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Protein

Scaffold attachment factor B1

Gene

Safb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.1 Publication

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • core promoter binding Source: UniProtKB
  • double-stranded DNA binding Source: GO_Central
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: MGI
  • sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • growth Source: MGI
  • hormone metabolic process Source: MGI
  • intracellular estrogen receptor signaling pathway Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • regulation of mRNA processing Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Scaffold attachment factor B1
Short name:
SAF-B1
Gene namesi
Name:Safb
Synonyms:Safb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2146974. Safb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004130222 – 937Scaffold attachment factor B1Add BLAST936

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei55PhosphoserineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Cross-linki172Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei188PhosphothreonineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei197PhosphoserineBy similarity1
Modified residuei209PhosphoserineBy similarity1
Cross-linki231Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki316Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei405PhosphoserineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Cross-linki505Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki565Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki600Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki600Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei602PhosphoserineBy similarity1
Modified residuei604PhosphoserineBy similarity1
Modified residuei623PhosphoserineBy similarity1
Modified residuei626PhosphoserineCombined sources1
Modified residuei629N6-acetyllysineBy similarity1
Modified residuei832Omega-N-methylarginineCombined sources1
Modified residuei890Asymmetric dimethylarginineCombined sources1
Modified residuei896Asymmetric dimethylarginineCombined sources1
Modified residuei906Asymmetric dimethylarginineCombined sources1
Modified residuei912Asymmetric dimethylarginineCombined sources1

Post-translational modificationi

Sumoylated by PIAS1 with SUMO1 and SUMO2/3, desumoylated by SENP1. Sumoylation is required for transcriptional repressor activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiD3YXK2.
MaxQBiD3YXK2.
PaxDbiD3YXK2.
PeptideAtlasiD3YXK2.
PRIDEiD3YXK2.

PTM databases

iPTMnetiD3YXK2.
PhosphoSitePlusiD3YXK2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000071054.
ExpressionAtlasiD3YXK2. baseline and differential.
GenevisibleiD3YXK2. MM.

Interactioni

Subunit structurei

Monomer. Can form homodimers. Interacts with KHDRBS3, POLR2A, SAFB2 or SFRS1, SFRS9 and TRA2B/SFRS10 (By similarity). Interacts with RBMX. Interacts with SRPK1 and inhibits its activity (By similarity).By similarity

Protein-protein interaction databases

BioGridi230338. 3 interactors.
IntActiD3YXK2. 1 interactor.
STRINGi10090.ENSMUSP00000092849.

Structurei

3D structure databases

ProteinModelPortaliD3YXK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 65SAPPROSITE-ProRule annotationAdd BLAST35
Domaini428 – 506RRMPROSITE-ProRule annotationAdd BLAST79

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni550 – 814Interaction with POLR2ABy similarityAdd BLAST265
Regioni621 – 937Interaction with SAFB2By similarityAdd BLAST317

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili652 – 726Sequence analysisAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi621 – 638Nuclear localization signalSequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi96 – 285Glu-richAdd BLAST190
Compositional biasi634 – 853Arg-richAdd BLAST220
Compositional biasi640 – 726Glu-richAdd BLAST87
Compositional biasi807 – 925Gly-richAdd BLAST119

