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D3SD89 (D3SD89_THISK) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:TK90_2198 EMBL ADC72688.1
OrganismThioalkalivibrio sp. (strain K90mix) [Complete proteome] [HAMAP] EMBL ADC72688.1
Taxonomic identifier396595 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaChromatialesEctothiorhodospiraceaeThioalkalivibrio

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain108 – 304197ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding134 – 18956ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2581Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2711Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2731Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D3SD89 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: 6AFF6C76C890E07B

FASTA31333,395
        10         20         30         40         50         60 
MSRRKPEEYG RVAVLMGGWS GERDISLKSG NAVLAALRRQ GVDAHAVDLT RETARCMALE 

        70         80         90        100        110        120 
GYDRVFIALH GECGEDGTLQ GCLDMGGMPY TGTGVLGSAL GMDKLACKRL WAGTNLPSAP 

       130        140        150        160        170        180 
YRLLTPGFDP EAVADALGLP LIVKPARGGS SLGITRVDRV ADLPAAFEAA RVQPGQVFAE 

       190        200        210        220        230        240 
QWITGREFTV AVLGNEPLPV IEIRAASGFY DYHAKYEADD TGYLCPPPDL SRSAQLELQS 

       250        260        270        280        290        300 
LAIAAFAALE GCGWGRVDIL QDEHGENFLI EVNTIPGMTG HSLVPKAAAQ AGIDFDELCL 

       310 
RILDSSFECG GLR 

« Hide

References

[1]"Complete sequence of chromosome of Thioalkalivibrio sp. K90mix."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Foster B., Sun H., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Sorokin D.Y., Muyzer G., Woyke T.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K90mix.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001905 Genomic DNA. Translation: ADC72688.1.
RefSeqYP_003461424.1. NC_013889.1.

3D structure databases

ProteinModelPortalD3SD89.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC72688; ADC72688; TK90_2198.
GeneID8807977.
KEGGtkm:TK90_2198.
PATRIC32527988. VBIThiSp13812_2186.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011592.
KOK01921.

Enzyme and pathway databases

BioCycTSP396595:GH8D-2249-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD3SD89_THISK
AccessionPrimary (citable) accession number: D3SD89
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: June 11, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)