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D3Z6I3

- D3Z6I3_MOUSE

UniProt

D3Z6I3 - D3Z6I3_MOUSE

Protein

Lipoyl synthase, mitochondrial

Gene

Lias

Organism
Mus musculus (Mouse)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi26 – 261Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi32 – 321Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi52 – 521Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi56 – 561Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: MGI
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. inflammatory response Source: MGI
    2. lipoate biosynthetic process Source: MGI
    3. neural tube closure Source: MGI
    4. protein lipoylation Source: UniProtKB-HAMAP
    5. response to lipopolysaccharide Source: MGI
    6. response to oxidative stress Source: MGI

    Keywords - Molecular functioni

    TransferaseUniRule annotation

    Keywords - Ligandi

    4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LiasUniRule annotationImported
    OrganismiMus musculus (Mouse)Imported
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1934604. Lias.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: MGI

    Keywords - Cellular componenti

    MitochondrionUniRule annotation

    Expressioni

    Gene expression databases

    ArrayExpressiD3Z6I3.
    BgeeiD3Z6I3.

    Structurei

    3D structure databases

    ProteinModelPortaliD3Z6I3.
    SMRiD3Z6I3. Positions 48-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptideUniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    D3Z6I3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKNYNKLKN TLRNLSLHTV CEEARCPNIG ECWGGGEYAT ATATIMLMGD    50
    TCTRGCRFCS VKTARNPPPL DANEPDNTAK AIAEWGLDYV VLTSVDRDDV 100
    ADGGAEHIAK TVSCLKERNP KILVECLTPD FRGDLRAVEK VALSGLDVYA 150
    HNVETVPELQ RKVRDPRANF DQSLRVLRHA KEVQPDVVSK TSIMLGLGET 200
    DEQVYATLKA LRAADVDCLT LGQYMQPTKR HLKVEEYVTP EKFKYWEKVG 250
    NELGFLYTAS GPLVRSSYKA GEFFLKNLVA RRKTKASKV 289
    Length:289
    Mass (Da):32,174
    Last modified:April 20, 2010 - v1
    Checksum:iB6888EC642EBDD0B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC136513 Genomic DNA. No translation available.
    RefSeqiXP_006504257.1. XM_006504194.1.
    UniGeneiMm.195776.

    Genome annotation databases

    EnsembliENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199.
    GeneIDi79464.
    KEGGimmu:79464.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC136513 Genomic DNA. No translation available.
    RefSeqi XP_006504257.1. XM_006504194.1.
    UniGenei Mm.195776.

    3D structure databases

    ProteinModelPortali D3Z6I3.
    SMRi D3Z6I3. Positions 48-229.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000122026 ; ENSMUSP00000113228 ; ENSMUSG00000029199 .
    GeneIDi 79464.
    KEGGi mmu:79464.

    Organism-specific databases

    CTDi 11019.
    MGIi MGI:1934604. Lias.

    Phylogenomic databases

    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 35551465.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi D3Z6I3.
    Bgeei D3Z6I3.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6JImported.
    2. Ensembl
      Submitted (MAY-2011) to UniProtKB
      Cited for: IDENTIFICATION.
      Strain: C57BL/6JImported.

    Entry informationi

    Entry nameiD3Z6I3_MOUSE
    AccessioniPrimary (citable) accession number: D3Z6I3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Caution

    The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3