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Protein

Protein disulfide-isomerase A2

Gene

Pdia2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins (By similarity).By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741NucleophileBy similarity
Sitei75 – 751Contributes to redox potential valueBy similarity
Sitei76 – 761Contributes to redox potential valueBy similarity
Active sitei77 – 771NucleophileBy similarity
Active sitei421 – 4211NucleophileBy similarity
Sitei422 – 4221Contributes to redox potential valueBy similarity
Sitei423 – 4231Contributes to redox potential valueBy similarity
Active sitei424 – 4241NucleophileBy similarity
Sitei485 – 4851Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: GO_Central
  2. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. peptidyl-proline hydroxylation Source: GOC
  3. protein folding Source: GO_Central
  4. regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: GO_Central
  5. response to endoplasmic reticulum stress Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Keywords - Ligandi

Lipid-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A2By similarity (EC:5.3.4.1)
Alternative name(s):
PDIpBy similarity
Gene namesi
Name:Pdia2Imported
Synonyms:PdipBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1916441. Pdia2.

Subcellular locationi

Endoplasmic reticulum lumen PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

  1. endoplasmic reticulum Source: GO_Central
  2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 527507Protein disulfide-isomerase A2Sequence AnalysisPRO_0000394671Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 77Redox-activePROSITE-ProRule annotationBy similarity
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi421 ↔ 424Redox-activePROSITE-ProRule annotationBy similarity
Glycosylationi518 – 5181N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiD3Z6P0.
PRIDEiD3Z6P0.

Expressioni

Tissue specificityi

Highly expressed in pancreas.1 Publication

Gene expression databases

BgeeiD3Z6P0.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.By similarity

Protein-protein interaction databases

BioGridi213283. 2 interactions.
STRINGi10090.ENSMUSP00000035584.

Structurei

3D structure databases

ProteinModelPortaliD3Z6P0.
SMRiD3Z6P0. Positions 43-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 155127Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini355 – 499145Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi524 – 5274Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Sequence Analysis
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00760000119201.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiD3Z6P0.
KOiK09581.
OMAiQEVPPDW.
OrthoDBiEOG7VHSX1.
PhylomeDBiD3Z6P0.
TreeFamiTF106381.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: D3Z6P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKQLLPVLL LLLGVSGSWG QGEEPGGPSE VLPEEPTGEE VPKEDGILVL
60 70 80 90 100
NHRTLSLALQ EHSALMVEFY APWCGHCKEL APEYSKAAAL LAAESAVVTL
110 120 130 140 150
AKVDGPAEPE LTKEFEVVGY PTLKFFQNGN RTNPEEYAGP KTAEGIAEWL
160 170 180 190 200
RRRVGPSATH LEDEEGVQAL MAKWDMVVIG FFQDLQGKDM ATFLALAKDA
210 220 230 240 250
LDMTFGFTDQ PQLFEKFGLT KDTVVLFKKF DEGRADFPVD KETGLDLGDL
260 270 280 290 300
SRFLVIHSMH LVTEFNSQTS PKIFAAKILN HLLLFVNQTL AQHRELLTDF
310 320 330 340 350
REAAPPFRGQ VLFVMVDVAA DNSHVLNYFG LKAEEAPTLR LINVETTKKY
360 370 380 390 400
APTGVIAITA ASVAAFCQAV LHGEIKHYLL SQEIPPDWDQ GPVKTLVSKN
410 420 430 440 450
FEQVAFDETK NVFVKFYAPW CSHCKEMAPA WEALAEKYKD REDIVIAELD
460 470 480 490 500
ATANELEAFS VLGYPTLKFF PAGPDRKVID YKSTRDLETF SKFLDSGGHL
510 520
PKEEPKEPAA SAPEAQANST LGPKEEL
Length:527
Mass (Da):58,316
Last modified:April 19, 2010 - v1
Checksum:iCF703D296B634441
GO
Isoform 21 Publication (identifier: D3Z6P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-186: Missing.

Show »
Length:524
Mass (Da):57,960
Checksum:i4233CC4C3942C17D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei184 – 1863Missing in isoform 2. 1 PublicationVSP_039293

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1.
CCDSiCCDS37510.1. [D3Z6P0-1]
RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
UniGeneiMm.32631.

Genome annotation databases

EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
GeneIDi69191.
KEGGimmu:69191.
UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
uc012ans.1. mouse. [D3Z6P0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1.
CCDSiCCDS37510.1. [D3Z6P0-1]
RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
UniGeneiMm.32631.

3D structure databases

ProteinModelPortaliD3Z6P0.
SMRiD3Z6P0. Positions 43-499.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213283. 2 interactions.
STRINGi10090.ENSMUSP00000035584.

Proteomic databases

MaxQBiD3Z6P0.
PRIDEiD3Z6P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
GeneIDi69191.
KEGGimmu:69191.
UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
uc012ans.1. mouse. [D3Z6P0-2]

Organism-specific databases

CTDi64714.
MGIiMGI:1916441. Pdia2.

Phylogenomic databases

GeneTreeiENSGT00760000119201.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiD3Z6P0.
KOiK09581.
OMAiQEVPPDW.
OrthoDBiEOG7VHSX1.
PhylomeDBiD3Z6P0.
TreeFamiTF106381.

Miscellaneous databases

ChiTaRSiPdia2. mouse.
NextBioi328852.
PROiD3Z6P0.
SOURCEiSearch...

Gene expression databases

BgeeiD3Z6P0.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Molecular characterization of a pancreas-specific protein disulfide isomerase, PDIp."
    Desilva M.G., Notkins A.L., Lan M.S.
    DNA Cell Biol. 16:269-274(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GLYCOSYLATION.

Entry informationi

Entry nameiPDIA2_MOUSE
AccessioniPrimary (citable) accession number: D3Z6P0
Secondary accession number(s): Q14AV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 14, 2010
Last sequence update: April 19, 2010
Last modified: February 3, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.