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Protein

Glutaminase kidney isoform, mitochondrial

Gene

Gls

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain.3 Publications

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.1 Publication

Enzyme regulationi

Isoform 1 and isoform 2 are activated by phosphate, due to increased affinity for glutamine. At phosphate concentrations above 10 mM, isoform 2 is more efficient than isoform 1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei291Substrate1
Binding sitei340Substrate1
Binding sitei386Substrate1
Binding sitei393Substrate1
Binding sitei419Substrate1
Binding sitei471Substrate1
Binding sitei489Substrate; via amide nitrogen1

GO - Molecular functioni

  • glutaminase activity Source: UniProtKB

GO - Biological processi

  • chemical synaptic transmission Source: MGI
  • glutamate biosynthetic process Source: UniProtKB
  • glutamine catabolic process Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
  • regulation of respiratory gaseous exchange by neurological system process Source: MGI
  • suckling behavior Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-70614. Amino acid synthesis and interconversion (transamination).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase kidney isoform, mitochondrial (EC:3.5.1.2)
Short name:
GLS
Gene namesi
Name:Gls
Synonyms:Gls1, Kiaa0838
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:95752. Gls.

Subcellular locationi

Isoform 1 :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Death during the first days after birth. Pups appear normal and display normal levels of activity, but their activity is disorganized. Pups do not orient to the dam, do not succeed in grasping a nipple and do not feed properly. In addition, they display altered respiration.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi394F → S: Impairs tetramerization and promotes formation of homodimers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 16MitochondrionBy similarityAdd BLAST16
ChainiPRO_000041758317 – 674Glutaminase kidney isoform, mitochondrialAdd BLAST658

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135N6-succinyllysineCombined sources1
Modified residuei169N6-succinyllysineCombined sources1
Modified residuei316N6-acetyllysineBy similarity1
Modified residuei657PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiD3Z7P3.
MaxQBiD3Z7P3.
PaxDbiD3Z7P3.
PeptideAtlasiD3Z7P3.
PRIDEiD3Z7P3.

PTM databases

iPTMnetiD3Z7P3.
PhosphoSitePlusiD3Z7P3.
SwissPalmiD3Z7P3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026103.
ExpressionAtlasiD3Z7P3. baseline and differential.
GenevisibleiD3Z7P3. MM.

Interactioni

Subunit structurei

Homotetramer. Interacts with ATCAY; the interaction is direct and may control GLS localization, negatively regulating its activity.2 Publications

Protein-protein interaction databases

BioGridi199955. 1 interactor.
DIPiDIP-60006N.
IntActiD3Z7P3. 1 interactor.
STRINGi10090.ENSMUSP00000110158.

Structurei

Secondary structure

1674
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi145 – 151Combined sources7
Beta strandi157 – 160Combined sources4
Helixi161 – 170Combined sources10
Helixi178 – 180Combined sources3
Helixi181 – 193Combined sources13
Helixi202 – 209Combined sources8
Helixi210 – 212Combined sources3
Helixi213 – 220Combined sources8
Beta strandi224 – 227Combined sources4
Helixi229 – 243Combined sources15
Beta strandi249 – 251Combined sources3
Helixi256 – 259Combined sources4
Beta strandi267 – 272Combined sources6
Beta strandi277 – 282Combined sources6
Helixi290 – 293Combined sources4
Helixi294 – 313Combined sources20
Beta strandi321 – 328Combined sources8
Beta strandi332 – 335Combined sources4
Helixi340 – 349Combined sources10
Turni350 – 353Combined sources4
Helixi356 – 370Combined sources15
Turni371 – 373Combined sources3
Beta strandi376 – 378Combined sources3
Helixi380 – 388Combined sources9
Helixi391 – 402Combined sources12
Helixi412 – 423Combined sources12
Beta strandi425 – 427Combined sources3
Helixi429 – 440Combined sources12
Turni441 – 443Combined sources3
Turni446 – 448Combined sources3
Helixi455 – 468Combined sources14
Helixi471 – 473Combined sources3
Helixi474 – 480Combined sources7
Beta strandi485 – 487Combined sources3
Beta strandi491 – 497Combined sources7
Turni498 – 500Combined sources3
Beta strandi501 – 506Combined sources6
Beta strandi508 – 510Combined sources3
Beta strandi514 – 516Combined sources3
Helixi517 – 529Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SS3X-ray2.42A/B/C/D128-602[»]
3SS4X-ray2.85A/B/C/D128-602[»]
3SS5X-ray2.80A/B/C/D128-602[»]
4JKTX-ray2.77A/B/C/D128-555[»]
ProteinModelPortaliD3Z7P3.
SMRiD3Z7P3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati590 – 619ANK 1Add BLAST30
Repeati624 – 653ANK 2Add BLAST30

