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D3PQ55

- D3PQ55_MEIRD

UniProt

D3PQ55 - D3PQ55_MEIRD

Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (20 Apr 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

    Cofactori

    Binds 4 magnesium or manganese ions per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi286 – 2861Magnesium or manganese 1UniRule annotation
    Metal bindingi300 – 3001Magnesium or manganese 1UniRule annotation
    Metal bindingi300 – 3001Magnesium or manganese 2UniRule annotation
    Metal bindingi302 – 3021Magnesium or manganese 2UniRule annotation
    Metal bindingi843 – 8431Magnesium or manganese 3UniRule annotation
    Metal bindingi855 – 8551Magnesium or manganese 3UniRule annotation
    Metal bindingi855 – 8551Magnesium or manganese 4UniRule annotation
    Metal bindingi857 – 8571Magnesium or manganese 4UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi160 – 21758ATPUniRule annotationAdd
    BLAST
    Nucleotide bindingi718 – 77659ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    2. arginine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    LigaseUniRule annotationImported

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMRUB504728:GI6U-937-MONOMER.
    UniPathwayiUPA00068; UER00171.
    UPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
    Alternative name(s):
    Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
    Gene namesi
    Name:carBUniRule annotationImported
    Ordered Locus Names:Mrub_0916Imported
    ORF Names:K649_04220Imported
    OrganismiMeiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)Imported
    Taxonomic identifieri504728 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
    ProteomesiUP000006655: Chromosome, UP000013026: Chromosome

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini133 – 329197ATP-grasp 1UniRule annotationAdd
    BLAST
    Domaini692 – 884193ATP-grasp 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 411411Carboxyphosphate synthetic domainUniRule annotationAdd
    BLAST
    Regioni412 – 554143Oligomerization domainUniRule annotationAdd
    BLAST
    Regioni555 – 950396Carbamoyl phosphate synthetic domainUniRule annotationAdd
    BLAST
    Regioni951 – 103888Allosteric domainUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarB family.UniRule annotation
    Contains 2 ATP-grasp domains.UniRule annotation

    Keywords - Domaini

    RepeatUniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000234582.
    KOiK01955.
    OMAiGSDRIWY.

    Family and domain databases

    Gene3Di1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.20. 2 hits.
    HAMAPiMF_01210_B. CPSase_L_chain_B.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    [Graphical view]
    PRINTSiPR00098. CPSASE.
    SMARTiSM01096. CPSase_L_D3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48108. SSF48108. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3PQ55-1 [UniParc]FASTAAdd to Basket

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    MPARTDLKKI MIIGSGPITI GQAAEFDYSG TQAVKALRSV GYQVVLVNSN     50
    PATIMTDPEL AEGTYLEPLT VEFLEKIIAA ERPDALLPTL GGQTALNLSM 100
    ALYEQGILEK YGVELIGANA AAIKKGEDRE EFQKAMLKIG VDVPRGKMVH 150
    SLEEGLAFAR EVTGYPVVVR PSFTLGGTGG GIAENEAEFV EILSRGLSLS 200
    PTHSALVEES IVGWKEYELE VMRDKNDTVV IITSIENLDP MGVHTGDSIT 250
    VAPAQTLSDV EYQRMRDAAI AIIREIGVET GGSNIQFAID PKSGRMIVIE 300
    MNPRVSRSSA LASKATGFPI AKIAALLAVG YTLDEIPNDI TQKTPASFEP 350
    TIDYVVTKIP RFAFEKFSTL PNTNPGGFSD RLGTQMKSVG EVMAIGRTFK 400
    ESFGKALRSL EADVRSEFSG LTTEELEARL YPSPTRIYAV LELLRRGVQV 450
    DHLYEKTKIE PWFLTQLKEV VEAEHDLETE PHRLEDLEDW RYVKGLGLSD 500
    ARVGELLGVS EAAARKQRLA AGCKPVYKTV DTCAAEFEAY TPYHYSTYEL 550
    EDEVRPTSKP KVVILGSGPI RIGQGVEFDY ATVHAVWALR EAAIPPERPG 600
    EAGARGYETI MVNSNPETVS TDYDTADRLY FEPLTLEDVL NLAEHEKPIG 650
    VIATLGGQTP LKLAKKLAEA GVPLLGTSWE AIHKAEDRAE FNQLCAALGI 700
    PQPKGAVART PEEALKLAET LGYPLMARPS YVLGGRAMQV VRNPEELRWY 750
    LSGIYAALAE RPSILLDQYL ENALELDVDA LCDGKQVVVA GIMEHIERAG 800
    VHSGDSATIL PPISLTPEQL ETVKAYTRKL ALAVGVRGLI NVQYAIKDGV 850
    VYILEANPRA SRTVPFVSKA IGHPLAKYAA LIAVGHTLEE LGFTQDPTPS 900
    FYAVKEVLIP WLKFPGVIPV LGPEMRSTGE SMGIDTDPYL AYYRAELGVG 950
    QRLPLSGKVR FIGAEQLKPD WEAAGFEVSE GDYDLLIALT PDPELRRAVE 1000
    TGKPFITTLE GARWSLEAIL RARHSNLPVR SLQAWHGR 1038
    Length:1,038
    Mass (Da):113,358
    Last modified:April 20, 2010 - v1
    Checksum:iA3DA9A98A1E25318
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001743 Genomic DNA. Translation: ADD27681.1.
    CP005385 Genomic DNA. Translation: AGK04146.1.
    RefSeqiWP_013013200.1. NC_021081.1.
    YP_003506701.1. NC_013946.1.
    YP_007879643.1. NC_021081.1.

