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D3V6X6

- D3V6X6_XENBS

UniProt

D3V6X6 - D3V6X6_XENBS

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Xenorhabdus bovienii (strain SS-2004)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
    Metal bindingi107 – 1071Magnesium; catalyticUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei217 – 2171Allosteric activator ADPUniRule annotation
    Binding sitei228 – 2281SubstrateUniRule annotation
    Binding sitei249 – 2491Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772ATPUniRule annotation
    Nucleotide bindingi106 – 1094ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciXBOV406818:GHLH-4305-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotationImported
    Ordered Locus Names:XBJ1_4302Imported
    OrganismiXenorhabdus bovienii (strain SS-2004)Imported
    Taxonomic identifieri406818 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeXenorhabdus
    ProteomesiUP000002045: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD3V6X6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 295Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
    Regioni219 – 2213Allosteric activator ADP bindingUniRule annotation
    Regioni255 – 2584Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3V6X6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINKIKRIGV LTSGGDAPGM NAAIRGVVRV ALTEGLEVYG IYDGYLGLYE    50
    NRMKKLDRFS VSDMINRGGT FLGSARFPEF RDDKVREIAI ENMRKNDIDA 100
    LVVIGGDGSY LGAKKLTEAG FPCIGLPGTI DNDVAGTDYT IGYFTALETV 150
    VEAIDRLRDT STSHKRISIV EVMGRYCGDL TLSAAIAGGC EFIVLPENEI 200
    PFDREELLAE IKAGIQKGKR HAIVAITEHV CNVDELAKYI EAETRHETRA 250
    TVLGHIQRGG APVAYDRILA SRMGAYSVQL LMEGFGGRCV GIQNEKLVHH 300
    DIIDAVQNMQ RVFKKDWLET AKQLY 325
    Length:325
    Mass (Da):35,775
    Last modified:April 20, 2010 - v1
    Checksum:iE3A82561775ADB5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN667741 Genomic DNA. Translation: CBJ83405.1.
    RefSeqiWP_012990544.1. NC_013892.1.
    YP_003470163.1. NC_013892.1.

    Genome annotation databases

    EnsemblBacteriaiCBJ83405; CBJ83405; XBJ1_4302.
    GeneIDi8833935.
    KEGGixbo:XBJ1_4302.
    PATRICi35303161. VBIXenBov95754_3872.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN667741 Genomic DNA. Translation: CBJ83405.1 .
    RefSeqi WP_012990544.1. NC_013892.1.
    YP_003470163.1. NC_013892.1.

    3D structure databases

    ProteinModelPortali D3V6X6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBJ83405 ; CBJ83405 ; XBJ1_4302 .
    GeneIDi 8833935.
    KEGGi xbo:XBJ1_4302.
    PATRICi 35303161. VBIXenBov95754_3872.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci XBOV406818:GHLH-4305-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6)."
      Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S., Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P., Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S., Suen G.
      Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS-2004Imported.
    2. "The entomopathogenic bacterial endosymbionts xenorhabdus and photorhabdus: convergent lifestyles from divergent genomes."
      Chaston J.M., Suen G., Tucker S.L., Andersen A.W., Bhasin A., Bode E., Bode H.B., Brachmann A.O., Cowles C.E., Cowles K.N., Darby C., de Leon L., Drace K., Du Z., Givaudan A., Herbert Tran E.E., Jewell K.A., Knack J.J.
      , Krasomil-Osterfeld K.C., Kukor R., Lanois A., Latreille P., Leimgruber N.K., Lipke C.M., Liu R., Lu X., Martens E.C., Marri P.R., Medigue C., Menard M.L., Miller N.M., Morales-Soto N., Norton S., Ogier J.C., Orchard S.S., Park D., Park Y., Qurollo B.A., Sugar D.R., Richards G.R., Rouy Z., Slominski B., Slominski K., Snyder H., Tjaden B.C., van der Hoeven R., Welch R.D., Wheeler C., Xiang B., Barbazuk B., Gaudriault S., Goodner B., Slater S.C., Forst S., Goldman B.S., Goodrich-Blair H.
      PLoS ONE 6:e27909-e27909(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SS-2004Imported.

    Entry informationi

    Entry nameiD3V6X6_XENBS
    AccessioniPrimary (citable) accession number: D3V6X6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

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