D3S447 (D3S447_METSF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase HAMAP-Rule MF_02008
EC=6.1.1.1 HAMAP-Rule MF_02008

Alternative name(s):
Tyrosyl-tRNA synthetase HAMAP-Rule MF_02008

Gene names

Name:	tyrS HAMAP-Rule MF_02008
Ordered Locus Names:	MFS40622_0930

Organism

Methanocaldococcus sp. (strain FS406-22) [Complete proteome] [HAMAP]

Taxonomic identifier

644281 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanocaldococcaceae › Methanocaldococcus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP-Rule MF_02008
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP-Rule MF_02008
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_02008
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_02008.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily. HAMAP-Rule MF_02008

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_02008
Cellular component	Cytoplasm HAMAP-Rule MF_02008
Ligand	ATP-binding HAMAP-Rule MF_02008 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_02008 EMBL ADC69611.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	32 – 40	9	"HIGH" region By similarity HAMAP-Rule MF_02008
	Motif	199 – 203	5	"KMSKS" region By similarity HAMAP-Rule MF_02008
Sites
	Binding site	27	1	Tyrosine By similarity HAMAP-Rule MF_02008
	Binding site	146	1	Tyrosine By similarity HAMAP-Rule MF_02008
	Binding site	150	1	Tyrosine By similarity HAMAP-Rule MF_02008
	Binding site	153	1	Tyrosine By similarity HAMAP-Rule MF_02008
	Binding site	168	1	Tyrosine By similarity HAMAP-Rule MF_02008
	Binding site	202	1	ATP By similarity HAMAP-Rule MF_02008

Sequences

	Sequence		Length	Mass (Da)
	D3S447 [UniParc]. Last modified April 20, 2010. Version 1. Checksum: DEE921DA90BF9576	FASTA	301	34,342
	10 20 30 40 50 60 MIKRNTSEII SEEELKEVLT KDEKSAYIGF EPSGKIHLGH YLQIKKMIDL QNAGFDIIIL 70 80 90 100 110 120 LADLHAYLNQ KGELDEIRKI GEYNKRVFEA MGLKAKYVYG SEFQLDKDYT LNVYKLALKT 130 140 150 160 170 180 TLKRARRSME LIAREDENPK VAEVIYPIMQ VNDIHYLGVD VAVGGMEQRK IHMLARELLP 190 200 210 220 230 240 KKVVCIHNPV LTGLDGEGKM SSSKGNFIAV DDSPEEIKAK IKKAYCPAGV VEGNPIMEIA 250 260 270 280 290 300 KYFLEYPLTI NRPEKYGGDL VVNSYEELET LFKNKELHPM DLKNAVAEEL IKILEPIRKR L

References

[1]

"Complete sequence of chromosome of Methanocaldococcus sp. FS406-22." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Teshima H., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Leigh J., Whitman W.B., Woyke T. Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FS406-22.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001901 Genomic DNA. Translation: ADC69611.1.
RefSeq	YP_003458347.1. NC_013887.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADC69611; ADC69611; MFS40622_0930.
GeneID	8804775.
KEGG	mfs:MFS40622_0930.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000228238.
KO	K01866.
OMA	KIHMLAR.
Enzyme and pathway databases
BioCyc	MSP644281:GI4H-955-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_02008. Tyr_tRNA_synth_type3.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002307. Tyr-tRNA-ligase. IPR023684. Tyr-tRNA-ligase_3. IPR023617. Tyr-tRNA-ligase_arc/euk-type. [Graphical view]
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PIRSF	PIRSF006588. TyrRS_arch_euk. 1 hit.
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D3S447_METSF

Accession

Primary (citable) accession number: D3S447

Entry history

Integrated into UniProtKB/TrEMBL:	April 20, 2010
Last sequence update:	April 20, 2010
Last modified:	May 1, 2013
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)