    Genome annotation databases

    EnsemblBacteriaiADD27681; ADD27681; Mrub_0916.
    AGK04146; AGK04146; K649_04220.
    GeneIDi15335456.
    8879003.
    KEGGimrb:Mrub_0916.
    mre:K649_04220.
    PATRICi35357964. VBIMeiRub128672_0920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001743 Genomic DNA. Translation: ADD27681.1 .
    CP005385 Genomic DNA. Translation: AGK04146.1 .
    RefSeqi WP_013013200.1. NC_021081.1.
    YP_003506701.1. NC_013946.1.
    YP_007879643.1. NC_021081.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADD27681 ; ADD27681 ; Mrub_0916 .
    AGK04146 ; AGK04146 ; K649_04220 .
    GeneIDi 15335456.
    8879003.
    KEGGi mrb:Mrub_0916.
    mre:K649_04220.
    PATRICi 35357964. VBIMeiRub128672_0920.

    Phylogenomic databases

    HOGENOMi HOG000234582.
    KOi K01955.
    OMAi GSDRIWY.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00171 .
    UPA00070 ; UER00115 .
    BioCyci MRUB504728:GI6U-937-MONOMER.

    Family and domain databases

    Gene3Di 1.10.1030.10. 1 hit.
    3.30.1490.20. 2 hits.
    3.30.470.20. 2 hits.
    3.40.50.20. 2 hits.
    HAMAPi MF_01210_B. CPSase_L_chain_B.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006275. CarbamoylP_synth_lsu.
    IPR005481. CarbamoylP_synth_lsu_N.
    IPR005480. CarbamoylP_synth_lsu_oligo.
    IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
    IPR005483. CbamoylP_synth_lsu_CPSase_dom.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF00289. CPSase_L_chain. 2 hits.
    PF02786. CPSase_L_D2. 2 hits.
    PF02787. CPSase_L_D3. 1 hit.
    [Graphical view ]
    PRINTSi PR00098. CPSASE.
    SMARTi SM01096. CPSase_L_D3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48108. SSF48108. 1 hit.
    SSF52440. SSF52440. 2 hits.
    TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 2 hits.
    PS00866. CPSASE_1. 1 hit.
    PS00867. CPSASE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 35948 / DSM 1279 / VKM B-1258 / 21Imported.
    2. "Non-Hybrid, Finished Microbial Genome Assemblies from Long-Read SMRT Sequencing Data."
      Klammer A., Drake J., Heiner C., Clum A., Copeland A., Huddleston J., Eichler E., Turner S.W.
      Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 1279Imported.
    3. Chin J., Alexander D.H., Marks P., Korlach J., Clum A., Copeland A.
      Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: DSM 1279Imported.

    Entry informationi

    Entry nameiD3PQ55_MEIRD
    AccessioniPrimary (citable) accession number: D3PQ55
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3