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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 4 magnesium or manganese ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Magnesium or manganese 1UniRule annotation
Metal bindingi300 – 3001Magnesium or manganese 1UniRule annotation
Metal bindingi300 – 3001Magnesium or manganese 2UniRule annotation
Metal bindingi302 – 3021Magnesium or manganese 2UniRule annotation
Metal bindingi843 – 8431Magnesium or manganese 3UniRule annotation
Metal bindingi855 – 8551Magnesium or manganese 3UniRule annotation
Metal bindingi855 – 8551Magnesium or manganese 4UniRule annotation
Metal bindingi857 – 8571Magnesium or manganese 4UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 21758ATPUniRule annotationAdd
BLAST
Nucleotide bindingi718 – 77659ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRUB504728:GI6U-937-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
ORF Names:K649_04220Imported
OrganismiMeiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)Imported
Taxonomic identifieri504728 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
ProteomesiUP000013026 Componenti: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD3PQ55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 329197ATP-grasp 1UniRule annotationAdd
BLAST
Domaini692 – 884193ATP-grasp 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 411411Carboxyphosphate synthetic domainUniRule annotationAdd
BLAST
Regioni412 – 554143Oligomerization domainUniRule annotationAdd
BLAST
Regioni555 – 950396Carbamoyl phosphate synthetic domainUniRule annotationAdd
BLAST
Regioni951 – 103888Allosteric domainUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation
Contains 2 ATP-grasp domains.UniRule annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3PQ55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARTDLKKI MIIGSGPITI GQAAEFDYSG TQAVKALRSV GYQVVLVNSN
60 70 80 90 100
PATIMTDPEL AEGTYLEPLT VEFLEKIIAA ERPDALLPTL GGQTALNLSM
110 120 130 140 150
ALYEQGILEK YGVELIGANA AAIKKGEDRE EFQKAMLKIG VDVPRGKMVH
160 170 180 190 200
SLEEGLAFAR EVTGYPVVVR PSFTLGGTGG GIAENEAEFV EILSRGLSLS
210 220 230 240 250
PTHSALVEES IVGWKEYELE VMRDKNDTVV IITSIENLDP MGVHTGDSIT
260 270 280 290 300
VAPAQTLSDV EYQRMRDAAI AIIREIGVET GGSNIQFAID PKSGRMIVIE
310 320 330 340 350
MNPRVSRSSA LASKATGFPI AKIAALLAVG YTLDEIPNDI TQKTPASFEP
360 370 380 390 400
TIDYVVTKIP RFAFEKFSTL PNTNPGGFSD RLGTQMKSVG EVMAIGRTFK
410 420 430 440 450
ESFGKALRSL EADVRSEFSG LTTEELEARL YPSPTRIYAV LELLRRGVQV
460 470 480 490 500
DHLYEKTKIE PWFLTQLKEV VEAEHDLETE PHRLEDLEDW RYVKGLGLSD
510 520 530 540 550
ARVGELLGVS EAAARKQRLA AGCKPVYKTV DTCAAEFEAY TPYHYSTYEL
560 570 580 590 600
EDEVRPTSKP KVVILGSGPI RIGQGVEFDY ATVHAVWALR EAAIPPERPG
610 620 630 640 650
EAGARGYETI MVNSNPETVS TDYDTADRLY FEPLTLEDVL NLAEHEKPIG
660 670 680 690 700
VIATLGGQTP LKLAKKLAEA GVPLLGTSWE AIHKAEDRAE FNQLCAALGI
710 720 730 740 750
PQPKGAVART PEEALKLAET LGYPLMARPS YVLGGRAMQV VRNPEELRWY
760 770 780 790 800
LSGIYAALAE RPSILLDQYL ENALELDVDA LCDGKQVVVA GIMEHIERAG
810 820 830 840 850
VHSGDSATIL PPISLTPEQL ETVKAYTRKL ALAVGVRGLI NVQYAIKDGV
860 870 880 890 900
VYILEANPRA SRTVPFVSKA IGHPLAKYAA LIAVGHTLEE LGFTQDPTPS
910 920 930 940 950
FYAVKEVLIP WLKFPGVIPV LGPEMRSTGE SMGIDTDPYL AYYRAELGVG
960 970 980 990 1000
QRLPLSGKVR FIGAEQLKPD WEAAGFEVSE GDYDLLIALT PDPELRRAVE
1010 1020 1030
TGKPFITTLE GARWSLEAIL RARHSNLPVR SLQAWHGR
Length:1,038
Mass (Da):113,358
Last modified:April 20, 2010 - v1
Checksum:iA3DA9A98A1E25318
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP005385 Genomic DNA. Translation: AGK04146.1.
RefSeqiWP_013013200.1. NC_021081.1.
YP_003506701.1. NC_013946.1.
YP_007879643.1. NC_021081.1.

Genome annotation databases

EnsemblBacteriaiADD27681; ADD27681; Mrub_0916.
AGK04146; AGK04146; K649_04220.
KEGGimrb:Mrub_0916.
mre:K649_04220.
PATRICi35357964. VBIMeiRub128672_0920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP005385 Genomic DNA. Translation: AGK04146.1.
RefSeqiWP_013013200.1. NC_021081.1.
YP_003506701.1. NC_013946.1.
YP_007879643.1. NC_021081.1.

3D structure databases

ProteinModelPortaliD3PQ55.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADD27681; ADD27681; Mrub_0916.
AGK04146; AGK04146; K649_04220.
KEGGimrb:Mrub_0916.
mre:K649_04220.
PATRICi35357964. VBIMeiRub128672_0920.

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiAVFPFNK.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.
BioCyciMRUB504728:GI6U-937-MONOMER.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Chin J., Alexander D.H., Marks P., Korlach J., Clum A., Copeland A.
    Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 1279Imported.

Entry informationi

Entry nameiD3PQ55_MEIRD
AccessioniPrimary (citable) accession number: D3PQ55
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 27, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.