Skip Header

Contribute Send feedback
Read comments (?) or add your own

D3RWK7 (D3RWK7_FERPA) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
NAD-dependent protein deacylase HAMAP-Rule MF_01121
EC=3.5.1.- HAMAP-Rule MF_01121
Alternative name(s):
Regulatory protein SIR2 homolog HAMAP-Rule MF_01121
Gene names
Name:cobB HAMAP-Rule MF_01121
Ordered Locus Names:Ferp_0700 EMBL ADC64870.1
OrganismFerroglobus placidus (strain DSM 10642 / AEDII12DO) [Complete proteome] [HAMAP] EMBL ADC64870.1
Taxonomic identifier589924 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Archaeoglobi › Archaeoglobales › Archaeoglobaceae › Ferroglobus ›

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NAD-dependent protein deacetylase which modulates the activities of several enzymes which are inactive in their acetylated form. Deacetylates the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription. May also have NAD-dependent lysine demalonylase and desuccinylase activity By similarity. HAMAP-Rule MF_01121

Catalytic activity

NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. HAMAP-Rule MF_01121

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01121.

Domain

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-61 and Arg-64) that bind to malonylated and succinylated substrates and define the specificity By similarity. HAMAP-Rule MF_01121

Sequence similarities

Belongs to the sirtuin family. Class III subfamily. HAMAP-Rule MF_01121

Contains 1 deacetylase sirtuin-type domain. HAMAP-Rule MF_01121

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding17 – 3620NAD By similarity HAMAP-Rule MF_01121
Nucleotide binding95 – 984NAD By similarity HAMAP-Rule MF_01121
Nucleotide binding176 – 1783NAD By similarity HAMAP-Rule MF_01121
Nucleotide binding202 – 2043NAD By similarity HAMAP-Rule MF_01121

Sites

Active site1131Proton acceptor By similarity HAMAP-Rule MF_01121
Metal binding1241Zinc By similarity HAMAP-Rule MF_01121
Metal binding1401Zinc By similarity HAMAP-Rule MF_01121
Binding site611Substrate By similarity HAMAP-Rule MF_01121
Binding site641Substrate By similarity HAMAP-Rule MF_01121
Binding site2201NAD; via amide nitrogen By similarity HAMAP-Rule MF_01121

Sequences

Sequence LengthMass (Da)Tools
D3RWK7 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: CFE64B45F4CA2C08

FASTA23526,499
        10         20         30         40         50         60 
MKLWEFLKDN EVVVLTGAGI SADSGIPTFR GKDGLWNKYK PEELATPEAF RRNPKLVWEW 

        70         80         90        100        110        120 
YMWRMEKVFN AKPNKAHLAL AKLEEMGIVK AVITQNVDNL HERAGSKNVI HLHGRIDEAR 

       130        140        150        160        170        180 
CEVCGRIVRF EKPIKEIPYC CSMMRPNVVW FGEALPEDAL KKSIELVEKY DVLVVGTSGV 

       190        200        210        220        230 
VQPAASLPII AKKAGNKVAE VNVEESAITP IVDIFVRGRA SEVFEEILRI IEQTS

« Hide

References

[1]"Complete sequence of Ferroglobus placidus DSM 10642."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Holmes D. expand/collapse author list , Lovley D., Kyrpides N., Anderson I.J., Woyke T.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 10642 / AEDII12DO.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001899 Genomic DNA. Translation: ADC64870.1.
RefSeqYP_003435145.1. NC_013849.1.

3D structure databases

ProteinModelPortalD3RWK7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC64870; ADC64870; Ferp_0700.
GeneID8778204.
KEGGfpl:Ferp_0700.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000085950.
KOK12410.
OMAVLHMHGE.

Enzyme and pathway databases

BioCycFPLA589924:GHX0-714-MONOMER.

Family and domain databases

Gene3D3.30.1600.10. 2 hits.
HAMAPMF_01121. Sirtuin_ClassIII.
InterProIPR003000. Sirtuin.
IPR026591. Sirtuin_cat_small_dom.
IPR027546. Sirtuin_ClassIII.
IPR026590. Ssirtuin_cat_dom.
[Graphical view]
PANTHERPTHR11085. PTHR11085. 1 hit.
PfamPF02146. SIR2. 1 hit.
[Graphical view]
PROSITEPS50305. SIRTUIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD3RWK7_FERPA
AccessionPrimary (citable) accession number: D3RWK7
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 29, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info