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Protein

ADP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

nnrD

Organism
Natrialba magadii (strain ATCC 43099 / DSM 3394 / CIP 104546 / JCM 8861/ NBRC 102185 / NCIMB 2190 / MS3) (Natronobacterium magadii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.UniRule annotation
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei202NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi192 – 201ADPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.136UniRule annotation)
Alternative name(s):
ADP-dependent NAD(P)HX dehydrataseUniRule annotation
Gene namesi
Name:nnrDUniRule annotation
Ordered Locus Names:Nmag_1014
OrganismiNatrialba magadii (strain ATCC 43099 / DSM 3394 / CIP 104546 / JCM 8861/ NBRC 102185 / NCIMB 2190 / MS3) (Natronobacterium magadii)
Taxonomic identifieri547559 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaNatrialbalesNatrialbaceaeNatrialba
Proteomesi
  • UP000001879 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004161541 – 258ADP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST258

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi547559.Nmag_1014.

Structurei

3D structure databases

ProteinModelPortaliD3SQW0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 258YjeF C-terminalUniRule annotationAdd BLAST258

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 151NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation
Contains 1 YjeF C-terminal domain.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00018. Archaea.
COG0063. LUCA.
HOGENOMiHOG000095375.
OMAiTAEYGPG.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3SQW0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRLQRTLSN ISEESDTDNG RVAVVGGSIE YPNQPALVGR AALRTGTDHV
60 70 80 90 100
RTLVPEALYA PVAGSSPNLL VSPYDGDQFD QDAAADAVDI CEWADALVIG
110 120 130 140 150
PGLVDAEADA IRDVLERTDI PVVVDALAIE PALESDLSRA VLTPSGSEDG
160 170 180 190 200
PIYEAYGSLR AFTEETGAVI TLTGGVDVIV TTGERLENDT GTSALTVAGT
210 220 230 240 250
GDTLAGITAS LLGQEMDRQD AAELGAWILG KSGELATAEY GPGVVATDVI

ECIPKTIR
Length:258
Mass (Da):26,710
Last modified:April 20, 2010 - v1
Checksum:iB84DFA7F0A8057D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001932 Genomic DNA. Translation: ADD04598.1.
RefSeqiWP_004216846.1. NZ_AOHS01000056.1.

Genome annotation databases

EnsemblBacteriaiADD04598; ADD04598; Nmag_1014.
GeneIDi8823845.
KEGGinmg:Nmag_1014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001932 Genomic DNA. Translation: ADD04598.1.
RefSeqiWP_004216846.1. NZ_AOHS01000056.1.

3D structure databases

ProteinModelPortaliD3SQW0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi547559.Nmag_1014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADD04598; ADD04598; Nmag_1014.
GeneIDi8823845.
KEGGinmg:Nmag_1014.

Phylogenomic databases

eggNOGiarCOG00018. Archaea.
COG0063. LUCA.
HOGENOMiHOG000095375.
OMAiTAEYGPG.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRD_NATMM
AccessioniPrimary (citable) accession number: D3SQW0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.