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D3Z6P0

- PDIA2_MOUSE

UniProt

D3Z6P0 - PDIA2_MOUSE

Protein

Protein disulfide-isomerase A2

Gene

Pdia2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins By similarity.By similarity

    Catalytic activityi

    Catalyzes the rearrangement of -S-S- bonds in proteins.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei74 – 741NucleophileBy similarity
    Sitei75 – 751Contributes to redox potential valueBy similarity
    Sitei76 – 761Contributes to redox potential valueBy similarity
    Active sitei77 – 771NucleophileBy similarity
    Active sitei421 – 4211NucleophileBy similarity
    Sitei422 – 4221Contributes to redox potential valueBy similarity
    Sitei423 – 4231Contributes to redox potential valueBy similarity
    Active sitei424 – 4241NucleophileBy similarity
    Sitei485 – 4851Lowers pKa of C-terminal Cys of second active siteBy similarity

    GO - Molecular functioni

    1. protein disulfide isomerase activity Source: RefGenome
    2. steroid binding Source: UniProtKB-KW

    GO - Biological processi

    1. cell redox homeostasis Source: InterPro
    2. peptidyl-proline hydroxylation Source: GOC
    3. protein folding Source: RefGenome
    4. regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: RefGenome
    5. response to endoplasmic reticulum stress Source: RefGenome

    Keywords - Molecular functioni

    Chaperone, Isomerase

    Keywords - Ligandi

    Lipid-binding, Steroid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein disulfide-isomerase A2By similarity (EC:5.3.4.1)
    Alternative name(s):
    PDIpBy similarity
    Gene namesi
    Name:Pdia2Imported
    Synonyms:PdipBy similarity
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1916441. Pdia2.

    Subcellular locationi

    Endoplasmic reticulum lumen By similarityPROSITE-ProRule annotation

    GO - Cellular componenti

    1. endoplasmic reticulum Source: RefGenome
    2. endoplasmic reticulum lumen Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 527507Protein disulfide-isomerase A2Sequence AnalysisPRO_0000394671Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi74 ↔ 77Redox-activeBy similarityPROSITE-ProRule annotation
    Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi421 ↔ 424Redox-activeBy similarityPROSITE-ProRule annotation
    Glycosylationi518 – 5181N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiD3Z6P0.
    PRIDEiD3Z6P0.

    Expressioni

    Tissue specificityi

    Highly expressed in pancreas.1 Publication

    Gene expression databases

    BgeeiD3Z6P0.

    Interactioni

    Subunit structurei

    Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.By similarity

    Protein-protein interaction databases

    BioGridi213283. 2 interactions.
    STRINGi10090.ENSMUSP00000035584.

    Structurei

    3D structure databases

    ProteinModelPortaliD3Z6P0.
    SMRiD3Z6P0. Positions 43-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 155127Thioredoxin 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini355 – 499145Thioredoxin 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi524 – 5274Prevents secretion from ERPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the protein disulfide isomerase family.Sequence Analysis
    Contains 2 thioredoxin domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Repeat, Signal

    Phylogenomic databases

    GeneTreeiENSGT00740000115202.
    HOGENOMiHOG000162459.
    HOVERGENiHBG005920.
    KOiK09581.
    OMAiQEVPPDW.
    OrthoDBiEOG7VHSX1.
    PhylomeDBiD3Z6P0.
    TreeFamiTF106381.

