Lipoyl synthase, mitochondrial - Mus musculus (Mouse)

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D3Z6I3 (D3Z6I3_MOUSE) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 30. History...

Clusters with 100%, 90%, 50% identity |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info Customize order

Names and origin

Protein names

Recommended name:
Lipoyl synthase, mitochondrial HAMAP-Rule MF_03123
EC=2.8.1.8 HAMAP-Rule MF_03123

Alternative name(s):
Lipoate synthase HAMAP-Rule MF_03123
Lipoic acid synthase HAMAP-Rule MF_03123

Gene names

Name:	Lias Ensembl ENSMUSP00000113228 MGI 1934604
Synonyms:	LIAS HAMAP-Rule MF_03123

Organism

Mus musculus (Mouse) [Reference proteome] Ensembl ENSMUSP00000113228

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03123
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03123
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_03123
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03123
Subcellular location	Mitochondrion By similarity HAMAP-Rule MF_03123.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_03123
Caution	The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. Ensembl ENSMUSP00000113228

Ontologies

Keywords
Cellular component	Mitochondrion HAMAP-Rule MF_03123
Domain	Transit peptide HAMAP-Rule MF_03123
Ligand	4Fe-4S HAMAP-Rule MF_03123 Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine HAMAP-Rule MF_03123
Molecular function	Transferase HAMAP-Rule MF_03123
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inflammatory response Inferred from mutant phenotype PubMed 18845616. Source: MGI neural tube closure Inferred from mutant phenotype PubMed 15755804. Source: MGI protein lipoylation Inferred from electronic annotation. Source: HAMAP response to lipopolysaccharide Inferred from mutant phenotype PubMed 18845616. Source: MGI response to oxidative stress Inferred from mutant phenotype PubMed 18845616. Source: MGI
Cellular_component	mitochondrion Inferred from direct assay PubMed 11389890 PubMed 14651853 PubMed 18614015. Source: MGI
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from genetic interaction PubMed 11389890. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123

Metal binding

Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_03123

Metal binding

Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123

Metal binding

Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123

Metal binding

Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_03123

Sequences

Sequence

Length

Mass (Da)

Tools

D3Z6I3 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: B6888EC642EBDD0B
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FASTA

289

32,174

        10         20         30         40         50         60 
MGKNYNKLKN TLRNLSLHTV CEEARCPNIG ECWGGGEYAT ATATIMLMGD TCTRGCRFCS 

        70         80         90        100        110        120 
VKTARNPPPL DANEPDNTAK AIAEWGLDYV VLTSVDRDDV ADGGAEHIAK TVSCLKERNP 

       130        140        150        160        170        180 
KILVECLTPD FRGDLRAVEK VALSGLDVYA HNVETVPELQ RKVRDPRANF DQSLRVLRHA 

       190        200        210        220        230        240 
KEVQPDVVSK TSIMLGLGET DEQVYATLKA LRAADVDCLT LGQYMQPTKR HLKVEEYVTP 

       250        260        270        280 
EKFKYWEKVG NELGFLYTAS GPLVRSSYKA GEFFLKNLVA RRKTKASKV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J Ensembl ENSMUSP00000113228.
[2]	Ensembl Submitted (MAY-2011) to UniProtKB Cited for: IDENTIFICATION. Strain: C57BL/6J Ensembl ENSMUSP00000113228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC136513 Genomic DNA. No translation available.
IPI	IPI00882331.
3D structure databases
ProteinModelPortal	D3Z6I3.
SMR	D3Z6I3. Positions 46-225.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000122026; ENSMUSP00000113228; ENSMUSG00000029199.
UCSC	uc008xnr.1. mouse.
Organism-specific databases
MGI	MGI:1934604. Lias.
Phylogenomic databases
GeneTree	ENSGT00390000006234.
HOGENOM	HOG000235998.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Gene expression databases
ArrayExpress	D3Z6I3.
Bgee	D3Z6I3.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...
Other
NextBio	35551465.
SOURCE	Search...

Entry information

Entry name

D3Z6I3_MOUSE

Accession

Primary (citable) accession number: D3Z6I3

Entry history

Integrated into UniProtKB/TrEMBL:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information