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Protein

Serine protease HTRA3

Gene

Htra3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197Charge relay systemBy similarity1
Active sitei233Charge relay systemBy similarity1
Active sitei311Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease HTRA3 (EC:3.4.21.-)
Alternative name(s):
High-temperature requirement factor A3
Pregnancy-related serine protease
Gene namesi
Name:Htra3
Synonyms:Prsp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1308120 Htra3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000041759824 – 459Serine protease HTRA3Add BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi87 ↔ 107PROSITE-ProRule annotation
Disulfide bondi96 ↔ 132PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiD3ZA76

Expressioni

Tissue specificityi

Expressed in the ovary, essentially in granulosa cells in a follicle-stage specific manner. Highest levels found in large luteinizing granulosa cells.1 Publication

Developmental stagei

In the developing ovary, high expression, especially of the longer isoform, at 12 days of age after birth. Expression restricted to the interstitial cells surrounding the follicles. Levels are further increased during ovarian maturation.1 Publication

Gene expression databases

BgeeiENSRNOG00000008182
GenevisibleiD3ZA76 RN

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with TGFB1; the interaction inhibits TGFB-mediated signaling. Interacts with BMP4; the interaction inhibits BMP4-mediated signaling. Interacts with TGFB2, GDF5 and MYH9 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000010852

Structurei

3D structure databases

ProteinModelPortaliD3ZA76
SMRiD3ZA76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 90IGFBP N-terminalPROSITE-ProRule annotationAdd BLAST64
Domaini76 – 134Kazal-likePROSITE-ProRule annotationAdd BLAST59
Domaini365 – 450PDZPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni181 – 346Serine proteaseBy similarityAdd BLAST166

Sequence similaritiesi

Belongs to the peptidase S1C family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1320 Eukaryota
COG0265 LUCA
GeneTreeiENSGT00510000046315
InParanoidiD3ZA76
KOiK08785
OMAiTDGHTYA
OrthoDBiEOG091G0LXR
TreeFamiTF323480

Family and domain databases

InterProiView protein in InterPro
IPR009030 Growth_fac_rcpt_cys_sf
IPR000867 IGFBP-like
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR001478 PDZ
IPR036034 PDZ_sf
IPR009003 Peptidase_S1_PA
IPR001940 Peptidase_S1C
PfamiView protein in Pfam
PF00219 IGFBP, 1 hit
PF07648 Kazal_2, 1 hit
PF13180 PDZ_2, 1 hit
PRINTSiPR00834 PROTEASES2C
SMARTiView protein in SMART
SM00121 IB, 1 hit
SM00280 KAZAL, 1 hit
SM00228 PDZ, 1 hit
SUPFAMiSSF100895 SSF100895, 1 hit
SSF50156 SSF50156, 1 hit
SSF50494 SSF50494, 1 hit
SSF57184 SSF57184, 1 hit
PROSITEiView protein in PROSITE
PS51323 IGFBP_N_2, 1 hit
PS51465 KAZAL_2, 1 hit
PS50106 PDZ, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3ZA76-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQARALLPAT LATLATLAVS VLAREPPAAP CPARCDVSRC PSPRCPGGYV
60 70 80 90 100
PDLCNCCLVC AASEGEPCGR PLDSPCGDSL ECVRGVCRCR WTHTVCGTDG
110 120 130 140 150
HTYADVCALQ AASRRALQIS GTPVRQLQKG ACPSGLHQLT SPRYKFNFIA
160 170 180 190 200
DVVEKIAPAV VHIELFLRHP LFGRNVPLSS GSGFIMSEAG LIVTNAHVVS
210 220 230 240 250
SSNTASGRQQ LKVQLQNGDA YEATIQDIDK KSDIATILIH PNKKLPVLLL
260 270 280 290 300
GHSADLRPGE FVVAIGSPFA LQNTVTTGIV STAQRDGKEL GLRDSDMDYI
310 320 330 340 350
QTDAIINYGN SGGPLVNLDG EVIGINTLKV AAGISFAIPS DRITRFLSEF
360 370 380 390 400
QDKHVKDWKK RFIGIRMRTI TPSLVEELKT ANPDFPAVSS GIYVQEVVPN
410 420 430 440 450
SPSQRGGIQD GDIIVKVNGR PLVDSSELQE AVLNESSLLL EVRRGNDDLL

FSIMPEVVM
Length:459
Mass (Da):49,257
Last modified:April 20, 2010 - v1
Checksum:iBFD7DDD34305C818
GO
Isoform 2 (identifier: D3ZA76-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     357-363: DWKKRFI → ALSRALH
     364-459: Missing.

Show »
Length:363
Mass (Da):38,601
Checksum:i6E54DB4B5DB9C5CB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043827357 – 363DWKKRFI → ALSRALH in isoform 2. 1 Publication7
Alternative sequenceiVSP_043828364 – 459Missing in isoform 2. 1 PublicationAdd BLAST96

Sequence databases

RefSeqiNP_001257956.1, NM_001271027.1 [D3ZA76-1]
XP_017454804.1, XM_017599315.1 [D3ZA76-2]
UniGeneiRn.15228

Genome annotation databases

EnsembliENSRNOT00000010852; ENSRNOP00000010852; ENSRNOG00000008182 [D3ZA76-1]
GeneIDi360959
KEGGirno:360959

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHTRA3_RAT
AccessioniPrimary (citable) accession number: D3ZA76
Secondary accession number(s): D3ZLW3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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