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Protein

A-kinase anchor protein 5

Gene

Akap5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May anchor the PKA kinase to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors.By similarity

GO - Molecular functioni

  • adenylate cyclase binding Source: MGI
  • protein kinase A regulatory subunit binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-399719. Trafficking of AMPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 5
Short name:
AKAP-5
Alternative name(s):
A-kinase anchor protein 150 kDa
Short name:
AKAP 150
Short name:
P150
cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein
Gene namesi
Name:Akap5
Synonyms:Akap150
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2685104. Akap5.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 745745A-kinase anchor protein 5PRO_0000414750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineCombined sources
Modified residuei22 – 221PhosphoserineCombined sources
Lipidationi36 – 361S-palmitoyl cysteineBy similarity
Lipidationi123 – 1231S-palmitoyl cysteineBy similarity

Post-translational modificationi

Palmitoylation at Cys-36 and Cys-123 plays a key role in targeting to lipid rafts.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiD3YVF0.
PaxDbiD3YVF0.
PeptideAtlasiD3YVF0.
PRIDEiD3YVF0.

PTM databases

iPTMnetiD3YVF0.
PhosphoSiteiD3YVF0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000021057.
ExpressionAtlasiD3YVF0. baseline and differential.
GenevisibleiD3YVF0. MM.

Interactioni

Subunit structurei

Interacts with ADCY8, and enhances its phosphorylation at lipid rafts. Binds dimer of the RII-beta regulatory subunit of cAMP-dependent protein kinase (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnk2P974383EBI-7091108,EBI-7091062
Ppp3cbP484533EBI-7091108,EBI-642618
Prkar2aP123672EBI-7091108,EBI-645747

GO - Molecular functioni

  • adenylate cyclase binding Source: MGI
  • protein kinase A regulatory subunit binding Source: MGI

Protein-protein interaction databases

BioGridi231964. 3 interactions.
IntActiD3YVF0. 4 interactions.
MINTiMINT-8412175.
STRINGi10090.ENSMUSP00000114495.

Structurei

3D structure databases

ProteinModelPortaliD3YVF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 10030AKAPAdd
BLAST
Repeati304 – 31291; approximate
Repeati320 – 32782; approximate
Repeati328 – 33583; approximate
Repeati348 – 35584; approximate
Repeati356 – 36385; approximate
Repeati364 – 395326; approximateAdd
BLAST
Repeati420 – 436177; approximateAdd
BLAST
Repeati445 – 45288; approximate
Repeati461 – 46889
Repeati469 – 476810
Repeati477 – 484811
Repeati485 – 492812
Repeati493 – 500813
Repeati501 – 508814; approximate
Repeati517 – 524815; approximate
Repeati525 – 532816
Repeati533 – 540817
Repeati541 – 548818
Repeati549 – 556819
Repeati557 – 564820; approximate
Repeati565 – 572821
Repeati573 – 580822; approximate
Repeati581 – 588823; approximate
Repeati589 – 596824; approximate
Repeati597 – 604825
Repeati605 – 612826; approximate
Repeati613 – 620827; approximate
Repeati621 – 628828; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 164164Essential to the intracellular anchoring functionBy similarityAdd
BLAST
Regioni304 – 62832528 X 8 AA repeats of V-G-Q-A-E-E-A-TAdd
BLAST
Regioni706 – 72722RII-beta subunit binding domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi332 – 654323Glu-richAdd
BLAST

Domaini

The N-terminal region, which is highly basic, is required for interaction with calmodulin.By similarity

Sequence similaritiesi

Contains 1 AKAP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IIWH. Eukaryota.
ENOG4111833. LUCA.
GeneTreeiENSGT00390000019941.
HOGENOMiHOG000168350.
InParanoidiD3YVF0.
KOiK16522.
PhylomeDBiD3YVF0.

