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D3RHF9

- D3RHF9_KLEVT

UniProt

D3RHF9 - D3RHF9_KLEVT

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Protein

ATP-dependent 6-phosphofructokinase

Gene
pfkA, Kvar_4995
Organism
Klebsiella variicola (strain At-22)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi104 – 1041Magnesium; catalytic By similarityUniRule annotation
Active sitei128 – 1281Proton acceptor By similarityUniRule annotation
Binding sitei155 – 1551Allosteric activator ADP By similarityUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei212 – 2121Allosteric activator ADP By similarityUniRule annotation
Binding sitei223 – 2231Substrate By similarityUniRule annotation
Binding sitei244 – 2441Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATP By similarityUniRule annotation
Nucleotide bindingi103 – 1064ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciKVAR640131:GHXG-5104-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:Kvar_4995Imported
OrganismiKlebsiella variicola (strain At-22)Imported
Taxonomic identifieri640131 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
ProteomesiUP000001907: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

PTM / Processingi

Proteomic databases

PRIDEiD3RHF9.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD3RHF9.
SMRiD3RHF9. Positions 1-320.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni126 – 1283Substrate binding By similarityUniRule annotation
Regioni170 – 1723Substrate binding By similarityUniRule annotation
Regioni186 – 1883Allosteric activator ADP binding By similarityUniRule annotation
Regioni214 – 2163Allosteric activator ADP binding By similarityUniRule annotation
Regioni250 – 2534Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiGFGGRCV.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3RHF9-1 [UniParc]FASTAAdd to Basket

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MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVFGIYD GYLGLYEDRM    50
VQLDRYSVSD MINRGGTFLG SARFPEFREE HIRAVAIENM KKRGLDALVV 100
IGGDGSYMGA MRLTEMGFPC IGLPGTIDND IKGTDYTIGF FTALSTVVEA 150
IDRLRDTSSS HQRISVVEVM GRYCGDLTLA AAIAGGCEFI MVPEVEYTRD 200
DLVAEIKAGI AKGKKHAIVA ITEHMCDVDE LASYIEKETG RETRATVLGH 250
IQRGGSPVPY DRILASRMGA YAIELLLQGH GGRCVGIQNE KLVHHDIIDA 300
IENMKRPFKN DWLDCAKKLY 320
Length:320
Mass (Da):34,984
Last modified:April 20, 2010 - v1
Checksum:i07544D8598DF1604
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001891 Genomic DNA. Translation: ADC60866.1.
RefSeqiYP_003441898.1. NC_013850.1.

Genome annotation databases

EnsemblBacteriaiADC60866; ADC60866; Kvar_4995.
GeneIDi8785216.
KEGGikva:Kvar_4995.
PATRICi32230182. VBIKleVar54872_4966.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001891 Genomic DNA. Translation: ADC60866.1 .
RefSeqi YP_003441898.1. NC_013850.1.

3D structure databases

ProteinModelPortali D3RHF9.
SMRi D3RHF9. Positions 1-320.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi D3RHF9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADC60866 ; ADC60866 ; Kvar_4995 .
GeneIDi 8785216.
KEGGi kva:Kvar_4995.
PATRICi 32230182. VBIKleVar54872_4966.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi GFGGRCV.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci KVAR640131:GHXG-5104-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: At-22.

Entry informationi

Entry nameiD3RHF9_KLEVT
AccessioniPrimary (citable) accession number: D3RHF9
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: July 9, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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