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D3Z7P3

- GLSK_MOUSE

UniProt

D3Z7P3 - GLSK_MOUSE

Protein

Glutaminase kidney isoform, mitochondrial

Gene

Gls

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.3 Publications

    Catalytic activityi

    L-glutamine + H2O = L-glutamate + NH3.1 Publication

    Enzyme regulationi

    Isoform 1 and isoform 2 are activated by phosphate, due to increased affinity for glutamine. At phosphate concentrations above 10 mM, isoform 2 is more efficient than isoform 1.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei291 – 2911Substrate
    Binding sitei340 – 3401Substrate
    Binding sitei386 – 3861Substrate
    Binding sitei393 – 3931Substrate
    Binding sitei419 – 4191Substrate
    Binding sitei471 – 4711Substrate
    Binding sitei489 – 4891Substrate; via amide nitrogen

    GO - Molecular functioni

    1. glutaminase activity Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB
    2. glutamine catabolic process Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB
    4. regulation of respiratory gaseous exchange by neurological system process Source: MGI
    5. suckling behavior Source: MGI
    6. synaptic transmission Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutaminase kidney isoform, mitochondrial (EC:3.5.1.2)
    Short name:
    GLS
    Gene namesi
    Name:Gls
    Synonyms:Gls1, Kiaa0838
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:95752. Gls.

    Subcellular locationi

    Isoform 1 : Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Death during the first days after birth. Pups appear normal and display normal levels of activity, but their activity is disorganized. Pups do not orient to the dam, do not succeed in grasping a nipple and do not feed properly. In addition, they display altered respiration.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi394 – 3941F → S: Impairs tetramerization and promotes formation of homodimers. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1616MitochondrionBy similarityAdd
    BLAST
    Chaini17 – 674658Glutaminase kidney isoform, mitochondrialPRO_0000417583Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei135 – 1351N6-succinyllysine1 Publication
    Modified residuei169 – 1691N6-succinyllysine1 Publication
    Modified residuei316 – 3161N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiD3Z7P3.
    PRIDEiD3Z7P3.

    Expressioni

    Gene expression databases

    BgeeiD3Z7P3.

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.2 Publications

    Protein-protein interaction databases

    DIPiDIP-60006N.
    STRINGi10090.ENSMUSP00000110158.

    Structurei

    Secondary structure

    1
    674
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi145 – 1517
    Beta strandi157 – 1604
    Helixi161 – 17010
    Helixi178 – 1803
    Helixi181 – 19313
    Helixi202 – 2098
    Helixi210 – 2123
    Helixi213 – 2208
    Beta strandi224 – 2274
    Helixi229 – 24315
    Beta strandi249 – 2513
    Helixi256 – 2594
    Beta strandi267 – 2726
    Beta strandi277 – 2826
    Helixi290 – 2934
    Helixi294 – 31320
    Beta strandi321 – 3288
    Beta strandi332 – 3354
    Helixi340 – 34910
    Turni350 – 3534
    Helixi356 – 37015
    Turni371 – 3733
    Beta strandi376 – 3783
    Helixi380 – 3889
    Helixi391 – 40212
    Helixi412 – 42312
    Beta strandi425 – 4273
    Helixi429 – 44012
    Turni441 – 4433
    Turni446 – 4483
    Helixi455 – 46814
    Helixi471 – 4733
    Helixi474 – 4807
    Beta strandi485 – 4873
    Beta strandi491 – 4977
    Turni498 – 5003
    Beta strandi501 – 5066
    Beta strandi508 – 5103
    Beta strandi514 – 5163
    Helixi517 – 52913

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3SS3X-ray2.42A/B/C/D128-555[»]
    3SS4X-ray2.85A/B/C/D128-555[»]
    3SS5X-ray2.80A/B/C/D128-555[»]
    4JKTX-ray2.77A/B/C/D128-555[»]
    ProteinModelPortaliD3Z7P3.
    SMRiD3Z7P3. Positions 142-646.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati590 – 61930ANK 1Add
    BLAST
    Repeati624 – 65330ANK 2Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi59 – 624Poly-Gly
    Compositional biasi91 – 999Poly-Gln

    Sequence similaritiesi

    Belongs to the glutaminase family.Curated
    Contains 2 ANK repeats.Curated

    Keywords - Domaini

    ANK repeat, Repeat, Transit peptide

    Phylogenomic databases

    GeneTreeiENSGT00390000010463.
    HOGENOMiHOG000216891.
    KOiK01425.
    OMAiGMDMEQR.
    OrthoDBiEOG7S4X5F.
    TreeFamiTF313359.

