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Protein

Protein disulfide-isomerase A2

Gene

Pdia2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins (By similarity).By similarity

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74NucleophileBy similarity1
Sitei75Contributes to redox potential valueBy similarity1
Sitei76Contributes to redox potential valueBy similarity1
Active sitei77NucleophileBy similarity1
Active sitei421NucleophileBy similarity1
Sitei422Contributes to redox potential valueBy similarity1
Sitei423Contributes to redox potential valueBy similarity1
Active sitei424NucleophileBy similarity1
Sitei485Lowers pKa of C-terminal Cys of second active siteBy similarity1

GO - Molecular functioni

  • protein disulfide isomerase activity Source: MGI
  • steroid binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Keywords - Ligandi

Lipid-binding, Steroid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase A2By similarity (EC:5.3.4.1)
Alternative name(s):
PDIpBy similarity
Gene namesi
Name:Pdia2Imported
Synonyms:PdipBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1916441. Pdia2.

Subcellular locationi

  • Endoplasmic reticulum lumen PROSITE-ProRule annotationBy similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000039467121 – 527Protein disulfide-isomerase A2Sequence analysisAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi74 ↔ 77Redox-activePROSITE-ProRule annotationBy similarity
Glycosylationi130N-linked (GlcNAc...)Sequence analysis1
Glycosylationi287N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi421 ↔ 424Redox-activePROSITE-ProRule annotationBy similarity
Glycosylationi518N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiD3Z6P0.
PaxDbiD3Z6P0.
PeptideAtlasiD3Z6P0.
PRIDEiD3Z6P0.

PTM databases

iPTMnetiD3Z6P0.
PhosphoSitePlusiD3Z6P0.

Expressioni

Tissue specificityi

Highly expressed in pancreas.1 Publication

Gene expression databases

BgeeiENSMUSG00000024184.
ExpressionAtlasiD3Z6P0. baseline and differential.
GenevisibleiD3Z6P0. MM.

Interactioni

Subunit structurei

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.By similarity

Protein-protein interaction databases

BioGridi213283. 2 interactors.
STRINGi10090.ENSMUSP00000035584.

Structurei

3D structure databases

ProteinModelPortaliD3Z6P0.
SMRiD3Z6P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 155Thioredoxin 1PROSITE-ProRule annotationAdd BLAST127
Domaini355 – 499Thioredoxin 2PROSITE-ProRule annotationAdd BLAST145

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi524 – 527Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Sequence analysis
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiD3Z6P0.
KOiK09581.
OMAiQEVPPDW.
OrthoDBiEOG091G08WM.
PhylomeDBiD3Z6P0.
TreeFamiTF106381.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: D3Z6P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKQLLPVLL LLLGVSGSWG QGEEPGGPSE VLPEEPTGEE VPKEDGILVL
60 70 80 90 100
NHRTLSLALQ EHSALMVEFY APWCGHCKEL APEYSKAAAL LAAESAVVTL
110 120 130 140 150
AKVDGPAEPE LTKEFEVVGY PTLKFFQNGN RTNPEEYAGP KTAEGIAEWL
160 170 180 190 200
RRRVGPSATH LEDEEGVQAL MAKWDMVVIG FFQDLQGKDM ATFLALAKDA
210 220 230 240 250
LDMTFGFTDQ PQLFEKFGLT KDTVVLFKKF DEGRADFPVD KETGLDLGDL
260 270 280 290 300
SRFLVIHSMH LVTEFNSQTS PKIFAAKILN HLLLFVNQTL AQHRELLTDF
310 320 330 340 350
REAAPPFRGQ VLFVMVDVAA DNSHVLNYFG LKAEEAPTLR LINVETTKKY
360 370 380 390 400
APTGVIAITA ASVAAFCQAV LHGEIKHYLL SQEIPPDWDQ GPVKTLVSKN
410 420 430 440 450
FEQVAFDETK NVFVKFYAPW CSHCKEMAPA WEALAEKYKD REDIVIAELD
460 470 480 490 500
ATANELEAFS VLGYPTLKFF PAGPDRKVID YKSTRDLETF SKFLDSGGHL
510 520
PKEEPKEPAA SAPEAQANST LGPKEEL
Length:527
Mass (Da):58,316
Last modified:April 20, 2010 - v1
Checksum:iCF703D296B634441
GO
Isoform 21 Publication (identifier: D3Z6P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     184-186: Missing.

Show »
Length:524
Mass (Da):57,960
Checksum:i4233CC4C3942C17D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_039293184 – 186Missing in isoform 2. 1 Publication3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1.
CCDSiCCDS37510.1. [D3Z6P0-1]
RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
UniGeneiMm.32631.

Genome annotation databases

EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
GeneIDi69191.
KEGGimmu:69191.
UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
uc012ans.1. mouse. [D3Z6P0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC126438 Genomic DNA. No translation available.
BC116671 mRNA. Translation: AAI16672.1.
CCDSiCCDS37510.1. [D3Z6P0-1]
RefSeqiNP_001074539.1. NM_001081070.1. [D3Z6P0-1]
UniGeneiMm.32631.

3D structure databases

ProteinModelPortaliD3Z6P0.
SMRiD3Z6P0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213283. 2 interactors.
STRINGi10090.ENSMUSP00000035584.

PTM databases

iPTMnetiD3Z6P0.
PhosphoSitePlusiD3Z6P0.

Proteomic databases

MaxQBiD3Z6P0.
PaxDbiD3Z6P0.
PeptideAtlasiD3Z6P0.
PRIDEiD3Z6P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039113; ENSMUSP00000035584; ENSMUSG00000024184. [D3Z6P0-1]
ENSMUST00000120333; ENSMUSP00000114080; ENSMUSG00000024184. [D3Z6P0-2]
GeneIDi69191.
KEGGimmu:69191.
UCSCiuc008bdo.1. mouse. [D3Z6P0-1]
uc012ans.1. mouse. [D3Z6P0-2]

Organism-specific databases

CTDi64714.
MGIiMGI:1916441. Pdia2.

Phylogenomic databases

eggNOGiKOG0190. Eukaryota.
COG0526. LUCA.
GeneTreeiENSGT00860000133691.
HOGENOMiHOG000162459.
HOVERGENiHBG005920.
InParanoidiD3Z6P0.
KOiK09581.
OMAiQEVPPDW.
OrthoDBiEOG091G08WM.
PhylomeDBiD3Z6P0.
TreeFamiTF106381.

Miscellaneous databases

ChiTaRSiPdia2. mouse.
PROiD3Z6P0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024184.
ExpressionAtlasiD3Z6P0. baseline and differential.
GenevisibleiD3Z6P0. MM.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDIA2_MOUSE
AccessioniPrimary (citable) accession number: D3Z6P0
Secondary accession number(s): Q14AV9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 20, 2010
Last modified: November 30, 2016
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.