary accession number(s): D3ZBM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2012
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.