    Family and domain databases

    Gene3Di1.25.40.20. 2 hits.
    3.40.710.10. 1 hit.
    HAMAPiMF_00313. Glutaminase.
    InterProiIPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view]
    PANTHERiPTHR12544. PTHR12544. 1 hit.
    PfamiPF12796. Ank_2. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view]
    SMARTiSM00248. ANK. 2 hits.
    [Graphical view]
    SUPFAMiSSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
    PROSITEiPS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: D3Z7P3-1) [UniParc]FASTAAdd to Basket

    Also known as: KGA

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMRLRGSAML RELLLRPPAA VGAVLRRAQP LGTLCRRPRG GSRPTAGLVA    50
    AARLHPWWGG GGRAKGPGAG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP 100
    GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKIKQGL LPSLEDLLFY 150
    TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML 200
    DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK 250
    VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI 300
    AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI 350
    KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL 400
    KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE 450
    RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV 500
    MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE 550
    GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA 600
    EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ 650
    YTPQGDSDDG KGNQTVHKNL DGLL 674
    Length:674
    Mass (Da):73,964
    Last modified:April 20, 2010 - v1
    Checksum:iC46824C83E1D9639
    GO
    Isoform 2 (identifier: D3Z7P3-2) [UniParc]FASTAAdd to Basket

    Also known as: Glutaminase C, GAC

    The sequence of this isoform differs from the canonical sequence as follows:
         556-674: VKSVINLLFA...TVHKNLDGLL → HSFGPLDYES...YRMESLGERS

    Show »
    Length:603
    Mass (Da):65,995
    Checksum:iE896A4EDA7287755
    GO

    Sequence cautioni

    The sequence BAD32317.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei556 – 674119VKSVI…LDGLL → HSFGPLDYESLQQELALKDT VWKKVSPESSDDTSTTVVYR MESLGERS in isoform 2. 1 PublicationVSP_043804Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK173039 mRNA. Translation: BAD32317.1. Different initiation.
    AC123752 Genomic DNA. No translation available.
    CCDSiCCDS35561.1. [D3Z7P3-1]
    CCDS48256.1. [D3Z7P3-2]
    RefSeqiNP_001074550.1. NM_001081081.2. [D3Z7P3-1]
    NP_001106854.1. NM_001113383.1. [D3Z7P3-2]
    UniGeneiMm.398608.

    Genome annotation databases

    EnsembliENSMUST00000114510; ENSMUSP00000110155; ENSMUSG00000026103. [D3Z7P3-2]
    ENSMUST00000114513; ENSMUSP00000110158; ENSMUSG00000026103. [D3Z7P3-1]
    GeneIDi14660.
    KEGGimmu:14660.
    UCSCiuc007aye.2. mouse. [D3Z7P3-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK173039 mRNA. Translation: BAD32317.1 . Different initiation.
    AC123752 Genomic DNA. No translation available.
    CCDSi CCDS35561.1. [D3Z7P3-1 ]
    CCDS48256.1. [D3Z7P3-2 ]
    RefSeqi NP_001074550.1. NM_001081081.2. [D3Z7P3-1 ]
    NP_001106854.1. NM_001113383.1. [D3Z7P3-2 ]
    UniGenei Mm.398608.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3SS3 X-ray 2.42 A/B/C/D 128-555 [» ]
    3SS4 X-ray 2.85 A/B/C/D 128-555 [» ]
    3SS5 X-ray 2.80 A/B/C/D 128-555 [» ]
    4JKT X-ray 2.77 A/B/C/D 128-555 [» ]
    ProteinModelPortali D3Z7P3.
    SMRi D3Z7P3. Positions 142-646.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60006N.
    STRINGi 10090.ENSMUSP00000110158.