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi59 – 62Poly-Gly4
Compositional biasi91 – 99Poly-Gln9

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 2 ANK repeats.Curated

Keywords - Domaini

ANK repeat, Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0506. Eukaryota.
COG2066. LUCA.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
InParanoidiD3Z7P3.
KOiK01425.
OMAiFMNKMAG.
OrthoDBiEOG091G03YU.
TreeFamiTF313359.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: D3Z7P3-1) [UniParc]FASTAAdd to basket
Also known as: KGA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMRLRGSAML RELLLRPPAA VGAVLRRAQP LGTLCRRPRG GSRPTAGLVA
60 70 80 90 100
AARLHPWWGG GGRAKGPGAG GLSSSPSEIL QELGKGGTPP QQQQQQQQQP
110 120 130 140 150
GASPPAAPGP KDSPGETDAF GNSEGKEMVA AGDNKIKQGL LPSLEDLLFY
160 170 180 190 200
TIAEGQEKIP VHKFITALKS TGLRTSDPRL KECMDMLRLT LQTTSDGVML
210 220 230 240 250
DKDLFKKCVQ SNIVLLTQAF RRKFVIPDFM SFTSHIDELY ESAKKQSGGK
260 270 280 290 300
VADYIPQLAK FSPDLWGVSV CTVDGQRHSI GDTKVPFCLQ SCVKPLKYAI
310 320 330 340 350
AVNDLGTEYV HRYVGKEPSG LRFNKLFLNE DDKPHNPMVN AGAIVVTSLI
360 370 380 390 400
KQGVNNAEKF DYVMQFLNKM AGNEYVGFSN ATFQSERESG DRNFAIGYYL
410 420 430 440 450
KEKKCFPEGT DMVGILDFYF QLCSIEVTCE SASVMAATLA NGGFCPITGE
460 470 480 490 500
RVLSPEAVRN TLSLMHSCGM YDFSGQFAFH VGLPAKSGVA GGILLVVPNV
510 520 530 540 550
MGMMCWSPPL DKMGNSVKGI HFCHDLVSLC NFHNYDNLRH FAKKLDPRRE
560 570 580 590 600
GGDQRVKSVI NLLFAAYTGD VSALRRFALS AMDMEQRDYD SRTALHVAAA
610 620 630 640 650
EGHVEVVKFL LEACKVNPFP KDRWNNTPMD EALHFGHHDV FKILQEYQVQ
660 670
YTPQGDSDDG KGNQTVHKNL DGLL
Length:674
Mass (Da):73,964
Last modified:April 20, 2010 - v1
Checksum:iC46824C83E1D9639
GO
Isoform 2 (identifier: D3Z7P3-2) [UniParc]FASTAAdd to basket
Also known as: Glutaminase C, GAC

The sequence of this isoform differs from the canonical sequence as follows:
     556-674: VKSVINLLFA...TVHKNLDGLL → HSFGPLDYES...YRMESLGERS

