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D3RHF9

- D3RHF9_KLEVT

UniProt

D3RHF9 - D3RHF9_KLEVT

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Klebsiella variicola (strain At-22)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 30 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei244 – 2441Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742ATPUniRule annotation
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciKVAR640131:GHXG-5104-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Kvar_4995Imported
    OrganismiKlebsiella variicola (strain At-22)Imported
    Taxonomic identifieri640131 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella
    ProteomesiUP000001907: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    PTM / Processingi

    Proteomic databases

    PRIDEiD3RHF9.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD3RHF9.
    SMRiD3RHF9. Positions 1-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
    Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
    Regioni250 – 2534Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGFGGRCV.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3RHF9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVFGIYD GYLGLYEDRM    50
    VQLDRYSVSD MINRGGTFLG SARFPEFREE HIRAVAIENM KKRGLDALVV 100
    IGGDGSYMGA MRLTEMGFPC IGLPGTIDND IKGTDYTIGF FTALSTVVEA 150
    IDRLRDTSSS HQRISVVEVM GRYCGDLTLA AAIAGGCEFI MVPEVEYTRD 200
    DLVAEIKAGI AKGKKHAIVA ITEHMCDVDE LASYIEKETG RETRATVLGH 250
    IQRGGSPVPY DRILASRMGA YAIELLLQGH GGRCVGIQNE KLVHHDIIDA 300
    IENMKRPFKN DWLDCAKKLY 320
    Length:320
    Mass (Da):34,984
    Last modified:April 20, 2010 - v1
    Checksum:i07544D8598DF1604
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001891 Genomic DNA. Translation: ADC60866.1.
    RefSeqiYP_003441898.1. NC_013850.1.

    Genome annotation databases

    EnsemblBacteriaiADC60866; ADC60866; Kvar_4995.
    GeneIDi8785216.
    KEGGikva:Kvar_4995.
    PATRICi32230182. VBIKleVar54872_4966.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001891 Genomic DNA. Translation: ADC60866.1 .
    RefSeqi YP_003441898.1. NC_013850.1.

    3D structure databases

    ProteinModelPortali D3RHF9.
    SMRi D3RHF9. Positions 1-320.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi D3RHF9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADC60866 ; ADC60866 ; Kvar_4995 .
    GeneIDi 8785216.
    KEGGi kva:Kvar_4995.
    PATRICi 32230182. VBIKleVar54872_4966.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GFGGRCV.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci KVAR640131:GHXG-5104-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: At-22Imported.

    Entry informationi

    Entry nameiD3RHF9_KLEVT
    AccessioniPrimary (citable) accession number: D3RHF9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 30 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteomeImported

    External Data

    Dasty 3