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Protein

Ribosomal protein S12 methylthiotransferase RimO

Gene

rimO

Organism
Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.UniRule annotationSAAS annotation

Catalytic activityi

L-aspartate-[ribosomal protein S12] + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 3-methylthio-L-aspartate-[ribosomal protein S12] + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi77Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi143Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi147Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi150Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal proteinImported, TransferaseUniRule annotationSAAS annotationImported
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal protein S12 methylthiotransferase RimOUniRule annotationSAAS annotation (EC:2.8.4.4UniRule annotationSAAS annotation)
Short name:
S12 MTTaseUniRule annotation
Short name:
S12 methylthiotransferaseUniRule annotation
Alternative name(s):
Ribosomal protein S12 (aspartate-C(3))-methylthiotransferaseUniRule annotation
Ribosome maturation factor RimOUniRule annotation
Gene namesi
Name:rimOUniRule annotationImported
ORF Names:K649_04960Imported
OrganismiMeiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)Imported
Taxonomic identifieri504728 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
Proteomesi
  • UP000013026 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi504728.Mrub_1063

Structurei

3D structure databases

ProteinModelPortaliD3PQU4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 113MTTase N-terminalInterPro annotationAdd BLAST111
Domaini369 – 437TRAMInterPro annotationAdd BLAST69

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili349 – 369Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the methylthiotransferase family. RimO subfamily.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105CBM Bacteria
COG0621 LUCA
HOGENOMiHOG000224766
KOiK14441
OMAiCLSERYK

Family and domain databases

Gene3Di3.40.50.12160, 1 hit
3.80.30.20, 1 hit
HAMAPiMF_01865 MTTase_RimO, 1 hit
InterProiView protein in InterPro
IPR006638 Elp3/MiaB/NifB
IPR005839 Methylthiotransferase
IPR020612 Methylthiotransferase_CS
IPR013848 Methylthiotransferase_N
IPR038135 Methylthiotransferase_N_sf
IPR005840 Ribosomal_S12_MeSTrfase_RimO
IPR007197 rSAM
IPR023404 rSAM_horseshoe
IPR002792 TRAM_dom
PANTHERiPTHR43837 PTHR43837, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PF01938 TRAM, 1 hit
PF00919 UPF0004, 1 hit
SFLDiSFLDF00274 ribosomal_protein_S12_methylth, 1 hit
SFLDG01061 methylthiotransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00089 TIGR00089, 1 hit
TIGR01125 TIGR01125, 1 hit
PROSITEiView protein in PROSITE
PS51449 MTTASE_N, 1 hit
PS01278 MTTASE_RADICAL, 1 hit
PS50926 TRAM, 1 hit

Sequencei

Sequence statusi: Complete.

D3PQU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGKVGFVSL GCPKALVDSE QILSRLRAQG YETSPTYQEA DVVVVNTCGF
60 70 80 90 100
ITPAVEESLT AIGEALSENG KVIVTGCLGA RPEVIQQAHP QVLEVTGPGE
110 120 130 140 150
VDRVLAAVQR VVPLEQNPFT ALVPPQVKLT PRHYAYLKIA EGCNHKCSFC
160 170 180 190 200
IIPKLRGLQQ SRDAAEILFE ATRLVGTGTK ELLVIAQDTS AYGVDIRHRT
210 220 230 240 250
SDYAGKQVRA HLVDLVNELA GLGAWLRLHY VYPYPHVRDL IPLMAEGKLL
260 270 280 290 300
PYLDVPLQHA SPRVLRAMRR PGGAESHLKT IQEWRAIAPD LAIRSSFIVG
310 320 330 340 350
FPGETEEDFE LLLDFIAEAR LDRVGCFTYS EVEGADANSL PGAVPQEVKE
360 370 380 390 400
ERRARLMALQ QQISLEKNQA RLGQTLEVIV DDYGELPGLV VGRSRYDAPG
410 420 430 440 450
IDGLVYAETD GTVKIGDIIQ VRITQAEAYD LHGEMVGRVA WKPAVPVIKN

VATETIQV
Length:458
Mass (Da):50,106
Last modified:April 20, 2010 - v1
Checksum:iB5CBD124ED5BC1F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP005385 Genomic DNA Translation: AGK04294.1
RefSeqiWP_013013346.1, NC_021081.1

Genome annotation databases

EnsemblBacteriaiAGK04294; AGK04294; K649_04960
KEGGimrb:Mrub_1063
mre:K649_04960
PATRICifig|504728.9.peg.1023

Similar proteinsi

Entry informationi

Entry nameiD3PQU4_MEIRD
AccessioniPrimary (citable) accession number: D3PQU4
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: March 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health