Show »
Length:603
Mass (Da):65,995
Checksum:iE896A4EDA7287755
GO

Sequence cautioni

The sequence BAD32317 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_043804556 – 674VKSVI…LDGLL → HSFGPLDYESLQQELALKDT VWKKVSPESSDDTSTTVVYR MESLGERS in isoform 2. 1 PublicationAdd BLAST119

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173039 mRNA. Translation: BAD32317.1. Different initiation.
AC123752 Genomic DNA. No translation available.
CCDSiCCDS35561.1. [D3Z7P3-1]
CCDS48256.1. [D3Z7P3-2]
RefSeqiNP_001074550.1. NM_001081081.2. [D3Z7P3-1]
NP_001106854.1. NM_001113383.1. [D3Z7P3-2]
UniGeneiMm.398608.

Genome annotation databases

EnsembliENSMUST00000114510; ENSMUSP00000110155; ENSMUSG00000026103. [D3Z7P3-2]
ENSMUST00000114513; ENSMUSP00000110158; ENSMUSG00000026103. [D3Z7P3-1]
GeneIDi14660.
KEGGimmu:14660.
UCSCiuc007aye.2. mouse. [D3Z7P3-1]
uc011wko.1. mouse. [D3Z7P3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173039 mRNA. Translation: BAD32317.1. Different initiation.
AC123752 Genomic DNA. No translation available.
CCDSiCCDS35561.1. [D3Z7P3-1]
CCDS48256.1. [D3Z7P3-2]
RefSeqiNP_001074550.1. NM_001081081.2. [D3Z7P3-1]
NP_001106854.1. NM_001113383.1. [D3Z7P3-2]
UniGeneiMm.398608.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SS3X-ray2.42A/B/C/D128-602[»]
3SS4X-ray2.85A/B/C/D128-602[»]
3SS5X-ray2.80A/B/C/D128-602[»]
4JKTX-ray2.77A/B/C/D128-555[»]
ProteinModelPortaliD3Z7P3.
SMRiD3Z7P3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199955. 1 interactor.
DIPiDIP-60006N.
IntActiD3Z7P3. 1 interactor.
STRINGi10090.ENSMUSP00000110158.

PTM databases

iPTMnetiD3Z7P3.
PhosphoSitePlusiD3Z7P3.
SwissPalmiD3Z7P3.

Proteomic databases

EPDiD3Z7P3.
MaxQBiD3Z7P3.
PaxDbiD3Z7P3.
PeptideAtlasiD3Z7P3.
PRIDEiD3Z7P3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114510; ENSMUSP00000110155; ENSMUSG00000026103. [D3Z7P3-2]
ENSMUST00000114513; ENSMUSP00000110158; ENSMUSG00000026103. [D3Z7P3-1]
GeneIDi14660.
KEGGimmu:14660.
UCSCiuc007aye.2. mouse. [D3Z7P3-1]
uc011wko.1. mouse. [D3Z7P3-2]

Organism-specific databases

CTDi2744.
MGIiMGI:95752. Gls.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0506. Eukaryota.
COG2066. LUCA.
GeneTreeiENSGT00390000010463.
HOGENOMiHOG000216891.
InParanoidiD3Z7P3.
KOiK01425.
OMAiFMNKMAG.
OrthoDBiEOG091G03YU.
TreeFamiTF313359.

Enzyme and pathway databases

ReactomeiR-MMU-210500. Glutamate Neurotransmitter Release Cycle.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-70614. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

ChiTaRSiGls. mouse.
PROiD3Z7P3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026103.
ExpressionAtlasiD3Z7P3. baseline and differential.
GenevisibleiD3Z7P3. MM.

Family and domain databases

Gene3Di1.25.40.20. 2 hits.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR012338. Beta-lactam/transpept-like.
IPR015868. Glutaminase.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF12796. Ank_2. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLSK_MOUSE
AccessioniPrimary (citable) accession number: D3Z7P3
Secondary accession number(s): E9PUF0, Q69ZX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.