Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine protease HTRA3

Gene

Htra3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei197 – 1971Charge relay systemBy similarity
Active sitei233 – 2331Charge relay systemBy similarity
Active sitei311 – 3111Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease HTRA3 (EC:3.4.21.-)
Alternative name(s):
High-temperature requirement factor A3
Pregnancy-related serine protease
Gene namesi
Name:Htra3
Synonyms:Prsp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1308120. Htra3.

Subcellular locationi

  • Secreted By similarity

  • Note: Secretion increased during decidualization of endometrial stromal cells.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 459436Serine protease HTRA3PRO_0000417598Add
BLAST

Proteomic databases

PaxDbiD3ZA76.

Expressioni

Tissue specificityi

Expressed in the ovary, essentially in granulosa cells in a follicle-stage specific manner. Highest levels found in large luteinizing granulosa cells.1 Publication

Developmental stagei

In the developing ovary, high expression, especially of the longer isoform, at 12 days of age after birth. Expression resticted to the interstitial cells surrounding the follicles. Levels are further increased during ovarian maturation.1 Publication

Gene expression databases

GenevisibleiD3ZA76. RN.

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with TGFB1; the interaction inhibits TGFB-mediated signaling. Interacts with BMP4; the interaction inhibits BMP4-mediated signaling. Interacts with TGFB2, GDF5 and MYH9 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010852.

Structurei

3D structure databases

ProteinModelPortaliD3ZA76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 9064IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini76 – 13459Kazal-likePROSITE-ProRule annotationAdd
BLAST
Domaini365 – 45086PDZPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 346166Serine proteaseBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 Kazal-like domain.PROSITE-ProRule annotation
Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00510000046315.
InParanoidiD3ZA76.
KOiK08785.
OMAiTSGIYVH.
OrthoDBiEOG7V1FR7.
TreeFamiTF323480.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR000867. IGFBP-like.
IPR002350. Kazal_dom.
IPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF00219. IGFBP. 1 hit.
PF07648. Kazal_2. 1 hit.
PF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00121. IB. 1 hit.
SM00280. KAZAL. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS51323. IGFBP_N_2. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3ZA76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQARALLPAT LATLATLAVS VLAREPPAAP CPARCDVSRC PSPRCPGGYV
60 70 80 90 100
PDLCNCCLVC AASEGEPCGR PLDSPCGDSL ECVRGVCRCR WTHTVCGTDG
110 120 130 140 150
HTYADVCALQ AASRRALQIS GTPVRQLQKG ACPSGLHQLT SPRYKFNFIA
160 170 180 190 200
DVVEKIAPAV VHIELFLRHP LFGRNVPLSS GSGFIMSEAG LIVTNAHVVS
210 220 230 240 250
SSNTASGRQQ LKVQLQNGDA YEATIQDIDK KSDIATILIH PNKKLPVLLL
260 270 280 290 300
GHSADLRPGE FVVAIGSPFA LQNTVTTGIV STAQRDGKEL GLRDSDMDYI
310 320 330 340 350
QTDAIINYGN SGGPLVNLDG EVIGINTLKV AAGISFAIPS DRITRFLSEF
360 370 380 390 400
QDKHVKDWKK RFIGIRMRTI TPSLVEELKT ANPDFPAVSS GIYVQEVVPN
410 420 430 440 450
SPSQRGGIQD GDIIVKVNGR PLVDSSELQE AVLNESSLLL EVRRGNDDLL

FSIMPEVVM
Length:459
Mass (Da):49,257
Last modified:April 20, 2010 - v1
Checksum:iBFD7DDD34305C818
GO
Isoform 2 (identifier: D3ZA76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-363: DWKKRFI → ALSRALH
     364-459: Missing.

Show »
Length:363
Mass (Da):38,601
Checksum:i6E54DB4B5DB9C5CB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei357 – 3637DWKKRFI → ALSRALH in isoform 2. 1 PublicationVSP_043827
Alternative sequencei364 – 45996Missing in isoform 2. 1 PublicationVSP_043828Add
BLAST

Sequence databases

RefSeqiNP_001257956.1. NM_001271027.1. [D3ZA76-1]
UniGeneiRn.15228.

Genome annotation databases

EnsembliENSRNOT00000010852; ENSRNOP00000010852; ENSRNOG00000008182. [D3ZA76-1]
GeneIDi360959.
KEGGirno:360959.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

RefSeqiNP_001257956.1. NM_001271027.1. [D3ZA76-1]
UniGeneiRn.15228.

3D structure databases

ProteinModelPortaliD3ZA76.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010852.

Proteomic databases

PaxDbiD3ZA76.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010852; ENSRNOP00000010852; ENSRNOG00000008182. [D3ZA76-1]
GeneIDi360959.
KEGGirno:360959.

Organism-specific databases

CTDi94031.
RGDi1308120. Htra3.

Phylogenomic databases

eggNOGiKOG1320. Eukaryota.
COG0265. LUCA.
GeneTreeiENSGT00510000046315.
InParanoidiD3ZA76.
KOiK08785.
OMAiTSGIYVH.
OrthoDBiEOG7V1FR7.
TreeFamiTF323480.

Miscellaneous databases

NextBioi674721.
PROiD3ZA76.

Gene expression databases

GenevisibleiD3ZA76. RN.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_.
IPR000867. IGFBP-like.
IPR002350. Kazal_dom.
IPR001478. PDZ.
IPR009003. Peptidase_S1_PA.
IPR001940. Peptidase_S1C.
[Graphical view]
PfamiPF00219. IGFBP. 1 hit.
PF07648. Kazal_2. 1 hit.
PF13180. PDZ_2. 1 hit.
[Graphical view]
PRINTSiPR00834. PROTEASES2C.
SMARTiSM00121. IB. 1 hit.
SM00280. KAZAL. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF57184. SSF57184. 1 hit.
PROSITEiPS51323. IGFBP_N_2. 1 hit.
PS51465. KAZAL_2. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Evolutionary conservation of mammalian HTRA3 and its developmental regulation in the rat ovary."
    Bowden M., Drummond A.E., Salamonsen L.A., Findlay J.K., Nie G.
    J. Exp. Zool. B Mol. Dev. Evol. 312:701-713(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
    Strain: Brown Norway.

Entry informationi

Entry nameiHTRA3_RAT
AccessioniPrimary (citable) accession number: D3ZA76
Secondary accession number(s): D3ZLW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 20, 2010
Last modified: January 20, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.