D3SAD0 (D3SAD0_THISK) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ribonuclease HII HAMAP-Rule MF_00052
Short name=RNase HII HAMAP-Rule MF_00052
EC=3.1.26.4 HAMAP-Rule MF_00052

Gene names

Name:	rnhB HAMAP-Rule MF_00052
Ordered Locus Names:	TK90_1481 EMBL ADC71985.1

Organism

Thioalkalivibrio sp. (strain K90mix) [Complete proteome] [HAMAP] EMBL ADC71985.1

Taxonomic identifier

396595 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Chromatiales › Ectothiorhodospiraceae › Thioalkalivibrio

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. RuleBase RU003515 SAAS SAAS022898 HAMAP-Rule MF_00052
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. RuleBase RU003515 SAAS SAAS022898 HAMAP-Rule MF_00052
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity. SAAS SAAS022898 HAMAP-Rule MF_00052
Subcellular location	Cytoplasm By similarity SAAS SAAS022898 HAMAP-Rule MF_00052.
Sequence similarities	Belongs to the RNase HII family. RuleBase RU003515 HAMAP-Rule MF_00052

Ontologies

Keywords
Cellular component	Cytoplasm SAAS SAAS022898 HAMAP-Rule MF_00052
Ligand	Manganese SAAS SAAS022898 HAMAP-Rule MF_00052 Metal-binding SAAS SAAS022898 HAMAP-Rule MF_00052
Molecular function	Endonuclease RuleBase RU003515 SAAS SAAS022898 HAMAP-Rule MF_00052 Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: HAMAP ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	40	1	Divalent metal cation By similarity HAMAP-Rule MF_00052
	Metal binding	41	1	Divalent metal cation By similarity HAMAP-Rule MF_00052
	Metal binding	132	1	Divalent metal cation By similarity HAMAP-Rule MF_00052

Sequences

	Sequence		Length	Mass (Da)
	D3SAD0 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: 614D5720C0BC5447	FASTA	226	23,683
	10 20 30 40 50 60 MSAIHSRYCP GKHGFQCFLN PAGGSMSANR VTNAWVAGVD EVGRGPLAGP VVAAAVILPE 70 80 90 100 110 120 SFAAPGLTDS KKLSAGRRQA LDVEIRERAV AWALGRAEAA EIDALNIHRA TLLAMQRAVA 130 140 150 160 170 180 ALSQAPDHLL VDGRFTPEGP WSAEAIVGGD GSVAAISAAS VIAKVARDAE MTALHERYPD 190 200 210 220 YGFDRHAGYP TAYHRDALAR LGPCVEHRRS FGPVARALAA VAGEEA

References

[1]

"Complete sequence of chromosome of Thioalkalivibrio sp. K90mix." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Foster B., Sun H., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Sorokin D.Y., Muyzer G., Woyke T. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K90mix.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001905 Genomic DNA. Translation: ADC71985.1.
RefSeq	YP_003460721.1. NC_013889.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADC71985; ADC71985; TK90_1481.
GeneID	8807247.
KEGG	tkm:TK90_1481.
PATRIC	32526530. VBIThiSp13812_1469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000100288.
KO	K03470.
Enzyme and pathway databases
BioCyc	TSP396595:GH8D-1519-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B.
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
PANTHER	PTHR10954. PTHR10954. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D3SAD0_THISK

Accession

Primary (citable) accession number: D3SAD0

Entry history

Integrated into UniProtKB/TrEMBL:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 29, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)