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D3Z3K2

- CIP1_MOUSE

UniProt

D3Z3K2 - CIP1_MOUSE

Protein

E3 ubiquitin-protein ligase CCNB1IP1

Gene

Ccnb1ip1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Ubiquitin E3 ligase that acts as a limiting factor for crossing-over during meiosis: required during zygonema to limit the colocalization of RNF212 with MutS-gamma-associated recombination sites and thereby establish early differentiation of crossover and non-crossover sites. Later, it is directed by MutL-gamma to stably accumulate at designated crossover sites. Probably promotes the dissociation of RNF212 and MutS-gamma to allow the progression of recombination and the implementation of the final steps of crossing over. Modulates cyclin-B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis.2 Publications

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri10 – 5243RING-type; atypicalAdd
    BLAST

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. blastocyst formation Source: MGI
    2. chiasma assembly Source: UniProtKB
    3. protein ubiquitination Source: UniProtKB
    4. reciprocal meiotic recombination Source: UniProtKB
    5. spermatid development Source: MGI

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Meiosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    E3 ubiquitin-protein ligase CCNB1IP1 (EC:6.3.2.-)
    Alternative name(s):
    Cyclin-B1-interacting protein 1
    Gene namesi
    Name:Ccnb1ip1
    Synonyms:Gm288, Hei10, Mei4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:2685134. Ccnb1ip1.

    Subcellular locationi

    Nucleus 1 Publication. Chromosome 1 Publication
    Note: Associates to the synaptonemal complex.

    GO - Cellular componenti

    1. chromosome Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Both male and female mice are sterile. Males do not make sperm and have much smaller testes, a characteristic of mutants with meiotic defects. Early stages of meiosis occur normally and full synapsis of homologous chromosomes (homologs) is achieved. However, crossover-specific recombination complexes do not assemble and crossing-overs fail. In spermatocytes, chromosomes fail to congress properly at the metaphase plate, leading to arrest and apoptosis before the first meiotic division. Mutant oocytes have a similar chromosomal phenotype but can undergo meiotic divisions and fertilization before arresting. During late meiotic prophase males, absence of Cdk2 and mismatch repair protein association from chromosome cores is correlated with the premature separation of bivalents at diplonema owing to lack of chiasmata.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi77 – 10024Missing in mei4; Sterility in both sexes due to meiotic defects. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 276276E3 ubiquitin-protein ligase CCNB1IP1PRO_0000425901Add
    BLAST

    Post-translational modificationi

    Ubiquitinated; autoubiquitinated.By similarity
    Phosphorylated by CDK1 on serine or threonine residues (in vitro).By similarity

    Keywords - PTMi

    Ubl conjugation

    Expressioni

    Tissue specificityi

    Expressed predominantly in the testes and 17 day embryos (corresponding to prophase I in females). Weakly or not expressed in other tissues.1 Publication

    Developmental stagei

    In spermatocytes, synaptonemal complex-associated Ccnb1ip1 foci are detected by early pachynema and their number peaks during midpachynema. In late-pachytene nuclei, Ccnb1ip1 foci number descrease. At the onset of diplonema, foci are no longer detected (at protein level).2 Publications

    Interactioni

    Subunit structurei

    Interacts with CCNB1, UBE2L3 and NF2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliD3Z3K2.
    SMRiD3Z3K2. Positions 5-58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili146 – 18237Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RING-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri10 – 5243RING-type; atypicalAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Zinc-finger

    Phylogenomic databases

    GeneTreeiENSGT00390000002849.
    HOGENOMiHOG000111677.
    KOiK10639.
    OMAiIANHEGT.
    OrthoDBiEOG76MK99.
    PhylomeDBiD3Z3K2.

    Family and domain databases

    InterProiIPR001841. Znf_RING.
    [Graphical view]
    PfamiPF14634. zf-RING_5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3Z3K2-1 [UniParc]FASTAAdd to Basket

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    MSLCEDMLLC NYRKCRIKLS GYAWVTACSH IFCDQHGSGE FSRSPAICPA    50
    CNSTLSGKLD IVRTELSPSE EYKAMVLAGL RPEVVLDISS RALAFWTYQV 100
    HQERLYQEYN FSKAENHLKQ MEKMYMQQIQ SKNIELTSMK GEVISMKKVL 150
    EEYKKKFSDI SEKLMERNRQ YQKLQGLYDS LRLRNITIAS QEGSLEPGMI 200
    PQSGVFGFPP GNNSKFSLDH IPVGNQGGGD EDVQFRPFFV CSPTAPEPIN 250
    NFFSFASPSH EAEQQVCSRA FKAKRI 276
    Length:276
    Mass (Da):31,386
    Last modified:April 20, 2010 - v1
    Checksum:i8677CFEC187C1465
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC027184 Genomic DNA. No translation available.
    CH466605 Genomic DNA. Translation: EDL20823.1.
    CCDSiCCDS49477.1.
    RefSeqiNP_001104589.1. NM_001111119.1.
    XP_006519036.1. XM_006518973.1.
    UniGeneiMm.343880.

    Genome annotation databases

    EnsembliENSMUST00000095932; ENSMUSP00000093622; ENSMUSG00000071470.
    GeneIDi239083.
    KEGGimmu:239083.
    UCSCiuc011zjw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC027184 Genomic DNA. No translation available.
    CH466605 Genomic DNA. Translation: EDL20823.1 .
    CCDSi CCDS49477.1.
    RefSeqi NP_001104589.1. NM_001111119.1.
    XP_006519036.1. XM_006518973.1.
    UniGenei Mm.343880.

    3D structure databases

    ProteinModelPortali D3Z3K2.
    SMRi D3Z3K2. Positions 5-58.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000095932 ; ENSMUSP00000093622 ; ENSMUSG00000071470 .
    GeneIDi 239083.
    KEGGi mmu:239083.
    UCSCi uc011zjw.1. mouse.

    Organism-specific databases

    CTDi 57820.
    MGIi MGI:2685134. Ccnb1ip1.

    Phylogenomic databases

    GeneTreei ENSGT00390000002849.
    HOGENOMi HOG000111677.
    KOi K10639.
    OMAi IANHEGT.
    OrthoDBi EOG76MK99.
    PhylomeDBi D3Z3K2.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Miscellaneous databases

    NextBioi 383975.
    PROi D3Z3K2.
    SOURCEi Search...

    Family and domain databases

    InterProi IPR001841. Znf_RING.
    [Graphical view ]
    Pfami PF14634. zf-RING_5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. Cited for: FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF 77-LEU--VAL-110.
    4. "Evidence implicating CCNB1IP1, a RING domain-containing protein required for meiotic crossing over in mice, as an E3 SUMO ligase."
      Strong E.R., Schimenti J.C.
      Genes (Basel) 1:440-451(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    5. "Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination."
      Qiao H., Prasada Rao H.B., Yang Y., Fong J.H., Cloutier J.M., Deacon D.C., Nagel K.E., Swartz R.K., Strong E., Holloway J.K., Cohen P.E., Schimenti J., Ward J., Hunter N.
      Nat. Genet. 46:194-199(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiCIP1_MOUSE
    AccessioniPrimary (citable) accession number: D3Z3K2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 19, 2014
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3