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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase large chain (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRUB504728:G1HGZ-853-MONOMER
UniPathwayiUPA00068; UER00171
UPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
ORF Names:K649_04220Imported
OrganismiMeiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)Imported
Taxonomic identifieri504728 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
Proteomesi
  • UP000013026 Componenti: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi504728.Mrub_0916

Structurei

3D structure databases

ProteinModelPortaliD3PQ55
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 329ATP-graspInterPro annotationAdd BLAST197
Domaini692 – 884ATP-graspInterPro annotationAdd BLAST193

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 411Carboxyphosphate synthetic domainUniRule annotationAdd BLAST411
Regioni951 – 1038Allosteric domainUniRule annotationAdd BLAST88

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

eggNOGiENOG4105CU6 Bacteria
COG0458 LUCA
HOGENOMiHOG000234582
KOiK01955
OMAiAVFPFNK

Family and domain databases

Gene3Di1.10.1030.10, 1 hit
HAMAPiMF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00866 CPSASE_1, 1 hit
PS00867 CPSASE_2, 2 hits

Sequencei

Sequence statusi: Complete.

D3PQ55-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARTDLKKI MIIGSGPITI GQAAEFDYSG TQAVKALRSV GYQVVLVNSN
60 70 80 90 100
PATIMTDPEL AEGTYLEPLT VEFLEKIIAA ERPDALLPTL GGQTALNLSM
110 120 130 140 150
ALYEQGILEK YGVELIGANA AAIKKGEDRE EFQKAMLKIG VDVPRGKMVH
160 170 180 190 200
SLEEGLAFAR EVTGYPVVVR PSFTLGGTGG GIAENEAEFV EILSRGLSLS
210 220 230 240 250
PTHSALVEES IVGWKEYELE VMRDKNDTVV IITSIENLDP MGVHTGDSIT
260 270 280 290 300
VAPAQTLSDV EYQRMRDAAI AIIREIGVET GGSNIQFAID PKSGRMIVIE
310 320 330 340 350
MNPRVSRSSA LASKATGFPI AKIAALLAVG YTLDEIPNDI TQKTPASFEP
360 370 380 390 400
TIDYVVTKIP RFAFEKFSTL PNTNPGGFSD RLGTQMKSVG EVMAIGRTFK
410 420 430 440 450
ESFGKALRSL EADVRSEFSG LTTEELEARL YPSPTRIYAV LELLRRGVQV
460 470 480 490 500
DHLYEKTKIE PWFLTQLKEV VEAEHDLETE PHRLEDLEDW RYVKGLGLSD
510 520 530 540 550
ARVGELLGVS EAAARKQRLA AGCKPVYKTV DTCAAEFEAY TPYHYSTYEL
560 570 580 590 600
EDEVRPTSKP KVVILGSGPI RIGQGVEFDY ATVHAVWALR EAAIPPERPG
610 620 630 640 650
EAGARGYETI MVNSNPETVS TDYDTADRLY FEPLTLEDVL NLAEHEKPIG
660 670 680 690 700
VIATLGGQTP LKLAKKLAEA GVPLLGTSWE AIHKAEDRAE FNQLCAALGI
710 720 730 740 750
PQPKGAVART PEEALKLAET LGYPLMARPS YVLGGRAMQV VRNPEELRWY
760 770 780 790 800
LSGIYAALAE RPSILLDQYL ENALELDVDA LCDGKQVVVA GIMEHIERAG
810 820 830 840 850
VHSGDSATIL PPISLTPEQL ETVKAYTRKL ALAVGVRGLI NVQYAIKDGV
860 870 880 890 900
VYILEANPRA SRTVPFVSKA IGHPLAKYAA LIAVGHTLEE LGFTQDPTPS
910 920 930 940 950
FYAVKEVLIP WLKFPGVIPV LGPEMRSTGE SMGIDTDPYL AYYRAELGVG
960 970 980 990 1000
QRLPLSGKVR FIGAEQLKPD WEAAGFEVSE GDYDLLIALT PDPELRRAVE
1010 1020 1030
TGKPFITTLE GARWSLEAIL RARHSNLPVR SLQAWHGR
Length:1,038
Mass (Da):113,358
Last modified:April 20, 2010 - v1
Checksum:iA3DA9A98A1E25318
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP005385 Genomic DNA Translation: AGK04146.1
RefSeqiWP_013013200.1, NC_021081.1

Genome annotation databases

EnsemblBacteriaiAGK04146; AGK04146; K649_04220
KEGGimrb:Mrub_0916
mre:K649_04220
PATRICifig|504728.9.peg.873

Similar proteinsi

Entry informationi

Entry nameiD3PQ55_MEIRD
AccessioniPrimary (citable) accession number: D3PQ55
Entry historyiIntegrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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