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D3UNH6

- D3UNH6_LISSS

UniProt

D3UNH6 - D3UNH6_LISSS

Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

    Catalytic activityi

    L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotation
    [IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei265 – 2651ATPUniRule annotation
    Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
    Binding sitei296 – 2961ATPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi183 – 1842ATPUniRule annotation
    Nucleotide bindingi208 – 2092ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. sulfurtransferase activity Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. thiamine biosynthetic process Source: UniProtKB-HAMAP
    2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
    3. tRNA thio-modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    TransferaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    Thiamine biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciLSEE683837:GI10-1531-MONOMER.
    UniPathwayiUPA00060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable tRNA sulfurtransferaseUniRule annotation (EC:2.8.1.4UniRule annotation)
    Alternative name(s):
    Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
    Thiamine biosynthesis protein ThiIUniRule annotation
    tRNA 4-thiouridine synthaseUniRule annotation
    Gene namesi
    Name:thiIUniRule annotationImported
    Ordered Locus Names:lse_1508Imported
    OrganismiListeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)Imported
    Taxonomic identifieri683837 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
    ProteomesiUP000002044: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini60 – 165106THUMPUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ThiI family.UniRule annotation
    Contains 1 THUMP domain.UniRule annotation
    Contains THUMP domain.SAAS annotation

    Phylogenomic databases

    HOGENOMiHOG000227470.
    KOiK03151.
    OMAiAQMKGPD.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    HAMAPiMF_00021. ThiI.
    InterProiIPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view]
    PfamiPF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view]
    SMARTiSM00981. THUMP. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
    PROSITEiPS51165. THUMP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3UNH6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFDRMLIRY GELSTKGKNR KQFVTKLAQN VKRAMQDLPE VRIHGERDRM    50
    YIILNGADYH LAEERLKPIF GIQSFSPAVR VDLDLDEVKN AALALVQDAH 100
    EENGTFKVAA RRSHREFPLD SNEINQEIGA HVLQNIADLT VNVKNPDVKL 150
    TIDVRKEGVF LSCRTILGAA GLPVGSSGRA MLMLSGGIDS PVAGYLAQKR 200
    GVEIEAVHFH SPPYTSEQAK QKAIDLAAKL AKYSGQVQMH IVPFTEIQEV 250
    IKQQIPESVI MTVTRRMMLR ITDELRRKRN GLAIVNGESL GQVASQTLES 300
    MLAINAVTAT PIIRPVVSMD KNEIITIAQK IDTYNLSVQP FEDCCTIFTP 350
    PSPKTKPKLD KIEHYESFTD FEALIKKAID NIETISVNIA ETEQVKDEFA 400
    DLF 403
    Length:403
    Mass (Da):45,068
    Last modified:April 20, 2010 - v1
    Checksum:iD1D9BCF7E2F1738F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN557490 Genomic DNA. Translation: CBH27659.1.
    RefSeqiWP_012985733.1. NC_013891.1.
    YP_003464745.1. NC_013891.1.

    Genome annotation databases

    EnsemblBacteriaiCBH27659; CBH27659; lse_1508.
    GeneIDi9082934.
    KEGGilsg:lse_1508.
    PATRICi32262913. VBILisSee138575_1503.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FN557490 Genomic DNA. Translation: CBH27659.1 .
    RefSeqi WP_012985733.1. NC_013891.1.
    YP_003464745.1. NC_013891.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CBH27659 ; CBH27659 ; lse_1508 .
    GeneIDi 9082934.
    KEGGi lsg:lse_1508.
    PATRICi 32262913. VBILisSee138575_1503.

    Phylogenomic databases

    HOGENOMi HOG000227470.
    KOi K03151.
    OMAi AQMKGPD.

    Enzyme and pathway databases

    UniPathwayi UPA00060 .
    BioCyci LSEE683837:GI10-1531-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    HAMAPi MF_00021. ThiI.
    InterProi IPR014729. Rossmann-like_a/b/a_fold.
    IPR020536. ThiI_AANH.
    IPR004114. THUMP.
    IPR003720. tRNA_STrfase.
    [Graphical view ]
    Pfami PF02568. ThiI. 1 hit.
    PF02926. THUMP. 1 hit.
    [Graphical view ]
    SMARTi SM00981. THUMP. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00342. TIGR00342. 1 hit.
    PROSITEi PS51165. THUMP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954Imported.

    Entry informationi

    Entry nameiD3UNH6_LISSS
    AccessioniPrimary (citable) accession number: D3UNH6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3