Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

D3RA30 (D3RA30_KLEVT) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Kvar_4058 EMBL ADC59937.1
OrganismKlebsiella variicola (strain At-22) [Complete proteome] [HAMAP] EMBL ADC59937.1
Taxonomic identifier640131 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 348204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding175 – 23056ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3171Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
D3RA30 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: FB60E554E248DF59

FASTA36539,445
        10         20         30         40         50         60 
MAKMRVGIVF GGKSAEHEVS LQSAKNIVEA IDKSRFDVVL LGIDKQGLWH INDAGNYLLN 

        70         80         90        100        110        120 
AQDPARIALR PSTVTLAQIP GREAQQLINA DSGQPLAAID VIFPIVHGTL GEDGSLQGML 

       130        140        150        160        170        180 
RMANLPFVGS DVLGSAACMD KDVTKRLLRD AGLAVAPFIT LTRANRAQFS FADVEAKLGL 

       190        200        210        220        230        240 
PLFVKPANQG SSVGISKVKN EEQYHQAVAL AFEFDHKVVV EQGIKGREIE CAVLGNDHPQ 

       250        260        270        280        290        300 
ASTCGEIVLN SEFYAYDTKY IDDQGAQVVV PAAIAPEIND KIRAIAVQAY QTLGCSGMAR 

       310        320        330        340        350        360 
VDVFLTADNE VVINEINTLP GFTNISMYPK LWQASGLDYT SLITRLIELA LERHAADRAL 


KTSMN 

« Hide

References

[1]"Complete sequence of Klebsiella variicola At-22."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., Pitluck S., Davenport K., Brettin T., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Pinto A., Currie C., Woyke T.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: At-22.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001891 Genomic DNA. Translation: ADC59937.1.
RefSeqYP_003440969.1. NC_013850.1.

3D structure databases

ProteinModelPortalD3RA30.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC59937; ADC59937; Kvar_4058.
GeneID8784236.
KEGGkva:Kvar_4058.
PATRIC32228198. VBIKleVar54872_4019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.

Enzyme and pathway databases

BioCycKVAR640131:GHXG-4124-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD3RA30_KLEVT
AccessionPrimary (citable) accession number: D3RA30
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: February 19, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)