Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Kelch-like protein 20

Gene

Klhl20

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport, Ubl conjugation pathway

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 20
Gene namesi
Name:Klhl20
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1309490. Klhl20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 609609Kelch-like protein 20PRO_0000396628Add
BLAST

Proteomic databases

PaxDbiD3Z8N4.
PRIDEiD3Z8N4.

Expressioni

Gene expression databases

GenevisibleiD3Z8N4. RN.

Interactioni

Subunit structurei

Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL20 and RBX1. Interacts with PDZ-RhoGEF/ARHGEF11, DAPK1 and CORO7. Interacts with F-actin. Interacts with IFN-gamma (IFNG) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003851.

Structurei

3D structure databases

ProteinModelPortaliD3Z8N4.
SMRiD3Z8N4. Positions 50-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 13568BTBPROSITE-ProRule annotationAdd
BLAST
Domaini170 – 272103BACKAdd
BLAST
Repeati319 – 36547Kelch 1Add
BLAST
Repeati367 – 41347Kelch 2Add
BLAST
Repeati414 – 46047Kelch 3Add
BLAST
Repeati462 – 50746Kelch 4Add
BLAST
Repeati509 – 55446Kelch 5Add
BLAST
Repeati556 – 60146Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
InParanoidiD3Z8N4.
KOiK10457.
OMAiENRWHTV.
OrthoDBiEOG7ZWD17.
PhylomeDBiD3Z8N4.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3Z8N4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKPMRRCT NIRPGETGMD VTSRCTLGDP NKLPEGVPQP ARMPYISDKH
60 70 80 90 100
PRQTLEVINL LRKHRELCDV VLVVGAKKIY AHRVILSACS PYFRAMFTGE
110 120 130 140 150
LAESRQTEVV IRDIDERAME LLIDFAYTSQ ITVEEGNVQT LLPAACLLQL
160 170 180 190 200
AEIQEACCEF LKRQLDPSNC LGIRAFADTH SCRELLRIAD KFTQHNFQEV
210 220 230 240 250
MESEEFMLLP ANQLIDIISS DELNVRSEEQ VFNAVMAWVK YSIQERRPQL
260 270 280 290 300
PQVLQHVRLP LLSPKFLVGT VGSDPLIKSD EECRDLVDEA KNYLLLPQER
310 320 330 340 350
PLMQGPRTRP RKPIRCGEVL FAVGGWCSGD AISSVERYDP QTNEWRMVAS
360 370 380 390 400
MSKRRCGVGV SVLDDLLYAV GGHDGSSYLN SVERYDPKTN QWSSDVAPTS
410 420 430 440 450
TCRTSVGVAV LGGFLYAVGG QDGVSCLNIV ERYDPKENKW TRVASMSTRR
460 470 480 490 500
LGVAVAVLGG FLYAVGGSDG TSPLNTVERY NPQENRWHTI APMGTRRKHL
510 520 530 540 550
GCAVYQDMIY AVGGRDDTTE LSSAERYNPR TNQWSPVVAM TSRRSGVGLA
560 570 580 590 600
VVNGQLMAVG GFDGTTYLKT IEVFDPDANT WRLYGGMNYR RLGGGVGVIK

MTHCESHIW
Length:609
Mass (Da):67,955
Last modified:April 20, 2010 - v1
Checksum:iFA05E3FE8341B422
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473958 Genomic DNA. Translation: EDM09422.1.
RefSeqiNP_001100662.1. NM_001107192.1.
XP_006250174.1. XM_006250112.2.
UniGeneiRn.44191.

Genome annotation databases

EnsembliENSRNOT00000003851; ENSRNOP00000003851; ENSRNOG00000002875.
ENSRNOT00000076954; ENSRNOP00000068007; ENSRNOG00000002875.
GeneIDi304920.
KEGGirno:304920.
UCSCiRGD:1309490. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473958 Genomic DNA. Translation: EDM09422.1.
RefSeqiNP_001100662.1. NM_001107192.1.
XP_006250174.1. XM_006250112.2.
UniGeneiRn.44191.

3D structure databases

ProteinModelPortaliD3Z8N4.
SMRiD3Z8N4. Positions 50-599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000003851.

Proteomic databases

PaxDbiD3Z8N4.
PRIDEiD3Z8N4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000003851; ENSRNOP00000003851; ENSRNOG00000002875.
ENSRNOT00000076954; ENSRNOP00000068007; ENSRNOG00000002875.
GeneIDi304920.
KEGGirno:304920.
UCSCiRGD:1309490. rat.

Organism-specific databases

CTDi27252.
RGDi1309490. Klhl20.

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
InParanoidiD3Z8N4.
KOiK10457.
OMAiENRWHTV.
OrthoDBiEOG7ZWD17.
PhylomeDBiD3Z8N4.
TreeFamiTF329218.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi653837.
PROiD3Z8N4.

Gene expression databases

GenevisibleiD3Z8N4. RN.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "PDZ-RhoGEF ubiquitination by Cullin3-KLHL20 controls neurotrophin-induced neurite outgrowth."
    Lin M.Y., Lin Y.M., Kao T.C., Chuang H.H., Chen R.H.
    J. Cell Biol. 193:985-994(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKLH20_RAT
AccessioniPrimary (citable) accession number: D3Z8N4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: April 20, 2010
Last modified: May 11, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.