ID   D3YWA9_MOUSE            Unreviewed;       453 AA.
AC   D3YWA9;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   27-MAR-2024, entry version 77.
DE   RecName: Full=Protein spinster homolog 1 {ECO:0000256|ARBA:ARBA00039482};
DE   AltName: Full=Spns1 {ECO:0000256|ARBA:ARBA00041462};
GN   Name=Spns1 {ECO:0000313|Ensembl:ENSMUSP00000112555.2,
GN   ECO:0000313|MGI:MGI:1920908};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090 {ECO:0000313|Ensembl:ENSMUSP00000112555.2, ECO:0000313|Proteomes:UP000000589};
RN   [1] {ECO:0000313|Ensembl:ENSMUSP00000112555.2, ECO:0000313|Proteomes:UP000000589}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000112555.2,
RC   ECO:0000313|Proteomes:UP000000589};
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2] {ECO:0000313|Ensembl:ENSMUSP00000112555.2}
RP   IDENTIFICATION.
RC   STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000112555.2};
RG   Ensembl;
RL   Submitted (NOV-2023) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H(+)(out)
CC         = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H(+)(in);
CC         Xref=Rhea:RHEA:74447, ChEBI:CHEBI:15378, ChEBI:CHEBI:77287;
CC         Evidence={ECO:0000256|ARBA:ARBA00036769};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) +
CC         H(+)(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) +
CC         H(+)(in); Xref=Rhea:RHEA:74455, ChEBI:CHEBI:15378, ChEBI:CHEBI:77288;
CC         Evidence={ECO:0000256|ARBA:ARBA00036994};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1-acyl-sn-glycero-3-phosphocholine(out) + H(+)(out) = a 1-
CC         acyl-sn-glycero-3-phosphocholine(in) + H(+)(in);
CC         Xref=Rhea:RHEA:74435, ChEBI:CHEBI:15378, ChEBI:CHEBI:58168;
CC         Evidence={ECO:0000256|ARBA:ARBA00035932};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H(+)(out) = a
CC         1-acyl-sn-glycero-3-phosphoethanolamine(in) + H(+)(in);
CC         Xref=Rhea:RHEA:74439, ChEBI:CHEBI:15378, ChEBI:CHEBI:64381;
CC         Evidence={ECO:0000256|ARBA:ARBA00036238};
CC   -!- SUBCELLULAR LOCATION: Lysosome membrane
CC       {ECO:0000256|ARBA:ARBA00004155}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004155}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Spinster
CC       (TC 2.A.1.49) family. {ECO:0000256|ARBA:ARBA00024338}.
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DR   AlphaFoldDB; D3YWA9; -.
DR   SMR; D3YWA9; -.
DR   EPD; D3YWA9; -.
DR   jPOST; D3YWA9; -.
DR   MaxQB; D3YWA9; -.
DR   PeptideAtlas; D3YWA9; -.
DR   ProteomicsDB; 303974; -.
DR   Antibodypedia; 26665; 85 antibodies from 20 providers.
DR   Ensembl; ENSMUST00000119754.8; ENSMUSP00000112555.2; ENSMUSG00000030741.16.
DR   AGR; MGI:1920908; -.
DR   MGI; MGI:1920908; Spns1.
DR   VEuPathDB; HostDB:ENSMUSG00000030741; -.
DR   GeneTree; ENSGT00390000005976; -.
DR   HOGENOM; CLU_001265_5_12_1; -.
DR   ChiTaRS; Spns1; mouse.
DR   Proteomes; UP000000589; Chromosome 7.
DR   Bgee; ENSMUSG00000030741; Expressed in yolk sac and 228 other cell types or tissues.
DR   ExpressionAtlas; D3YWA9; baseline and differential.
DR   GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   CDD; cd17328; MFS_spinster_like; 1.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR044770; MFS_spinster-like.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR23505:SF13; PROTEIN SPINSTER HOMOLOG 1; 1.
DR   PANTHER; PTHR23505; SPINSTER; 1.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|EPD:D3YWA9,
KW   ECO:0007829|MaxQB:D3YWA9};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000589};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT   TRANSMEM        57..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        99..119
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        126..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        165..181
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        188..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        273..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        323..344
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        356..375
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   TRANSMEM        381..411
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          61..453
FT                   /note="Major facilitator superfamily (MFS) profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50850"
FT   REGION          1..38
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   453 AA;  48281 MW;  2918BACA2FF38834 CRC64;
     MAGSDTAPFL SQADDPDDGP APGHPGLPGP MGNPKSGELE VPDCEGLQRI TGLSRGHSTL
     IVVVLCYINL LNYMDRFTVA GVLTDIEQFF NIGDGSTGLI QTVFISSYMV LAPVFGYLGD
     RYNRKYLMCG GIAFWSLVTL GSSFIPREHF WLLLLTRGLV GVGEASYSTI APTLIADLFV
     ADQRSRMLSI FYFAIPVGSG LGYIAGSKVK DVAGDWHWAL RVTPGLGVLA VLLLFLVVQE
     PPRGAVERHS GSPPLSPTSW WADLKALARN PSFVLSSLGF TSVAFVTGSL ALWAPAFLLR
     SRVVLGETPP CLPGDSCSSS DSLIFGLITC LTGVLGVGLG VEISRRLRRF NPRADPLVCA
     AGLLGSAPFL FLALACARGS IVATYIFIFI GETLLSMNWA IVADILLYVV IPTRRSTAEA
     FQIVLSHLLG DAGSPYLIGL VCCMSLGPQM TGL
//