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Protein

Mitotic spindle assembly checkpoint protein MAD2B

Gene

Mad2l2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, DNA damage, Mitosis, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-110312 Translesion synthesis by REV1
R-RNO-5655862 Translesion synthesis by POLK
R-RNO-5656121 Translesion synthesis by POLI

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic spindle assembly checkpoint protein MAD2B
Alternative name(s):
Mitotic arrest deficient 2-like protein 2
Short name:
MAD2-like protein 2
Gene namesi
Name:Mad2l2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1307499 Mad2l2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004052441 – 211Mitotic spindle assembly checkpoint protein MAD2BAdd BLAST211

Proteomic databases

PaxDbiD3Z8D9

Expressioni

Gene expression databases

BgeeiENSRNOG00000009134
ExpressionAtlasiD3Z8D9 baseline and differential
GenevisibleiD3Z8D9 RN

Interactioni

Subunit structurei

Homooligomer. Interacts with REV1. Interacts with ADAM9. Interacts with CHAMP1. Interacts with REV3L. Interacts with FZR1 (in complex with the anaphase promoting complex APC). May interact with CDC20. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with PRCC; the interaction is direct. Interacts with POGZ (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052521

Structurei

3D structure databases

ProteinModelPortaliD3Z8D9
SMRiD3Z8D9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 203HORMAPROSITE-ProRule annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 155Mediates interaction with REV1 and REV3L and homodimerizationBy similarityAdd BLAST135

Phylogenomic databases

eggNOGiKOG3186 Eukaryota
ENOG4111I7Q LUCA
GeneTreeiENSGT00500000044946
InParanoidiD3Z8D9
KOiK13728
OMAiPWIVADE
OrthoDBiEOG091G0KY2
PhylomeDBiD3Z8D9
TreeFamiTF101085

Family and domain databases

Gene3Di3.30.900.10, 1 hit
InterProiView protein in InterPro
IPR003511 HORMA_dom
IPR036570 HORMA_dom_sf
PfamiView protein in Pfam
PF02301 HORMA, 1 hit
SUPFAMiSSF56019 SSF56019, 1 hit
PROSITEiView protein in PROSITE
PS50815 HORMA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3Z8D9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTTLTRQDLN FGQVVADVLS EFLEVAVHLI LYVREVYPVG IFQKRKKYNV
60 70 80 90 100
PVQMSCHPEL NQYIQDTLHC VKPLLEKNDV EKVVVVILDK EHRPVEKFVF
110 120 130 140 150
EITQPPLLSI NSDSLLSHVE QLLRAFILKI SVCDAVLDHN PPGCTFTVLV
160 170 180 190 200
HTREAATRNM EKIQVIKDFP WILADEQDVH MHDPRLIPLK TMTSDILKMQ
210
LYVEERAHKN S
Length:211
Mass (Da):24,402
Last modified:April 20, 2010 - v1
Checksum:i0E47D0D8AC28AB5C
GO
Isoform 2 (identifier: D3Z8D9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-211: NSDSLLSHVE...YVEERAHKNS → KLGGLKQTVS...SKMSTCMIPG

Show »
Length:234
Mass (Da):26,447
Checksum:i115FDEA637875E63
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040650111 – 211NSDSL…AHKNS → KLGGLKQTVSLTVLGSWRYA DLSGTLGVLSTAEYLSDLKG PLYPTSIQSVQTPSCLMWSS CFEPSFLRLVCVTLSSTIIL QAARLQFSCTQEKLPLGTWR RYRSSRTSPGSWQMSKMSTC MIPG in isoform 2. 1 PublicationAdd BLAST101

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473968 Genomic DNA Translation: EDL81101.1
BC087687 mRNA Translation: AAH87687.1
RefSeqiNP_001012106.1, NM_001012106.1 [D3Z8D9-2]
XP_006239452.1, XM_006239390.3 [D3Z8D9-2]
XP_006239453.1, XM_006239391.3 [D3Z8D9-1]
XP_008762478.1, XM_008764256.2 [D3Z8D9-2]
UniGeneiRn.27237

Genome annotation databases

EnsembliENSRNOT00000055658; ENSRNOP00000052521; ENSRNOG00000009134 [D3Z8D9-2]
ENSRNOT00000083678; ENSRNOP00000070808; ENSRNOG00000009134 [D3Z8D9-1]
GeneIDi313702
KEGGirno:313702

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMD2L2_RAT
AccessioniPrimary (citable) accession number: D3Z8D9
Secondary accession number(s): Q5PPH8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: April 20, 2010
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health