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D3Z6P0

- PDIA2_MOUSE

UniProt

D3Z6P0 - PDIA2_MOUSE

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Protein

Protein disulfide-isomerase A2

Gene

Pdia2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins (By similarity).By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741NucleophileBy similarity
Sitei75 – 751Contributes to redox potential valueBy similarity
Sitei76 – 761Contributes to redox potential valueBy similarity
Active sitei77 – 771NucleophileBy similarity
Active sitei421 – 4211NucleophileBy similarity
Sitei422 – 4221Contributes to redox potential valueBy similarity
Sitei423 – 4231Contributes to redox potential valueBy similarity
Active sitei424 – 4241NucleophileBy similarity
Sitei485 – 4851Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: RefGenome
  2. steroid binding Source: UniProtKB-KW

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
  2. peptidyl-proline hydroxylation Source: GOC
  3. protein folding Source: RefGenome
  4. regulation of oxidative stress-induced intrinsic apoptotic signaling pathway Source: RefGenome
  5. response to endoplasmic reticulum stress Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Keywords - Ligandi

Lipid-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A2By similarity (EC:5.3.4.1)
Alternative name(s):
PDIpBy similarity
Gene namesi
Name:Pdia2Imported
Synonyms:PdipBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1916441. Pdia2.

Subcellular locationi

Endoplasmic reticulum lumen By similarityPROSITE-ProRule annotation

GO - Cellular componenti

  1. endoplasmic reticulum Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 527507Protein disulfide-isomerase A2Sequence AnalysisPRO_0000394671Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi74 ↔ 77Redox-activeBy similarityPROSITE-ProRule annotation
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi421 ↔ 424Redox-activeBy similarityPROSITE-ProRule annotation
Glycosylationi518 – 5181N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiD3Z6P0.
PRIDEiD3Z6P0.

Expressioni

Tissue specificityi

Highly expressed in pancreas.1 Publication

Gene expression databases

BgeeiD3Z6P0.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.By similarity

Protein-protein interaction databases

BioGridi213283. 2 interactions.
STRINGi10090.ENSMUSP00000035584.

Structurei

3D structure databases

ProteinModelPortaliD3Z6P0.
SMRiD3Z6P0. Positions 43-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 155127Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini355 – 499145Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi524 – 5274Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Sequence Analysis
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00760000119201.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiD3Z6P0.
KOiK09581.
OMAiQEVPPDW.
OrthoDBiEOG7VHSX1.
PhylomeDBiD3Z6P0.
TreeFamiTF106381.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: D3Z6P0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKQLLPVLL LLLGVSGSWG QGEEPGGPSE VLPEEPTGEE VPKEDGILVL
60 70 80 90 100
NHRTLSLALQ EHSALMVEFY APWCGHCKEL APEYSKAAAL LAAESAVVTL
110 120 130 140 150
AKVDGPAEPE LTKEFEVVGY PTLKFFQNGN RTNPEEYAGP KTAEGIAEWL
160 170 180 190 200
RRRVGPSATH LEDEEGVQAL MAKWDMVVIG FFQDLQGKDM ATFLALAKDA
210 220 230 240 250
LDMTFGFTDQ PQLFEKFGLT KDTVVLFKKF DEGRADFPVD KETGLDLGDL
260 270 280 290 300
SRFLVIHSMH LVTEFNSQTS PKIFAAKILN HLLLFVNQTL AQHRELLTDF
310 320 330 340 350
REAAPPFRGQ VLFVMVDVAA DNSHVLNYFG LKAEEAPTLR LINVETTKKY
360 370 380 390 400
APTGVIAITA ASVAAFCQAV LHGEIKHYLL SQEIPPDWDQ GPVKTLVSKN
410 420 430 440 450
FEQVAFDETK NVFVKFYAPW CSHCKEMAPA WEALAEKYKD REDIVIAELD
460 470 480 490 500
ATANELEAFS VLGYPTLKFF PAGPDRKVID YKSTRDLETF SKFLDSGGHL
510 520
PKEEPKEPAA SAPEAQANST LGPKEEL
Length:527
Mass (Da):58,316
Last modified:April 20, 2010 - v1
Checksum:iCF703D296B634441
GO
Isoform 21 Publication (identifier: D3Z6P0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-186: Missing.

Show »
Length:524
Mass (Da):57,960
Checksum:i4233CC4C3942C17D
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei184 – 1863Missing in isoform 2. 1 PublicationVSP_039293

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1.
CCDSiCCDS37510.1. [D3Z6P0-1]
RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
UniGeneiMm.32631.

Genome annotation databases

EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
GeneIDi69191.
KEGGimmu:69191.
UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
uc012ans.1. mouse. [D3Z6P0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1 .
CCDSi CCDS37510.1. [D3Z6P0-1 ]
RefSeqi NP_001074539.1. NM_001081070.1. [D3Z6P0-1 ]
UniGenei Mm.32631.

3D structure databases

ProteinModelPortali D3Z6P0.
SMRi D3Z6P0. Positions 43-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 213283. 2 interactions.
STRINGi 10090.ENSMUSP00000035584.

Proteomic databases

MaxQBi D3Z6P0.
PRIDEi D3Z6P0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039113 ; ENSMUSP00000035584 ; ENSMUSG00000024184 . [D3Z6P0-1 ]
ENSMUST00000120333 ; ENSMUSP00000114080 ; ENSMUSG00000024184 . [D3Z6P0-2 ]
GeneIDi 69191.
KEGGi mmu:69191.
UCSCi uc008bdo.1. mouse. [D3Z6P0-1 ]
uc012ans.1. mouse. [D3Z6P0-2 ]

Organism-specific databases

CTDi 64714.
MGIi MGI:1916441. Pdia2.

Phylogenomic databases

GeneTreei ENSGT00760000119201.
HOGENOMi HOG000162459.
HOVERGENi HBG005920.
InParanoidi D3Z6P0.
KOi K09581.
OMAi QEVPPDW.
OrthoDBi EOG7VHSX1.
PhylomeDBi D3Z6P0.
TreeFami TF106381.

Miscellaneous databases

ChiTaRSi Pdia2. mouse.
NextBioi 328852.
PROi D3Z6P0.
SOURCEi Search...

Gene expression databases

Bgeei D3Z6P0.

Family and domain databases

Gene3Di 3.40.30.10. 4 hits.
InterProi IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  3. "Molecular characterization of a pancreas-specific protein disulfide isomerase, PDIp."
    Desilva M.G., Notkins A.L., Lan M.S.
    DNA Cell Biol. 16:269-274(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GLYCOSYLATION.

Entry informationi

Entry nameiPDIA2_MOUSE
AccessioniPrimary (citable) accession number: D3Z6P0
Secondary accession number(s): Q14AV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 20, 2010
Last modified: November 26, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3