Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitotic spindle assembly checkpoint protein MAD2B

Gene

Mad2l2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-110312. Translesion synthesis by REV1.
R-RNO-5655862. Translesion synthesis by POLK.
R-RNO-5656121. Translesion synthesis by POLI.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle assembly checkpoint protein MAD2B
Alternative name(s):
Mitotic arrest deficient 2-like protein 2
Short name:
MAD2-like protein 2
Gene namesi
Name:Mad2l2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1307499. Mad2l2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Mitotic spindle assembly checkpoint protein MAD2BPRO_0000405244Add
BLAST

Proteomic databases

PaxDbiD3Z8D9.

Expressioni

Gene expression databases

ExpressionAtlasiD3Z8D9. baseline and differential.
GenevisibleiD3Z8D9. RN.

Interactioni

Subunit structurei

Homooligomer. Interacts with REV1. Interacts with ADAM9. Interacts with CHAMP1. Interacts with REV3L. Interacts with FZR1 (in complex with the anaphase promoting complex APC). May interact with CDC20. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with PRCC; the interaction is direct. Interacts with POGZ (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052521.

Structurei

3D structure databases

ProteinModelPortaliD3Z8D9.
SMRiD3Z8D9. Positions 1-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 203191HORMAPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 155135Mediates interaction with REV1 and REV3L and homodimerizationBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 HORMA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3186. Eukaryota.
ENOG4111I7Q. LUCA.
GeneTreeiENSGT00500000044946.
InParanoidiD3Z8D9.
KOiK13728.
OMAiNNNPPGC.
OrthoDBiEOG7SN8FC.
PhylomeDBiD3Z8D9.
TreeFamiTF101085.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
[Graphical view]
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3Z8D9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTLTRQDLN FGQVVADVLS EFLEVAVHLI LYVREVYPVG IFQKRKKYNV
60 70 80 90 100
PVQMSCHPEL NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF
110 120 130 140 150
EITQPPLLSI NSDSLLSHVE QLLRAFILKI SVCDAVLDHN PPGCTFTVLV
160 170 180 190 200
HTREAATRNM EKIQVIKDFP WILADEQDVH MHDPRLIPLK TMTSDILKMQ
210
LYVEERAHKN S
Length:211
Mass (Da):24,402
Last modified:April 20, 2010 - v1
Checksum:i0E47D0D8AC28AB5C
GO
Isoform 2 (identifier: D3Z8D9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-211: NSDSLLSHVE...YVEERAHKNS → KLGGLKQTVS...SKMSTCMIPG

Show »
Length:234
Mass (Da):26,447
Checksum:i115FDEA637875E63
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei111 – 211101NSDSL…AHKNS → KLGGLKQTVSLTVLGSWRYA DLSGTLGVLSTAEYLSDLKG PLYPTSIQSVQTPSCLMWSS CFEPSFLRLVCVTLSSTIIL QAARLQFSCTQEKLPLGTWR RYRSSRTSPGSWQMSKMSTC MIPG in isoform 2. 1 PublicationVSP_040650Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL81101.1.
BC087687 mRNA. Translation: AAH87687.1.
RefSeqiNP_001012106.1. NM_001012106.1. [D3Z8D9-2]
XP_006239452.1. XM_006239390.2. [D3Z8D9-2]
XP_006239453.1. XM_006239391.2. [D3Z8D9-1]
XP_008762478.1. XM_008764256.1. [D3Z8D9-2]
UniGeneiRn.27237.

Genome annotation databases

EnsembliENSRNOT00000055658; ENSRNOP00000052521; ENSRNOG00000009134. [D3Z8D9-2]
ENSRNOT00000083678; ENSRNOP00000070808; ENSRNOG00000009134. [D3Z8D9-1]
GeneIDi313702.
KEGGirno:313702.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA. Translation: EDL81101.1.
BC087687 mRNA. Translation: AAH87687.1.
RefSeqiNP_001012106.1. NM_001012106.1. [D3Z8D9-2]
XP_006239452.1. XM_006239390.2. [D3Z8D9-2]
XP_006239453.1. XM_006239391.2. [D3Z8D9-1]
XP_008762478.1. XM_008764256.1. [D3Z8D9-2]
UniGeneiRn.27237.

3D structure databases

ProteinModelPortaliD3Z8D9.
SMRiD3Z8D9. Positions 1-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052521.

Proteomic databases

PaxDbiD3Z8D9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000055658; ENSRNOP00000052521; ENSRNOG00000009134. [D3Z8D9-2]
ENSRNOT00000083678; ENSRNOP00000070808; ENSRNOG00000009134. [D3Z8D9-1]
GeneIDi313702.
KEGGirno:313702.

Organism-specific databases

CTDi10459.
RGDi1307499. Mad2l2.

Phylogenomic databases

eggNOGiKOG3186. Eukaryota.
ENOG4111I7Q. LUCA.
GeneTreeiENSGT00500000044946.
InParanoidiD3Z8D9.
KOiK13728.
OMAiNNNPPGC.
OrthoDBiEOG7SN8FC.
PhylomeDBiD3Z8D9.
TreeFamiTF101085.

Enzyme and pathway databases

ReactomeiR-RNO-110312. Translesion synthesis by REV1.
R-RNO-5655862. Translesion synthesis by POLK.
R-RNO-5656121. Translesion synthesis by POLI.

Miscellaneous databases

PROiD3Z8D9.

Gene expression databases

ExpressionAtlasiD3Z8D9. baseline and differential.
GenevisibleiD3Z8D9. RN.

Family and domain databases

Gene3Di3.30.900.10. 1 hit.
InterProiIPR003511. HORMA_dom.
[Graphical view]
PfamiPF02301. HORMA. 1 hit.
[Graphical view]
SUPFAMiSSF56019. SSF56019. 1 hit.
PROSITEiPS50815. HORMA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Ovary.

Entry informationi

Entry nameiMD2L2_RAT
AccessioniPrimary (citable) accession number: D3Z8D9
Secondary accession number(s): Q5PPH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: April 20, 2010
Last modified: July 6, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.