D3UWV6 (APHA_XENBS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Class B acid phosphatase
Short name=CBAP
EC=3.1.3.2

Gene names

Name:	aphA
Ordered Locus Names:	XBJ1_0800

Organism

Xenorhabdus bovienii (strain SS-2004) [Complete proteome] [HAMAP]

Taxonomic identifier

406818 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Xenorhabdus › Xenorhabdus bovienii

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dephosphorylates several organic phosphate monoesters. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds By similarity.
Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the class B bacterial acid phosphatase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular_function	acid phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 237	214	Class B acid phosphatase		PRO_0000415230
Regions
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	69	1	Nucleophile By similarity
	Active site	71	1	Proton donor By similarity
	Metal binding	69	1	Magnesium By similarity
	Metal binding	71	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	192	1	Magnesium By similarity
	Binding site	177	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	D3UWV6 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: 5BA5B902EC8351AF	FASTA	237	26,870
	10 20 30 40 50 60 MRKVTLVFSA IAFAFSLNGV VQAKVQMPES VSSGVTTVEL SQRQAVHWVS VEQIEKSLQD 70 80 90 100 110 120 QPPMAIGFDI DDTVLFSSPG FYRGKLKYSP NDNSYLKNPA FWEKMNNEWD EFSMPKQIGI 130 140 150 160 170 180 ELVQMHLKRG DNIYFITGRT KTKTETVTKY LQEDLHIPAD KMNVVIFAGD DPGKNNKISW 190 200 210 220 230 MKEHKLKLYY GDADADIAAA HELNIRGIRI LRASNSSYQP LPKAGRFGEE VVIKSEY

References

[1]

"Complete genome sequence of Xenorhabdus nematophila (strain ATCC 19061 / DSM 3370 / LMG 1036 / NCIB 9965 / AN6)." Goodrich-Blair H., Barbazuk B., Bode H.B., Darby C., Du Z., Forst S., Gaudriault S., Goldman B.S., Goodner B., Henkhaus J., Latreille P., Medigue C., Miller N., Norton S., Ogier J.C., Rouy Z., Slater S., Suen G. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SS-2004.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FN667741 Genomic DNA. Translation: CBJ79941.1.
RefSeq	YP_003466735.1. NC_013892.1.
3D structure databases
ProteinModelPortal	D3UWV6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CBJ79941; CBJ79941; XBJ1_0800.
GeneID	8830427.
KEGG	xbo:XBJ1_0800.
PATRIC	35296696. VBIXenBov95754_0721.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000270623.
KO	K03788.
Enzyme and pathway databases
BioCyc	XBOV406818:GHLH-800-MONOMER.
Family and domain databases
Gene3D	3.40.50.1000. 1 hit.
InterPro	IPR005519. Acid_phosphat_B. IPR023214. HAD-like_dom. IPR010025. HAD-SF_ppase_IIIB_AphA. [Graphical view]
Pfam	PF03767. Acid_phosphat_B. 1 hit. [Graphical view]
PIRSF	PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01672. AphA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APHA_XENBS

Accession

Primary (citable) accession number: D3UWV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	April 20, 2010
Last modified:	May 29, 2013
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program