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D3Z6I3 (D3Z6I3_MOUSE) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase, mitochondrial HAMAP-Rule MF_03123

EC=2.8.1.8 HAMAP-Rule MF_03123
Alternative name(s):
Lipoate synthase HAMAP-Rule MF_03123
Lipoic acid synthase HAMAP-Rule MF_03123
Gene names
Name:Lias HAMAP-Rule MF_03123 Ensembl ENSMUSP00000113228 MGI 1934604
OrganismMus musculus (Mouse) [Reference proteome] Ensembl ENSMUSP00000113228
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_03123

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123

Subcellular location

Mitochondrion By similarity HAMAP-Rule MF_03123.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_03123

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Ensembl ENSMUSP00000113228

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding211Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123
Metal binding261Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123
Metal binding321Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123
Metal binding521Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123
Metal binding561Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123
Metal binding591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123

Sequences

Sequence LengthMass (Da)Tools
D3Z6I3 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: B6888EC642EBDD0B

FASTA28932,174
        10         20         30         40         50         60 
MGKNYNKLKN TLRNLSLHTV CEEARCPNIG ECWGGGEYAT ATATIMLMGD TCTRGCRFCS 

        70         80         90        100        110        120 
VKTARNPPPL DANEPDNTAK AIAEWGLDYV VLTSVDRDDV ADGGAEHIAK TVSCLKERNP 

       130        140        150        160        170        180 
KILVECLTPD FRGDLRAVEK VALSGLDVYA HNVETVPELQ RKVRDPRANF DQSLRVLRHA 

       190        200        210        220        230        240 
KEVQPDVVSK TSIMLGLGET DEQVYATLKA LRAADVDCLT LGQYMQPTKR HLKVEEYVTP 

       250        260        270        280 
EKFKYWEKVG NELGFLYTAS GPLVRSSYKA GEFFLKNLVA RRKTKASKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC136513 Genomic DNA. No translation available.
RefSeqXP_006504257.1. XM_006504194.1.
UniGeneMm.195776.

3D structure databases

ProteinModelPortalD3Z6I3.
SMRD3Z6I3. Positions 48-229.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199.
GeneID79464.
KEGGmmu:79464.

Organism-specific databases

CTD11019.
MGIMGI:1934604. Lias.

Phylogenomic databases

GeneTreeENSGT00390000006234.
HOGENOMHOG000235998.
KOK03644.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Gene expression databases

ArrayExpressD3Z6I3.
BgeeD3Z6I3.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Other

NextBio35551465.
SOURCESearch...

Entry information

Entry nameD3Z6I3_MOUSE
AccessionPrimary (citable) accession number: D3Z6I3
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: July 9, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)