Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA topoisomerase 1

Gene

topA

Organism
Xenorhabdus bovienii (strain SS-2004)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+SAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei317O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, Isomerase, TopoisomeraseUniRule annotationSAAS annotation
LigandMagnesiumSAAS annotation, Metal-bindingSAAS annotation

Enzyme and pathway databases

BioCyciXBOV406818:G1GIZ-1985-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Gene namesi
Name:topAUniRule annotationImported
Ordered Locus Names:XBJ1_2289Imported
OrganismiXenorhabdus bovienii (strain SS-2004)Imported
Taxonomic identifieri406818 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeXenorhabdus
Proteomesi
  • UP000002045 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei33Interaction with DNAUniRule annotation1
Sitei166Interaction with DNAUniRule annotation1
Sitei167Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei175Interaction with DNAUniRule annotation1
Sitei182Interaction with DNAUniRule annotation1
Sitei319Interaction with DNAUniRule annotation1
Sitei505Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi406818.XBJ1_2289

Structurei

3D structure databases

ProteinModelPortaliD3V265
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 140ToprimInterPro annotationAdd BLAST138

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 195Interaction with DNAUniRule annotation6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili371 – 391Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105C73 Bacteria
COG0550 LUCA
COG0551 LUCA
HOGENOMiHOG000004018
KOiK03168
OMAiPECKYTR
OrthoDBiPOG091H02BN

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR013263 TopoI_Znr_bac
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PANTHERiPTHR42785 PTHR42785, 1 hit
PfamiView protein in Pfam
PF08272 Topo_Zn_Ribbon, 2 hits
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

D3V265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKALVIVES PAKAKTINKY LGNDYVVKSS VGHIRDLPTS GAASQKSSNS
60 70 80 90 100
STDKNKKKKD EKTALVNRMG VDPYHGWEAD YQILPGKEKV VAELKMLAEK
110 120 130 140 150
ADHIYLATDL DREGEAIAWH LREVIGGGEA RFSRVVFNEI TKNAITQAFD
160 170 180 190 200
NPGELNIDRV NAQQARRFMD RVVGYMVSPL LWKKVARGLS AGRVQSVAVR
210 220 230 240 250
LIVEREREIK AFVPEEYWQL HADLMAKGDI DLHMEVTHEG GKPFKPINAQ
260 270 280 290 300
QTQAAVSLLE KARYKVLDRE DRPTSSKPGA PFITSTLQQA ASTRLGFGVK
310 320 330 340 350
KTMMMAQRLY EAGHITYMRT DSTNLSQDAL DMVRGYINDQ FGDKYLPKTA
360 370 380 390 400
NIYSNKENSQ EAHEAIRPSS VDVMAEELKD MEADAQKLYQ LIWRQFIACQ
410 420 430 440 450
MMPAKYDSTT LTIQAGNFEL RAKGRTLRFD GWTKVMPALR KGDEDHTLPV
460 470 480 490 500
VEVGTELDLK QLSPSQHFTK PSARFSEASL VRELEKRGIG RPSTYASIIS
510 520 530 540 550
TIQDRGYVRG ENRRFYAEKM GEIVNDRLEE NFNELMSYDF TARMEDQLDH
560 570 580 590 600
VANNEANWKG VLDEFFTNFS EQLDVASKDP DEGGMRPNPM VSTSIDCPTC
610 620 630 640 650
SRPMGIRTAT TGVFLGCSGY TLSPKERCKQ TINLIPENEI LNILEGDEAE
660 670 680 690 700
TNALRARHRC KECGTAMDSY LIDNQRKLHV CGNNPACDGY EVEEGEFRIK
710 720 730 740 750
GYDGPIVECD KCGSEMHLKM GRFGKYMGCT SEECKNTRKI LRSGEVAPPK
760 770 780 790 800
EDPVPLPELP CEKSDAYFVL RDGAAGVFLA ASTFPKSRET RAPLVEELVK
810 820 830 840 850
FKDRLPEKLC YLAKAPVADP EGHKTVVRFS RKTKQQYVSS EKNGKATGWA
860
AFFVDGAWVV KEK
Length:863
Mass (Da):96,862
Last modified:April 20, 2010 - v1
Checksum:iBEBAEDE670B084B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN667741 Genomic DNA Translation: CBJ81415.1
RefSeqiWP_012988731.1, NC_013892.1

Genome annotation databases

EnsemblBacteriaiCBJ81415; CBJ81415; XBJ1_2289
GeneIDi8831902
KEGGixbo:XBJ1_2289
PATRICifig|406818.4.peg.2063

Similar proteinsi

Entry informationi

Entry nameiD3V265_XENBS
AccessioniPrimary (citable) accession number: D3V265
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: February 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health