D3ZBE5 (NEK7_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase Nek7
EC=2.7.11.1

Alternative name(s):
Never in mitosis A-related kinase 7
Short name=NimA-related protein kinase 7

Gene names

Name:

Nek7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. releasing the autoinhibitory function of Tyr-97 By similarity. Ref.2
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium.
Enzyme regulation	Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97 By similarity.
Subunit structure	Monomer. Interacts with NEK9 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm. Cytoplasm › cytoskeleton › spindle pole By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Note: Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody By similarity.
Tissue specificity	Highly expressed in the liver. Ref.2
Domain	Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 By similarity. The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity By similarity.
Post-translational modification	Phosphorylation at Ser-195 required for its activation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell spindle pole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Serine/threonine-protein kinase Nek7		PRO_0000412823
Regions
	Domain	34 – 299	266	Protein kinase
	Nucleotide binding	40 – 48	9	ATP By similarity
	Region	20 – 33	14	NTE motif By similarity
Sites
	Active site	161	1	Proton acceptor By similarity
	Binding site	63	1	ATP By similarity
	Site	97	1	Autoinhibitory By similarity
Amino acid modifications
	Modified residue	195	1	Phosphoserine; by NEK9 By similarity

Sequences

	Sequence		Length	Mass (Da)
	D3ZBE5 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: 1773BB6E19DFBEDA	FASTA	302	34,529
	10 20 30 40 50 60 MDEQPQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR ASCLLDGVPV 70 80 90 100 110 120 ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS FIEDNELNIV LELADAGDLS 130 140 150 160 170 180 RMIKHFKKQK RLIPERTVWK YFVQLCSALD HMHSRRVMHR DIKPANVFIT ATGVVKLGDL 190 200 210 220 230 240 GLGRFFSSKT TAAHSLVGTP YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK 250 260 270 280 290 300 MNLYSLCKKI EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA SS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase." Belham C., Comb M.J., Avruch J. Curr. Biol. 11:1155-1167(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473958 Genomic DNA. Translation: EDM09639.1.
IPI	IPI00363843.
RefSeq	NP_001101816.1. NM_001108346.2.
UniGene	Rn.211490.
3D structure databases
ProteinModelPortal	D3ZBE5.
ModBase	Search...
Proteomic databases
PRIDE	D3ZBE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000000817; ENSRNOP00000000817; ENSRNOG00000000657.
GeneID	360850.
KEGG	rno:360850.
UCSC	RGD:1311160. rat.
Organism-specific databases
CTD	140609.
RGD	1311160. Nek7.
Phylogenomic databases
GeneTree	ENSGT00700000104074.
KO	K08857.
OMA	QLVNICI.
OrthoDB	EOG4NVZKN.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	674364.

Entry information

Entry name

NEK7_RAT

Accession

Primary (citable) accession number: D3ZBE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	April 20, 2010
Last modified:	March 6, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program