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D3Z3K2 (CIP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
E3 ubiquitin-protein ligase CCNB1IP1

EC=6.3.2.-
Alternative name(s):
Cyclin-B1-interacting protein 1
Gene names
Name:Ccnb1ip1
Synonyms:Gm288, Hei10, Mei4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length276 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over. Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis. Ref.3 Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with CCNB1, UBE2L3 and NF2 By similarity.

Subcellular location

Nucleus. Chromosome. Note: Associates to the synaptonemal complex. Ref.5

Tissue specificity

Expressed predominantly in the testes and 17 day embryos (corresponding to prophase I in females). Weakly or not expressed in other tissues. Ref.3

Developmental stage

In spermatocytes, synaptonemal complex-associated Ccnb1ip1 foci are detected by early pachynema and their number peaks during midpachynema. In late-pachytene nuclei, Ccnb1ip1 foci number descrease. At the onset of diplonema, foci are no longer detected (at protein level). Ref.4 Ref.5

Post-translational modification

Ubiquitinated; autoubiquitinated By similarity.

Phosphorylated by CDK1 on serine or threonine residues (in vitro) By similarity.

Disruption phenotype

Both male and female mice are sterile. Males do not make sperm and have much smaller testes, a characteristic of mutants with meiotic defects. Early stages of meiosis occur normally and full synapsis of homologous chromosomes (homologs) is achieved. However, crossover-specific recombination complexes do not assemble and crossing-overs fail. In spermatocytes, chromosomes fail to congress properly at the metaphase plate, leading to arrest and apoptosis before the first meiotic division. Mutant oocytes have a similar chromosomal phenotype but can undergo meiotic divisions and fertilization before arresting. During late meiotic prophase males, absence of Cdk2 and mismatch repair protein association from chromosome cores is correlated with the premature separation of bivalents at diplonema owing to lack of chiasmata. Ref.3 Ref.5

Sequence similarities

Contains 1 RING-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 276276E3 ubiquitin-protein ligase CCNB1IP1
PRO_0000425901

Regions

Zinc finger10 – 5243RING-type; atypical
Coiled coil146 – 18237 Potential

Experimental info

Mutagenesis77 – 10024Missing in mei4; Sterility in both sexes due to meiotic defects.

Sequences

Sequence LengthMass (Da)Tools
D3Z3K2 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 8677CFEC187C1465

FASTA27631,386
        10         20         30         40         50         60 
MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA CNSTLSGKLD 

        70         80         90        100        110        120 
IVRTELSPSE EYKAMVLAGL RPEVVLDISS RALAFWTYQV HQERLYQEYN FSKAENHLKQ 

       130        140        150        160        170        180 
MEKMYMQQIQ SKNIELTSMK GEVISMKKVL EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS 

       190        200        210        220        230        240 
LRLRNITIAS QEGSLEPGMI PQSGVFGFPP GNNSKFSLDH IPVGNQGGGD EDVQFRPFFV 

       250        260        270 
CSPTAPEPIN NFFSFASPSH EAEQQVCSRA FKAKRI 

« Hide

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Mutation in mouse hei10, an e3 ubiquitin ligase, disrupts meiotic crossing over."
Ward J.O., Reinholdt L.G., Motley W.W., Niswander L.M., Deacon D.C., Griffin L.B., Langlais K.K., Backus V.L., Schimenti K.J., O'Brien M.J., Eppig J.J., Schimenti J.C.
PLoS Genet. 3:E139-E139(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF 77-LEU--VAL-110.
[4]"Evidence implicating CCNB1IP1, a RING domain-containing protein required for meiotic crossing over in mice, as an E3 SUMO ligase."
Strong E.R., Schimenti J.C.
Genes (Basel) 1:440-451(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[5]"Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination."
Qiao H., Prasada Rao H.B., Yang Y., Fong J.H., Cloutier J.M., Deacon D.C., Nagel K.E., Swartz R.K., Strong E., Holloway J.K., Cohen P.E., Schimenti J., Ward J., Hunter N.
Nat. Genet. 46:194-199(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC027184 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20823.1.
RefSeqNP_001104589.1. NM_001111119.1.
XP_006519036.1. XM_006518973.1.
UniGeneMm.343880.

3D structure databases

SMRD3Z3K2. Positions 5-61.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000095932; ENSMUSP00000093622; ENSMUSG00000071470.
GeneID239083.
KEGGmmu:239083.
UCSCuc011zjw.1. mouse.

Organism-specific databases

CTD57820.
MGIMGI:2685134. Ccnb1ip1.

Phylogenomic databases

GeneTreeENSGT00390000002849.
HOGENOMHOG000111677.
KOK10639.
OMAIANHEGT.
OrthoDBEOG76MK99.
PhylomeDBD3Z3K2.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

InterProIPR001841. Znf_RING.
[Graphical view]
PfamPF14634. zf-RING_5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio383975.
PROD3Z3K2.
SOURCESearch...

Entry information

Entry nameCIP1_MOUSE
AccessionPrimary (citable) accession number: D3Z3K2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: April 20, 2010
Last modified: April 16, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot