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D3V8R3 (D3V8R3_XENNA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity. RuleBase RU004445 HAMAP-Rule MF_01033 SAAS SAAS001804

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. RuleBase RU004445 HAMAP-Rule MF_01033 SAAS SAAS001804

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. RuleBase RU004445 HAMAP-Rule MF_01033 SAAS SAAS001804

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. RuleBase RU004445 HAMAP-Rule MF_01033 SAAS SAAS001804

Subunit structure

Homodimer By similarity. RuleBase RU004445 HAMAP-Rule MF_01033 SAAS SAAS001804

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. HAMAP-Rule MF_01033

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding94 – 10714NAD By similarity HAMAP-Rule MF_01033
Nucleotide binding301 – 31313NAD By similarity HAMAP-Rule MF_01033

Sites

Metal binding2431Magnesium or manganese By similarity HAMAP-Rule MF_01033
Metal binding2671Magnesium or manganese By similarity HAMAP-Rule MF_01033
Metal binding2711Magnesium or manganese By similarity HAMAP-Rule MF_01033
Binding site1151Substrate By similarity HAMAP-Rule MF_01033
Binding site1251Substrate By similarity HAMAP-Rule MF_01033
Binding site1541Substrate By similarity HAMAP-Rule MF_01033
Binding site2431Substrate By similarity HAMAP-Rule MF_01033
Site1611Important for catalysis By similarity HAMAP-Rule MF_01033
Site2111Important for catalysis By similarity HAMAP-Rule MF_01033

Sequences

Sequence LengthMass (Da)Tools
D3V8R3 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 19BE10E3F3374F27

FASTA38943,187
        10         20         30         40         50         60 
MSSSQSISNN TIANKQKANH FHIAVLPGDG IGPEVMKQAY KILDAIRHRF NIRITTSEYD 

        70         80         90        100        110        120 
IGGIAIDRHG SPLPQETVIG CEKADAILFG SVGGPKWEHL PPSQQPERGA LLPLRKHFKL 

       130        140        150        160        170        180 
FSNLRPARLY RGLESFCPLR HDIATKGFDI LCVRELTGGI YFGQPKGREG KGKYERAFDT 

       190        200        210        220        230        240 
EIYHRFEIER IAHIAFQSAR KRRHKVTSID KANVLQSSVL WREIVSEIAK DYPDVEINHM 

       250        260        270        280        290        300 
YIDNATMQLI KNPAQFDVLL CSNIFGDILS DECAMITGSM GMLPSASLNE KGFGLYEPAG 

       310        320        330        340        350        360 
GSAPDIAGKN IANPIAQILS VALLLRYSFE CNDAANAIEQ AINQALEQGY RTADLTSNDN 

       370        380 
TISTDEMGDI IARYITEEYR KTISLSSLT

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References

[1]"Complete genome sequence of Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6)."
Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S., Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P., Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S., Suen G.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		FN667742 Genomic DNA. Translation: CBJ89111.1.
RefSeqYP_003711322.1. NC_014228.1.

3D structure databases

ProteinModelPortalD3V8R3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCBJ89111; CBJ89111; XNC1_1040.
GeneID9433081.
KEGGxne:XNC1_1040.
PATRIC42461937. VBIXenNem38452_0958.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000021112.
KOK00052.

Enzyme and pathway databases

UniPathwayUPA00048; UER00072.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_01033. LeuB_type1.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
PANTHERPTHR11835. PTHR11835. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. leuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD3V8R3_XENNA
AccessionPrimary (citable) accession number: D3V8R3
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 1, 2013
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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