D3Z5L9 (WISP3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
WNT1-inducible-signaling pathway protein 3
Short name=WISP-3

Alternative name(s):
CCN family member 6

Gene names

Name:	Wisp3
Synonyms:	Ccn6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Appears to be required for normal postnatal skeletal growth and cartilage homeostasis By similarity.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 354	331	WNT1-inducible-signaling pathway protein 3 By similarity		PRO_0000415855
Regions
	Domain	40 – 117	78	IGFBP N-terminal
	Domain	208 – 253	46	TSP type-1
	Domain	268 – 342	75	CTCK
Amino acid modifications
	Glycosylation	308	1	N-linked (GlcNAc...) Potential
	Disulfide bond	209 ↔ 238		By similarity
	Disulfide bond	219 ↔ 223		By similarity
	Disulfide bond	247 ↔ 252		By similarity
	Disulfide bond	268 ↔ 305		By similarity
	Disulfide bond	285 ↔ 319		By similarity
	Disulfide bond	296 ↔ 335		By similarity
	Disulfide bond	299 ↔ 337		By similarity

Sequences

	Sequence		Length	Mass (Da)
	D3Z5L9 [UniParc]. Last modified April 5, 2011. Version 2. Checksum: F45C5CD8896D1647	FASTA	354	39,379
	10 20 30 40 50 60 MRRLLFCTLL MTGLTQLCCR TQGSAPQDST PGGRPGAALE VYQRTEVCRW PCRCPPQRPT 70 80 90 100 110 120 CPPGVSLVRD GCGCCKVCAK QPGDTCNEAE ICDPHKGLYC DYSGDTPRYE TGVCAYLVAV 130 140 150 160 170 180 GCEFNRVYYQ NGQVFQPHPL FSCLCVSGAI GCTPLFIPKL AGSNCSAAKG RRKTDPPNCG 190 200 210 220 230 240 RGTLQQQNSA SYKTMSAYRN LPLTWRKKCL VQATKWTPCS RTCGMGISNR VTNDNANCEM 250 260 270 280 290 300 RKERRLCYIQ PCSRNTSQAV KIPRGETCQP TFQLPKAEKF VFSGCSSTQS YRPTFCGICL 310 320 330 340 350 DKRCCVPNKS KMITVRFDCP SEGSFKWQML WVTSCVCQRD CREPGDIFSE LRIL

References

[1]

"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC153958 Genomic DNA. No translation available.
IPI	IPI00624914.
RefSeq	NP_001120848.1. NM_001127376.1.
UniGene	Mm.208111.
3D structure databases
ProteinModelPortal	D3Z5L9.
SMR	D3Z5L9. Positions 213-252.
ModBase	Search...
Proteomic databases
PRIDE	D3Z5L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000076713; ENSMUSP00000076003; ENSMUSG00000062074.
GeneID	327743.
KEGG	mmu:327743.
UCSC	uc011xcu.1. mouse.
Organism-specific databases
CTD	8838.
MGI	MGI:2685581. Wisp3.
Phylogenomic databases
GeneTree	ENSGT00550000074368.
HOGENOM	HOG000231462.
OMA	ICNEADL.
OrthoDB	EOG4PNXH0.
Gene expression databases
Bgee	D3Z5L9.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	397926.
SOURCE	Search...

Entry information

Entry name

WISP3_MOUSE

Accession

Primary (citable) accession number: D3Z5L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 22, 2012
Last sequence update:	April 5, 2011
Last modified:	May 1, 2013
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program