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D3Z3K2

- CIP1_MOUSE

UniProt

D3Z3K2 - CIP1_MOUSE

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Protein

E3 ubiquitin-protein ligase CCNB1IP1

Gene

Ccnb1ip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over. Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.2 Publications

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 5243RING-type; atypicalAdd
BLAST

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. blastocyst formation Source: MGI
  2. chiasma assembly Source: UniProtKB
  3. protein ubiquitination Source: UniProtKB
  4. reciprocal meiotic recombination Source: UniProtKB
  5. spermatid development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Meiosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CCNB1IP1 (EC:6.3.2.-)
Alternative name(s):
Cyclin-B1-interacting protein 1
Gene namesi
Name:Ccnb1ip1
Synonyms:Gm288, Hei10, Mei4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:2685134. Ccnb1ip1.

Subcellular locationi

Nucleus 1 Publication. Chromosome 1 Publication
Note: Associates to the synaptonemal complex.

GO - Cellular componenti

  1. chromosome Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Both male and female mice are sterile. Males do not make sperm and have much smaller testes, a characteristic of mutants with meiotic defects. Early stages of meiosis occur normally and full synapsis of homologous chromosomes (homologs) is achieved. However, crossover-specific recombination complexes do not assemble and crossing-overs fail. In spermatocytes, chromosomes fail to congress properly at the metaphase plate, leading to arrest and apoptosis before the first meiotic division. Mutant oocytes have a similar chromosomal phenotype but can undergo meiotic divisions and fertilization before arresting. During late meiotic prophase males, absence of Cdk2 and mismatch repair protein association from chromosome cores is correlated with the premature separation of bivalents at diplonema owing to lack of chiasmata.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 10024Missing in mei4; Sterility in both sexes due to meiotic defects. Add
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276E3 ubiquitin-protein ligase CCNB1IP1PRO_0000425901Add
BLAST

Post-translational modificationi

Ubiquitinated; autoubiquitinated.By similarity
Phosphorylated by CDK1 on serine or threonine residues (in vitro).By similarity

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Expressed predominantly in the testes and 17 day embryos (corresponding to prophase I in females). Weakly or not expressed in other tissues.1 Publication

Developmental stagei

In spermatocytes, synaptonemal complex-associated Ccnb1ip1 foci are detected by early pachynema and their number peaks during midpachynema. In late-pachytene nuclei, Ccnb1ip1 foci number descrease. At the onset of diplonema, foci are no longer detected (at protein level).2 Publications

Interactioni

Subunit structurei

Interacts with CCNB1, UBE2L3 and NF2.By similarity

Structurei

3D structure databases

ProteinModelPortaliD3Z3K2.
SMRiD3Z3K2. Positions 3-56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili146 – 18237Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 5243RING-type; atypicalAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00390000002849.
HOGENOMiHOG000111677.
InParanoidiD3Z3K2.
KOiK10639.
OMAiIANHEGT.
OrthoDBiEOG76MK99.
PhylomeDBiD3Z3K2.

Family and domain databases

InterProiIPR001841. Znf_RING.
[Graphical view]
PfamiPF14634. zf-RING_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3Z3K2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA
60 70 80 90 100
CNSTLSGKLD IVRTELSPSE EYKAMVLAGL RPEVVLDISS RALAFWTYQV
110 120 130 140 150
HQERLYQEYN FSKAENHLKQ MEKMYMQQIQ SKNIELTSMK GEVISMKKVL
160 170 180 190 200
EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS LRLRNITIAS QEGSLEPGMI
210 220 230 240 250
PQSGVFGFPP GNNSKFSLDH IPVGNQGGGD EDVQFRPFFV CSPTAPEPIN
260 270
NFFSFASPSH EAEQQVCSRA FKAKRI
Length:276
Mass (Da):31,386
Last modified:April 20, 2010 - v1
Checksum:i8677CFEC187C1465
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC027184 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20823.1.
CCDSiCCDS49477.1.
RefSeqiNP_001104589.1. NM_001111119.1.
XP_006519036.1. XM_006518973.1.
UniGeneiMm.343880.

Genome annotation databases

EnsembliENSMUST00000095932; ENSMUSP00000093622; ENSMUSG00000071470.
GeneIDi239083.
KEGGimmu:239083.
UCSCiuc011zjw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC027184 Genomic DNA. No translation available.
CH466605 Genomic DNA. Translation: EDL20823.1 .
CCDSi CCDS49477.1.
RefSeqi NP_001104589.1. NM_001111119.1.
XP_006519036.1. XM_006518973.1.
UniGenei Mm.343880.

3D structure databases

ProteinModelPortali D3Z3K2.
SMRi D3Z3K2. Positions 3-56.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000095932 ; ENSMUSP00000093622 ; ENSMUSG00000071470 .
GeneIDi 239083.
KEGGi mmu:239083.
UCSCi uc011zjw.1. mouse.

Organism-specific databases

CTDi 57820.
MGIi MGI:2685134. Ccnb1ip1.

Phylogenomic databases

GeneTreei ENSGT00390000002849.
HOGENOMi HOG000111677.
InParanoidi D3Z3K2.
KOi K10639.
OMAi IANHEGT.
OrthoDBi EOG76MK99.
PhylomeDBi D3Z3K2.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Miscellaneous databases

NextBioi 383975.
PROi D3Z3K2.
SOURCEi Search...

Family and domain databases

InterProi IPR001841. Znf_RING.
[Graphical view ]
Pfami PF14634. zf-RING_5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF 77-LEU--VAL-110.
  4. "Evidence implicating CCNB1IP1, a RING domain-containing protein required for meiotic crossing over in mice, as an E3 SUMO ligase."
    Strong E.R., Schimenti J.C.
    Genes (Basel) 1:440-451(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  5. "Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination."
    Qiao H., Prasada Rao H.B., Yang Y., Fong J.H., Cloutier J.M., Deacon D.C., Nagel K.E., Swartz R.K., Strong E., Holloway J.K., Cohen P.E., Schimenti J., Ward J., Hunter N.
    Nat. Genet. 46:194-199(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiCIP1_MOUSE
AccessioniPrimary (citable) accession number: D3Z3K2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: April 20, 2010
Last modified: October 29, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3