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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

gid

Organism
Listeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Enzyme and pathway databases

BioCyciLSEE683837:GI10-1218-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:gidImported
Synonyms:trmFOUniRule annotation
Ordered Locus Names:lse_1195Imported
OrganismiListeria seeligeri serovar 1/2b (strain ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954)Imported
Taxonomic identifieri683837 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
ProteomesiUP000002044 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD3UML3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3UML3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKRVNVIGA GLAGSEATWQ LVKRGIEVDL YEMRPVKQTP AHHTDKFAEL
60 70 80 90 100
VCTNSLRANG LTNAVGVIKE EMRILDSIII EAADEASVPA GGALAVDRHE
110 120 130 140 150
FSGYITDKVK NHPLVTVHTE EVTTIPTGPT IIATGPLTSP ALAEEIKRLT
160 170 180 190 200
GEEYLYFYDA AAPIIEKESI DMNKVYLKSR YDKGEAAYLN CPMSEEEFKA
210 220 230 240 250
FYEALVTAET AALKDFEKEV FFEGCMPIEV MAKRGIKTML FGPLKPVGLE
260 270 280 290 300
DPKNGKRPYA VLQLRQDDAA GSLYNMVGFQ THLKWGEQKR VFGMIPGLEN
310 320 330 340 350
AEIVRYGVMH RNTFINSPTV LEPTYQLKSR NDLFFAGQMT GVEGYVESAA
360 370 380 390 400
SGLAAGINAA NFVEEKELII FPKETAIGSL AHYITSASKK SFQPMNVNFG
410 420 430
LFPELASKIR VKQERNEKLA ERALNAIKGV AEGL
Length:434
Mass (Da):47,849
Last modified:April 20, 2010 - v1
Checksum:iA9670A30ACBB0FB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN557490 Genomic DNA. Translation: CBH27346.1.
RefSeqiYP_003464432.1. NC_013891.1.

Genome annotation databases

EnsemblBacteriaiCBH27346; CBH27346; lse_1195.
KEGGilsg:lse_1195.
PATRICi32262285. VBILisSee138575_1189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FN557490 Genomic DNA. Translation: CBH27346.1.
RefSeqiYP_003464432.1. NC_013891.1.

3D structure databases

ProteinModelPortaliD3UML3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBH27346; CBH27346; lse_1195.
KEGGilsg:lse_1195.
PATRICi32262285. VBILisSee138575_1189.

Phylogenomic databases

HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.

Enzyme and pathway databases

BioCyciLSEE683837:GI10-1218-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35967 / DSM 20751 / CIP 100100 / SLCC 3954Imported.

Entry informationi

Entry nameiD3UML3_LISSS
AccessioniPrimary (citable) accession number: D3UML3
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: April 1, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.