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Protein

Probable tRNA sulfurtransferase

Gene

thiI

Organism
Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.UniRule annotationSAAS annotation

Catalytic activityi

L-cysteine + 'activated' tRNA = L-serine + tRNA containing a thionucleotide.UniRule annotationSAAS annotation
[IscS]-SSH + [ThiS]-COAMP = [IscS]-SH + [ThiS]-COSH + AMP.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei265 – 2651ATPUniRule annotation
Binding sitei287 – 2871ATP; via amide nitrogenUniRule annotation
Binding sitei296 – 2961ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi183 – 1842ATPUniRule annotation
Nucleotide bindingi208 – 2092ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. sulfurtransferase activity Source: UniProtKB-HAMAP
  3. tRNA adenylyltransferase activity Source: InterPro
  4. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-HAMAP
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
  3. tRNA thio-modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Thiamine biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding, RNA-binding, tRNA-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciLSEE683837:GI10-1531-MONOMER.
UniPathwayiUPA00060.
UPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA sulfurtransferaseUniRule annotationSAAS annotation (EC:2.8.1.4UniRule annotationSAAS annotation)
Alternative name(s):
Sulfur carrier protein ThiS sulfurtransferaseUniRule annotation
Thiamine biosynthesis protein ThiIUniRule annotation
tRNA 4-thiouridine synthaseUniRule annotation
Gene namesi
Name:thiIUniRule annotationImported
Ordered Locus Names:lse_1508Imported
OrganismiListeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)Imported
Taxonomic identifieri683837 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002044: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 165106THUMPUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ThiI family.UniRule annotation
Contains 1 THUMP domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000227470.
KOiK03151.
OMAiAQKIDTF.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3UNH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFDRMLIRY GELSTKGKNR KQFVTKLAQN VKRAMQDLPE VRIHGERDRM
60 70 80 90 100
YIILNGADYH LAEERLKPIF GIQSFSPAVR VDLDLDEVKN AALALVQDAH
110 120 130 140 150
EENGTFKVAA RRSHREFPLD SNEINQEIGA HVLQNIADLT VNVKNPDVKL
160 170 180 190 200
TIDVRKEGVF LSCRTILGAA GLPVGSSGRA MLMLSGGIDS PVAGYLAQKR
210 220 230 240 250
GVEIEAVHFH SPPYTSEQAK QKAIDLAAKL AKYSGQVQMH IVPFTEIQEV
260 270 280 290 300
IKQQIPESVI MTVTRRMMLR ITDELRRKRN GLAIVNGESL GQVASQTLES
310 320 330 340 350
MLAINAVTAT PIIRPVVSMD KNEIITIAQK IDTYNLSVQP FEDCCTIFTP
360 370 380 390 400
PSPKTKPKLD KIEHYESFTD FEALIKKAID NIETISVNIA ETEQVKDEFA

DLF
Length:403
Mass (Da):45,068
Last modified:April 20, 2010 - v1
Checksum:iD1D9BCF7E2F1738F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN557490 Genomic DNA. Translation: CBH27659.1.
RefSeqiWP_012985733.1. NC_013891.1.
YP_003464745.1. NC_013891.1.

Genome annotation databases

EnsemblBacteriaiCBH27659; CBH27659; lse_1508.
GeneIDi9082934.
KEGGilsg:lse_1508.
PATRICi32262913. VBILisSee138575_1503.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN557490 Genomic DNA. Translation: CBH27659.1.
RefSeqiWP_012985733.1. NC_013891.1.
YP_003464745.1. NC_013891.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBH27659; CBH27659; lse_1508.
GeneIDi9082934.
KEGGilsg:lse_1508.
PATRICi32262913. VBILisSee138575_1503.

Phylogenomic databases

HOGENOMiHOG000227470.
KOiK03151.
OMAiAQKIDTF.

Enzyme and pathway databases

UniPathwayiUPA00060.
UPA00060.
BioCyciLSEE683837:GI10-1531-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00021. ThiI.
InterProiIPR014729. Rossmann-like_a/b/a_fold.
IPR020536. ThiI_AANH.
IPR004114. THUMP.
IPR003720. tRNA_STrfase.
[Graphical view]
PfamiPF02568. ThiI. 1 hit.
PF02926. THUMP. 1 hit.
[Graphical view]
SMARTiSM00981. THUMP. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00342. TIGR00342. 1 hit.
PROSITEiPS51165. THUMP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954Imported.

Entry informationi

Entry nameiD3UNH6_LISSS
AccessioniPrimary (citable) accession number: D3UNH6
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: March 4, 2015
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.