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation
Contains 1 SAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4661. Eukaryota.
ENOG4111F1G. LUCA.
GeneTreeiENSGT00730000110777.
HOGENOMiHOG000092533.
InParanoidiD3YXK2.
PhylomeDBiD3YXK2.
TreeFamiTF325240.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D3YXK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETLSGLGD ASAAGAAAVS SAASETGTRR LSDLRVIDLR AELKKRNLDS
60 70 80 90 100
SGNKSVLMER LKKAIEDEGG NPDEIEVTSE CNKKMPKRPS KGRKPEDEGV
110 120 130 140 150
EDNGLEENSG DGQEDVETSL ENLQDMDMMD ISVLDEADID NGSVADCVEE
160 170 180 190 200
EEEATLPEGL ADSTELVEGD LKGLPEQLQE HAIDDKDTVN NVDTSSSDFT
210 220 230 240 250
MLQEMEEASL EPENEKILDI LGETCKSEPV KEEGSELEQP FAQATSSVGP
260 270 280 290 300
DRKLAEEEDL FESCGHPEEE EEEEEEDQEE EQEEEGDLAL ASSSKSESPS
310 320 330 340 350
TRCQWSEADA PLAVVKREPA DAPGGGTGMD REPVGLEEPV EQSSTAAQLP
360 370 380 390 400
EATSQELVRA PTAALSPEPQ DSKEDVKKFA FDACNDVPAP PKESSASEGA
410 420 430 440 450
DQKMSSVEED SDTKRLSREE KGRSSCGRNF WVSGLSSTTR ATDLKNLFSR
460 470 480 490 500
YGKVVGAKVV TNARSPGARC YGFVTMSTAE EATKCISHLH KTELHGKMIS
510 520 530 540 550
VEKAKSEPTG KRVPDRRDGD SKKEKASTSD RSANLKREEK GERKDDAKKT
560 570 580 590 600
DDGSTEKSKD ADDQKPGPSE RSRTTKSGSR GTERTVVMDK SKGVPVISVK
610 620 630 640 650
TSGSKERASK SQDRKSASRE KRSVVSFDKV KESRKSRDSE SRRERERSER
660 670 680 690 700
EQRLQAQWER EERERLEIAR ERLAFHRHRL ERERMERERL ERERMHVEQE
710 720 730 740 750
RRREQERIHR EREELRRQQE LRYEQERRPA VRRPYEVDGR RDDAYWPEAK
760 770 780 790 800
RAALDDRYHS DFSRQDRFHD FDHRDRGRYP NHSVDRREGS RSMMGDREGQ
810 820 830 840 850
HYPERHGGPE RHGRDSRDGW GYGSNKRLSE GRGLPPPPRR DWGEHGRRLE
860 870 880 890 900
DDRAWQGTAD GGMMERDHKR WQGGERSMSG HSGPGHMMNR GGMSGRGSFA
910 920 930
PGGASRGHVI PRGGMQAGFG GQSRGSRPSD ARFTRRY
Length:937
Mass (Da):105,104
Last modified:April 5, 2011 - v2
Checksum:iD246A419FA465C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT485788 Genomic DNA. No translation available.
CH466537 Genomic DNA. Translation: EDL38175.1.
BC018200 mRNA. No translation available.
AK087504 mRNA. No translation available.
CCDSiCCDS50152.1.
RefSeqiNP_001156772.1. NM_001163300.1.
UniGeneiMm.255066.

Genome annotation databases

EnsembliENSMUST00000095224; ENSMUSP00000092849; ENSMUSG00000071054.
GeneIDi224903.
KEGGimmu:224903.
UCSCiuc012avv.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT485788 Genomic DNA. No translation available.
CH466537 Genomic DNA. Translation: EDL38175.1.
BC018200 mRNA. No translation available.
AK087504 mRNA. No translation available.
CCDSiCCDS50152.1.
RefSeqiNP_001156772.1. NM_001163300.1.
UniGeneiMm.255066.

3D structure databases

ProteinModelPortaliD3YXK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230338. 3 interactors.
IntActiD3YXK2. 1 interactor.
STRINGi10090.ENSMUSP00000092849.

PTM databases

iPTMnetiD3YXK2.
PhosphoSitePlusiD3YXK2.

Proteomic databases

EPDiD3YXK2.
MaxQBiD3YXK2.
PaxDbiD3YXK2.
PeptideAtlasiD3YXK2.
PRIDEiD3YXK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095224; ENSMUSP00000092849; ENSMUSG00000071054.
GeneIDi224903.
KEGGimmu:224903.
UCSCiuc012avv.1. mouse.

Organism-specific databases

CTDi6294.
MGIiMGI:2146974. Safb.

Phylogenomic databases

eggNOGiKOG4661. Eukaryota.
ENOG4111F1G. LUCA.
GeneTreeiENSGT00730000110777.
HOGENOMiHOG000092533.
InParanoidiD3YXK2.
PhylomeDBiD3YXK2.
TreeFamiTF325240.

Miscellaneous databases

ChiTaRSiSafb. mouse.
PROiD3YXK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000071054.
ExpressionAtlasiD3YXK2. baseline and differential.
GenevisibleiD3YXK2. MM.

Family and domain databases

Gene3Di1.10.720.30. 1 hit.
3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003034. SAP_dom.
[Graphical view]
PfamiPF00076. RRM_1. 1 hit.
PF02037. SAP. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
SM00513. SAP. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50800. SAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSAFB1_MOUSE
AccessioniPrimary (citable) accession number: D3YXK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 5, 2011
Last modified: November 2, 2016
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.