Family and domain databases

InterProiIPR001573. Pkinase-A_anch_WSK-motif.
[Graphical view]
PfamiPF03832. WSK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3YVF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METSVSEIQV ETKDEKGPVA ASPQKERQER KTATLCFKRR KKANKTKPKA
60 70 80 90 100
GSRTAEETKK HTPEAGGSGQ RQPAGAWASI KGLVTHRKRS EPAKKQKPPE
110 120 130 140 150
AEVQPEDGAL PKKKAKSRLK FPCLRFSRGA KRSRHSKLTE DSGYVRVQGE
160 170 180 190 200
ADDLEIKAQT QPDDQAIQAG STQGLQEGVL VRDGKKSQES HISNSVTSGE
210 220 230 240 250
NVIAIELELE NKSSAIQMGT PELEKETKVI TEKPSVQTQR ASLLESSAAG
260 270 280 290 300
SPRSVTSAAP PSPATTHQHS LEEPSNGIRE SAPSGKDDRR KTAAEEKKSG
310 320 330 340 350
ETALGQAEEA AVGQADKRAL SQAGEATAGH PEEATVIQAE SQAKEGKLSQ
360 370 380 390 400
AEETTVAQAK ETVLSQAKEG ELSQAKKATV GQAEEATIDH TEKVTVDQAE
410 420 430 440 450
ETTVGQAEEA TVGQAGEAIL SQAKEATVVG QAEEATVDRA EEATVGQAEE
460 470 480 490 500
ATVGHTEKVT VDQAEEATVG QAEEATVGQA EEATVDWAEK PTVGQAEEAT
510 520 530 540 550
VGQAEEATVG HTEKVTVDQA EEATVGQAEE ATVGHTEKVT VDHAEEATVG
560 570 580 590 600
QAEEATVGQA EKVTVDHAEE ATVGQAEEAT VGQAEKVTVD HAEEATVGQA
610 620 630 640 650
EEATVGQAEK VTVDQAEEPT VDQAEEAISS HAPDLKENGI DTEKPRSEES
660 670 680 690 700
KRMEPIAIII TDTEISEFDV KKSKNVPKQF LISMENEQVG VFANDSDFEG
710 720 730 740
RTSEQYETLL IETASSLVKN AIELSVEQLV NEMVSEDNQI NTLFQ
Length:745
Mass (Da):79,397
Last modified:April 5, 2011 - v2
Checksum:iB0A2949CC0F52569
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC120002 Genomic DNA. No translation available.
CCDSiCCDS49092.1.
RefSeqiNP_001094941.1. NM_001101471.1.
UniGeneiMm.311452.

Genome annotation databases

EnsembliENSMUST00000154078; ENSMUSP00000114495; ENSMUSG00000021057.
GeneIDi238276.
KEGGimmu:238276.
UCSCiuc007nya.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC120002 Genomic DNA. No translation available.
CCDSiCCDS49092.1.
RefSeqiNP_001094941.1. NM_001101471.1.
UniGeneiMm.311452.

3D structure databases

ProteinModelPortaliD3YVF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231964. 3 interactions.
IntActiD3YVF0. 4 interactions.
MINTiMINT-8412175.
STRINGi10090.ENSMUSP00000114495.

PTM databases

iPTMnetiD3YVF0.
PhosphoSiteiD3YVF0.

Proteomic databases

MaxQBiD3YVF0.
PaxDbiD3YVF0.
PeptideAtlasiD3YVF0.
PRIDEiD3YVF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000154078; ENSMUSP00000114495; ENSMUSG00000021057.
GeneIDi238276.
KEGGimmu:238276.
UCSCiuc007nya.2. mouse.

Organism-specific databases

CTDi9495.
MGIiMGI:2685104. Akap5.

Phylogenomic databases

eggNOGiENOG410IIWH. Eukaryota.
ENOG4111833. LUCA.
GeneTreeiENSGT00390000019941.
HOGENOMiHOG000168350.
InParanoidiD3YVF0.
KOiK16522.
PhylomeDBiD3YVF0.

Enzyme and pathway databases

ReactomeiR-MMU-399719. Trafficking of AMPA receptors.

Miscellaneous databases

PROiD3YVF0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021057.
ExpressionAtlasiD3YVF0. baseline and differential.
GenevisibleiD3YVF0. MM.

Family and domain databases

InterProiIPR001573. Pkinase-A_anch_WSK-motif.
[Graphical view]
PfamiPF03832. WSK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP5_MOUSE
AccessioniPrimary (citable) accession number: D3YVF0
Secondary accession number(s): D3Z5C9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: April 5, 2011
Last modified: September 7, 2016
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.