Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D3ZBE5 (NEK7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase Nek7

EC=2.7.11.1
Alternative name(s):
Never in mitosis A-related kinase 7
Short name=NimA-related protein kinase 7
Gene names
Name:Nek7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1. releasing the autoinhibitory function of Tyr-97 By similarity. Ref.2

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium.

Enzyme regulation

Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97 By similarity.

Subunit structure

Monomer. Interacts with NEK9 By similarity.

Subcellular location

Nucleus By similarity. Cytoplasm. Cytoplasmcytoskeletonspindle pole By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Note: Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody By similarity.

Tissue specificity

Highly expressed in the liver. Ref.2

Domain

Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9 By similarity.

The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity By similarity.

Post-translational modification

Phosphorylation at Ser-195 required for its activation By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302Serine/threonine-protein kinase Nek7
PRO_0000412823

Regions

Domain34 – 299266Protein kinase
Nucleotide binding40 – 489ATP By similarity
Region20 – 3314NTE motif By similarity

Sites

Active site1611Proton acceptor By similarity
Binding site631ATP By similarity
Site971Autoinhibitory By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue1951Phosphoserine; by NEK9 By similarity

Sequences

Sequence LengthMass (Da)Tools
D3ZBE5 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 1773BB6E19DFBEDA

FASTA30234,529
        10         20         30         40         50         60 
MDEQPQGMQG PPVPQFQPQK ALRPDMGYNT LANFRIEKKI GRGQFSEVYR ASCLLDGVPV 

        70         80         90        100        110        120 
ALKKVQIFDL MDAKARADCI KEIDLLKQLN HPNVIKYYAS FIEDNELNIV LELADAGDLS 

       130        140        150        160        170        180 
RMIKHFKKQK RLIPERTVWK YFVQLCSALD HMHSRRVMHR DIKPANVFIT ATGVVKLGDL 

       190        200        210        220        230        240 
GLGRFFSSKT TAAHSLVGTP YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK 

       250        260        270        280        290        300 
MNLYSLCKKI EQCDYPPLPS DHYSEELRQL VNICINPDPE KRPDIAYVYD VAKRMHACTA 


SS 

« Hide

References

« Hide 'large scale' references
[1]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase."
Belham C., Comb M.J., Avruch J.
Curr. Biol. 11:1155-1167(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH473958 Genomic DNA. Translation: EDM09639.1.
RefSeqNP_001101816.1. NM_001108346.2.
XP_006249988.1. XM_006249926.1.
XP_006249989.1. XM_006249927.1.
UniGeneRn.211490.

3D structure databases

ProteinModelPortalD3ZBE5.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDED3ZBE5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000000817; ENSRNOP00000000817; ENSRNOG00000000657.
GeneID360850.
KEGGrno:360850.
UCSCRGD:1311160. rat.

Organism-specific databases

CTD140609.
RGD1311160. Nek7.

Phylogenomic databases

GeneTreeENSGT00720000108402.
KOK08857.
OMAQLVNICI.
OrthoDBEOG74J983.
PhylomeDBD3ZBE5.
TreeFamTF105135.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio674364.
PROD3ZBE5.

Entry information

Entry nameNEK7_RAT
AccessionPrimary (citable) accession number: D3ZBE5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: April 20, 2010
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families