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D3Z6I3

- D3Z6I3_MOUSE

UniProt

D3Z6I3 - D3Z6I3_MOUSE

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Protein

Lipoyl synthase, mitochondrial

Gene

Lias

Organism
Mus musculus (Mouse)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi21 – 211Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi26 – 261Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi32 – 321Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi52 – 521Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi56 – 561Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. inflammatory response Source: MGI
  2. lipoate biosynthetic process Source: MGI
  3. neural tube closure Source: MGI
  4. protein lipoylation Source: UniProtKB-HAMAP
  5. response to lipopolysaccharide Source: MGI
  6. response to oxidative stress Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LiasUniRule annotationImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1934604. Lias.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

MitochondrionUniRule annotation

Expressioni

Gene expression databases

BgeeiD3Z6I3.
ExpressionAtlasiD3Z6I3. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliD3Z6I3.
SMRiD3Z6I3. Positions 48-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptideUniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000006234.
HOGENOMiHOG000235998.
KOiK03644.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

D3Z6I3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKNYNKLKN TLRNLSLHTV CEEARCPNIG ECWGGGEYAT ATATIMLMGD
60 70 80 90 100
TCTRGCRFCS VKTARNPPPL DANEPDNTAK AIAEWGLDYV VLTSVDRDDV
110 120 130 140 150
ADGGAEHIAK TVSCLKERNP KILVECLTPD FRGDLRAVEK VALSGLDVYA
160 170 180 190 200
HNVETVPELQ RKVRDPRANF DQSLRVLRHA KEVQPDVVSK TSIMLGLGET
210 220 230 240 250
DEQVYATLKA LRAADVDCLT LGQYMQPTKR HLKVEEYVTP EKFKYWEKVG
260 270 280
NELGFLYTAS GPLVRSSYKA GEFFLKNLVA RRKTKASKV
Length:289
Mass (Da):32,174
Last modified:April 20, 2010 - v1
Checksum:iB6888EC642EBDD0B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC136513 Genomic DNA. No translation available.
RefSeqiXP_006504257.1. XM_006504194.1.
UniGeneiMm.195776.

Genome annotation databases

EnsembliENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199.
GeneIDi79464.
KEGGimmu:79464.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC136513 Genomic DNA. No translation available.
RefSeqi XP_006504257.1. XM_006504194.1.
UniGenei Mm.195776.

3D structure databases

ProteinModelPortali D3Z6I3.
SMRi D3Z6I3. Positions 48-229.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000122026 ; ENSMUSP00000113228 ; ENSMUSG00000029199 .
GeneIDi 79464.
KEGGi mmu:79464.

Organism-specific databases

CTDi 11019.
MGIi MGI:1934604. Lias.

Phylogenomic databases

GeneTreei ENSGT00390000006234.
HOGENOMi HOG000235998.
KOi K03644.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Miscellaneous databases

NextBioi 35551465.
SOURCEi Search...

Gene expression databases

Bgeei D3Z6I3.
ExpressionAtlasi D3Z6I3. baseline and differential.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6JImported.
  2. Ensembl
    Submitted (MAY-2011) to UniProtKB
    Cited for: IDENTIFICATION.
    Strain: C57BL/6JImported.

Entry informationi

Entry nameiD3Z6I3_MOUSE
AccessioniPrimary (citable) accession number: D3Z6I3
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3