    Family and domain databases

    Gene3Di3.40.30.10. 4 hits.
    InterProiIPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view]
    PfamiPF00085. Thioredoxin. 2 hits.
    [Graphical view]
    SUPFAMiSSF52833. SSF52833. 4 hits.
    TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 1 hit.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: D3Z6P0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDKQLLPVLL LLLGVSGSWG QGEEPGGPSE VLPEEPTGEE VPKEDGILVL    50
    NHRTLSLALQ EHSALMVEFY APWCGHCKEL APEYSKAAAL LAAESAVVTL 100
    AKVDGPAEPE LTKEFEVVGY PTLKFFQNGN RTNPEEYAGP KTAEGIAEWL 150
    RRRVGPSATH LEDEEGVQAL MAKWDMVVIG FFQDLQGKDM ATFLALAKDA 200
    LDMTFGFTDQ PQLFEKFGLT KDTVVLFKKF DEGRADFPVD KETGLDLGDL 250
    SRFLVIHSMH LVTEFNSQTS PKIFAAKILN HLLLFVNQTL AQHRELLTDF 300
    REAAPPFRGQ VLFVMVDVAA DNSHVLNYFG LKAEEAPTLR LINVETTKKY 350
    APTGVIAITA ASVAAFCQAV LHGEIKHYLL SQEIPPDWDQ GPVKTLVSKN 400
    FEQVAFDETK NVFVKFYAPW CSHCKEMAPA WEALAEKYKD REDIVIAELD 450
    ATANELEAFS VLGYPTLKFF PAGPDRKVID YKSTRDLETF SKFLDSGGHL 500
    PKEEPKEPAA SAPEAQANST LGPKEEL 527
    Length:527
    Mass (Da):58,316
    Last modified:April 20, 2010 - v1
    Checksum:iCF703D296B634441
    GO
    Isoform 21 Publication (identifier: D3Z6P0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         184-186: Missing.

    Show »
    Length:524
    Mass (Da):57,960
    Checksum:i4233CC4C3942C17D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei184 – 1863Missing in isoform 2. 1 PublicationVSP_039293

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC126438 Genomic DNA. No translation available.
    BC116671 mRNA. Translation: AAI16672.1.
    CCDSiCCDS37510.1. [D3Z6P0-1]
    RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
    UniGeneiMm.32631.

    Genome annotation databases

    EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
    ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
    GeneIDi69191.
    KEGGimmu:69191.
    UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
    uc012ans.1. mouse. [D3Z6P0-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC126438 Genomic DNA. No translation available.
    BC116671 mRNA. Translation: AAI16672.1 .
    CCDSi CCDS37510.1. [D3Z6P0-1 ]
    RefSeqi NP_001074539.1. NM_001081070.1. [D3Z6P0-1 ]
    UniGenei Mm.32631.

    3D structure databases

    ProteinModelPortali D3Z6P0.
    SMRi D3Z6P0. Positions 43-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 213283. 2 interactions.
    STRINGi 10090.ENSMUSP00000035584.

    Proteomic databases

    MaxQBi D3Z6P0.
    PRIDEi D3Z6P0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039113 ; ENSMUSP00000035584 ; ENSMUSG00000024184 . [D3Z6P0-1 ]
    ENSMUST00000120333 ; ENSMUSP00000114080 ; ENSMUSG00000024184 . [D3Z6P0-2 ]
    GeneIDi 69191.
    KEGGi mmu:69191.
    UCSCi uc008bdo.1. mouse. [D3Z6P0-1 ]
    uc012ans.1. mouse. [D3Z6P0-2 ]

    Organism-specific databases

    CTDi 64714.
    MGIi MGI:1916441. Pdia2.

    Phylogenomic databases

    GeneTreei ENSGT00740000115202.
    HOGENOMi HOG000162459.
    HOVERGENi HBG005920.
    KOi K09581.
    OMAi QEVPPDW.
    OrthoDBi EOG7VHSX1.
    PhylomeDBi D3Z6P0.
    TreeFami TF106381.

    Miscellaneous databases

    ChiTaRSi PDIA2. mouse.
    NextBioi 328852.
    PROi D3Z6P0.
    SOURCEi Search...

    Gene expression databases

    Bgeei D3Z6P0.

    Family and domain databases

    Gene3Di 3.40.30.10. 4 hits.
    InterProi IPR005792. Prot_disulphide_isomerase.
    IPR012336. Thioredoxin-like_fold.
    IPR017937. Thioredoxin_CS.
    IPR013766. Thioredoxin_domain.
    [Graphical view ]
    Pfami PF00085. Thioredoxin. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52833. SSF52833. 4 hits.
    TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00194. THIOREDOXIN_1. 1 hit.
    PS51352. THIOREDOXIN_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    3. "Molecular characterization of a pancreas-specific protein disulfide isomerase, PDIp."
      Desilva M.G., Notkins A.L., Lan M.S.
      DNA Cell Biol. 16:269-274(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GLYCOSYLATION.

    Entry informationi

    Entry nameiPDIA2_MOUSE
    AccessioniPrimary (citable) accession number: D3Z6P0
    Secondary accession number(s): Q14AV9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 15, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3