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D3SI12 (D3SI12_DEHSG) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenosylhomocysteinase HAMAP-Rule MF_00563 RuleBase RU000548
EC=3.3.1.1 HAMAP-Rule MF_00563 RuleBase RU000548
Alternative name(s):
S-adenosyl-L-homocysteine hydrolase HAMAP-Rule MF_00563
Gene names
Name:ahcY HAMAP-Rule MF_00563
Ordered Locus Names:DehalGT_0452
OrganismDehalococcoides sp. (strain GT) [Complete proteome] [HAMAP] EMBL ADC73819.1
Taxonomic identifier633145 [NCBI]
Taxonomic lineageBacteria › Chloroflexi › Dehalococcoidia › Dehalococcoidales › Dehalococcoidaceae › Dehalococcoides ›

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP-Rule MF_00563

Catalytic activity

S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP-Rule MF_00563 RuleBase RU000548

Cofactor

Binds 1 NAD per subunit By similarity. HAMAP-Rule MF_00563 RuleBase RU000548

Pathway

Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP-Rule MF_00563 RuleBase RU000548

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00563.

Sequence similarities

Belongs to the adenosylhomocysteinase family. HAMAP-Rule MF_00563 RuleBase RU004166

Ontologies

Keywords
   Biological processOne-carbon metabolism HAMAP-Rule MF_00563 RuleBase RU000548
   Cellular componentCytoplasm HAMAP-Rule MF_00563
   LigandNAD HAMAP-Rule MF_00563 RuleBase RU000548
   Molecular functionHydrolase HAMAP-Rule MF_00563 RuleBase RU000548 EMBL ADC73819.1
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processone-carbon metabolic process

Inferred from electronic annotation. Source: HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionadenosylhomocysteinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding151 – 1533NAD By similarity HAMAP-Rule MF_00563
Nucleotide binding214 – 2196NAD By similarity HAMAP-Rule MF_00563
Nucleotide binding293 – 2953NAD By similarity HAMAP-Rule MF_00563

Sites

Binding site541Substrate By similarity PIRSR PIRSR001109-1 HAMAP-Rule MF_00563
Binding site1251Substrate By similarity PIRSR PIRSR001109-1 HAMAP-Rule MF_00563
Binding site1501Substrate By similarity PIRSR PIRSR001109-1 HAMAP-Rule MF_00563
Binding site1801Substrate By similarity PIRSR PIRSR001109-1 HAMAP-Rule MF_00563
Binding site1841Substrate By similarity PIRSR PIRSR001109-1 HAMAP-Rule MF_00563
Binding site1851NAD By similarity HAMAP-Rule MF_00563
Binding site2371NAD By similarity HAMAP-Rule MF_00563
Binding site3401NAD By similarity HAMAP-Rule MF_00563

Sequences

Sequence LengthMass (Da)Tools
D3SI12 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 20C058AB41FCCC7D

FASTA41845,339
        10         20         30         40         50         60 
MPNFDVKDMS LAALGKKRVD WAGREMKTLS LIKERFEKEK PFKGIRIAAC LHITTETANL 

        70         80         90        100        110        120 
GLVLKAGGAD LTLCASNPLS TQDETAAALV EHGINTFAIH GENNESYYRH IAAALDAKPQ 

       130        140        150        160        170        180 
LTVDDGADLV ATLHSQRTEL LNGILGGTEE TTTGVIRLRA LADSGKLRYP IIAVNDAKTK 

       190        200        210        220        230        240 
YMFDNRYGTG QSTLDGITRA TNFLWASKTV VVVGYGWCGH GVAMRAKGMG AHIIVTEVDP 

       250        260        270        280        290        300 
VKALEAVMDG FSVMPMAEAA KLGDIFITVT GDKHVIDKDH FKVMKDGATL ANSGHFNSEI 

       310        320        330        340        350        360 
NIPALESLSC GKDTIRPSVE EYKMKDGRRI YLLGEGRLIN LAAAEGHPAA VMDMSFANQA 

       370        380        390        400        410 
LSLEYLVKNH TKLEKKVYAV PEEIDRAVAA TKLASLNISI DTLSAEQIKY LSSWQEGT

« Hide

References

[1]"Complete sequence of Dehalococcoides sp. GT."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Misra M., Chertkov O., Detter J.C., Han C., Tapia R., Kuske C.R., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G. expand/collapse author list , Ritalahti K.M., Fletcher K.E., Sung Y., Wagner D., Konstantinidis K.T., Loeffler F.E., Woyke T.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001924 Genomic DNA. Translation: ADC73819.1.
RefSeqYP_003462275.1. NC_013890.1.

3D structure databases

ProteinModelPortalD3SI12.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC73819; ADC73819; DehalGT_0452.
GeneID8808901.
KEGGdeg:DehalGT_0452.
PATRIC32054846. VBIDehSp13473_0423.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000227987.
KOK01251.

Enzyme and pathway databases

BioCycDSP633145:GHAK-459-MONOMER.
UniPathwayUPA00314; UER00076.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00563. AdoHcyase.
InterProIPR000043. Adenosylhomocysteinase.
IPR015878. Ado_hCys_hydrolase_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23420. PTHR23420. 1 hit.
PfamPF05221. AdoHcyase. 1 hit.
PF00670. AdoHcyase_NAD. 1 hit.
[Graphical view]
PIRSFPIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMARTSM00996. AdoHcyase. 1 hit.
SM00997. AdoHcyase_NAD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00936. ahcY. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD3SI12_DEHSG
AccessionPrimary (citable) accession number: D3SI12
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 29, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info