    Proteomic databases

    MaxQBi D3Z7P3.
    PRIDEi D3Z7P3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000114510 ; ENSMUSP00000110155 ; ENSMUSG00000026103 . [D3Z7P3-2 ]
    ENSMUST00000114513 ; ENSMUSP00000110158 ; ENSMUSG00000026103 . [D3Z7P3-1 ]
    GeneIDi 14660.
    KEGGi mmu:14660.
    UCSCi uc007aye.2. mouse. [D3Z7P3-1 ]

    Organism-specific databases

    CTDi 2744.
    MGIi MGI:95752. Gls.
    Rougei Search...

    Phylogenomic databases

    GeneTreei ENSGT00390000010463.
    HOGENOMi HOG000216891.
    KOi K01425.
    OMAi GMDMEQR.
    OrthoDBi EOG7S4X5F.
    TreeFami TF313359.

    Miscellaneous databases

    ChiTaRSi GLS. mouse.
    NextBioi 286530.
    PROi D3Z7P3.
    SOURCEi Search...

    Gene expression databases

    Bgeei D3Z7P3.

    Family and domain databases

    Gene3Di 1.25.40.20. 2 hits.
    3.40.710.10. 1 hit.
    HAMAPi MF_00313. Glutaminase.
    InterProi IPR002110. Ankyrin_rpt.
    IPR020683. Ankyrin_rpt-contain_dom.
    IPR012338. Beta-lactam/transpept-like.
    IPR015868. Glutaminase.
    [Graphical view ]
    PANTHERi PTHR12544. PTHR12544. 1 hit.
    Pfami PF12796. Ank_2. 1 hit.
    PF04960. Glutaminase. 1 hit.
    [Graphical view ]
    SMARTi SM00248. ANK. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48403. SSF48403. 1 hit.
    SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
    PROSITEi PS50297. ANK_REP_REGION. 1 hit.
    PS50088. ANK_REPEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
      DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Tissue: Embryonic tail.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: C57BL/6J.
    3. "Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels."
      Buschdorf J.P., Li Chew L., Zhang B., Cao Q., Liang F.Y., Liou Y.C., Zhou Y.T., Low B.C.
      J. Cell Sci. 119:3337-3350(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATCAY.
    4. "Mice lacking brain/kidney phosphate-activated glutaminase have impaired glutamatergic synaptic transmission, altered breathing, disorganized goal-directed behavior and die shortly after birth."
      Masson J., Darmon M., Conjard A., Chuhma N., Ropert N., Thoby-Brisson M., Foutz A.S., Parrot S., Miller G.M., Jorisch R., Polan J., Hamon M., Hen R., Rayport S.
      J. Neurosci. 26:4660-4671(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION, SUBCELLULAR LOCATION.
    5. "Brain slices from glutaminase-deficient mice metabolize less glutamine: a cellular metabolomic study with carbon 13 NMR."
      El Hage M., Masson J., Conjard-Duplany A., Ferrier B., Baverel G., Martin G.
      J. Cereb. Blood Flow Metab. 32:816-824(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-135 AND LYS-169, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    7. "Mitochondrial localization and structure-based phosphate activation mechanism of glutaminase C with implications for cancer metabolism."
      Cassago A., Ferreira A.P., Ferreira I.M., Fornezari C., Gomes E.R., Greene K.S., Pereira H.M., Garratt R.C., Dias S.M., Ambrosio A.L.
      Proc. Natl. Acad. Sci. U.S.A. 109:1092-1097(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) OF 128-555 (ISOFORM 2) IN COMPLEX WITH GLUTAMATE AND PHOSPHATE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, ALTERNATIVE SPLICING, MUTAGENESIS OF PHE-394, SUBUNIT.

    Entry informationi

    Entry nameiGLSK_MOUSE
    AccessioniPrimary (citable) accession number: D3Z7P3
    Secondary accession number(s): E9PUF0, Q69ZX9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2012
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3