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D3PQ55

- D3PQ55_MEIRD

UniProt

D3PQ55 - D3PQ55_MEIRD

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Protein

Carbamoyl-phosphate synthase large chain

Gene

carB

Organism
Meiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation

Cofactori

Binds 4 magnesium or manganese ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Magnesium or manganese 1UniRule annotation
Metal bindingi300 – 3001Magnesium or manganese 1UniRule annotation
Metal bindingi300 – 3001Magnesium or manganese 2UniRule annotation
Metal bindingi302 – 3021Magnesium or manganese 2UniRule annotation
Metal bindingi843 – 8431Magnesium or manganese 3UniRule annotation
Metal bindingi855 – 8551Magnesium or manganese 3UniRule annotation
Metal bindingi855 – 8551Magnesium or manganese 4UniRule annotation
Metal bindingi857 – 8571Magnesium or manganese 4UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi160 – 21758ATPUniRule annotationAdd
BLAST
Nucleotide bindingi718 – 77659ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMRUB504728:GI6U-937-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotationImported
Ordered Locus Names:Mrub_0916Imported
ORF Names:K649_04220Imported
OrganismiMeiothermus ruber (strain ATCC 35948 / DSM 1279 / VKM B-1258 / 21) (Thermus ruber)Imported
Taxonomic identifieri504728 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMeiothermus
ProteomesiUP000006655: Chromosome, UP000013026: Chromosome

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD3PQ55.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 329197ATP-grasp 1UniRule annotationAdd
BLAST
Domaini692 – 884193ATP-grasp 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 411411Carboxyphosphate synthetic domainUniRule annotationAdd
BLAST
Regioni412 – 554143Oligomerization domainUniRule annotationAdd
BLAST
Regioni555 – 950396Carbamoyl phosphate synthetic domainUniRule annotationAdd
BLAST
Regioni951 – 103888Allosteric domainUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CarB family.UniRule annotation
Contains 2 ATP-grasp domains.UniRule annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

HOGENOMiHOG000234582.
KOiK01955.
OMAiGSDRIWY.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.20. 2 hits.
HAMAPiMF_01210_B. CPSase_L_chain_B.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3PQ55-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPARTDLKKI MIIGSGPITI GQAAEFDYSG TQAVKALRSV GYQVVLVNSN
60 70 80 90 100
PATIMTDPEL AEGTYLEPLT VEFLEKIIAA ERPDALLPTL GGQTALNLSM
110 120 130 140 150
ALYEQGILEK YGVELIGANA AAIKKGEDRE EFQKAMLKIG VDVPRGKMVH
160 170 180 190 200
SLEEGLAFAR EVTGYPVVVR PSFTLGGTGG GIAENEAEFV EILSRGLSLS
210 220 230 240 250
PTHSALVEES IVGWKEYELE VMRDKNDTVV IITSIENLDP MGVHTGDSIT
260 270 280 290 300
VAPAQTLSDV EYQRMRDAAI AIIREIGVET GGSNIQFAID PKSGRMIVIE
310 320 330 340 350
MNPRVSRSSA LASKATGFPI AKIAALLAVG YTLDEIPNDI TQKTPASFEP
360 370 380 390 400
TIDYVVTKIP RFAFEKFSTL PNTNPGGFSD RLGTQMKSVG EVMAIGRTFK
410 420 430 440 450
ESFGKALRSL EADVRSEFSG LTTEELEARL YPSPTRIYAV LELLRRGVQV
460 470 480 490 500
DHLYEKTKIE PWFLTQLKEV VEAEHDLETE PHRLEDLEDW RYVKGLGLSD
510 520 530 540 550
ARVGELLGVS EAAARKQRLA AGCKPVYKTV DTCAAEFEAY TPYHYSTYEL
560 570 580 590 600
EDEVRPTSKP KVVILGSGPI RIGQGVEFDY ATVHAVWALR EAAIPPERPG
610 620 630 640 650
EAGARGYETI MVNSNPETVS TDYDTADRLY FEPLTLEDVL NLAEHEKPIG
660 670 680 690 700
VIATLGGQTP LKLAKKLAEA GVPLLGTSWE AIHKAEDRAE FNQLCAALGI
710 720 730 740 750
PQPKGAVART PEEALKLAET LGYPLMARPS YVLGGRAMQV VRNPEELRWY
760 770 780 790 800
LSGIYAALAE RPSILLDQYL ENALELDVDA LCDGKQVVVA GIMEHIERAG
810 820 830 840 850
VHSGDSATIL PPISLTPEQL ETVKAYTRKL ALAVGVRGLI NVQYAIKDGV
860 870 880 890 900
VYILEANPRA SRTVPFVSKA IGHPLAKYAA LIAVGHTLEE LGFTQDPTPS
910 920 930 940 950
FYAVKEVLIP WLKFPGVIPV LGPEMRSTGE SMGIDTDPYL AYYRAELGVG
960 970 980 990 1000
QRLPLSGKVR FIGAEQLKPD WEAAGFEVSE GDYDLLIALT PDPELRRAVE
1010 1020 1030
TGKPFITTLE GARWSLEAIL RARHSNLPVR SLQAWHGR
Length:1,038
Mass (Da):113,358
Last modified:April 20, 2010 - v1
Checksum:iA3DA9A98A1E25318
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001743 Genomic DNA. Translation: ADD27681.1.
CP005385 Genomic DNA. Translation: AGK04146.1.
RefSeqiWP_013013200.1. NC_021081.1.
YP_003506701.1. NC_013946.1.
YP_007879643.1. NC_021081.1.

Genome annotation databases

EnsemblBacteriaiADD27681; ADD27681; Mrub_0916.
AGK04146; AGK04146; K649_04220.
GeneIDi15335456.
8879003.
KEGGimrb:Mrub_0916.
mre:K649_04220.
PATRICi35357964. VBIMeiRub128672_0920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001743 Genomic DNA. Translation: ADD27681.1 .
CP005385 Genomic DNA. Translation: AGK04146.1 .
RefSeqi WP_013013200.1. NC_021081.1.
YP_003506701.1. NC_013946.1.
YP_007879643.1. NC_021081.1.

3D structure databases

ProteinModelPortali D3PQ55.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADD27681 ; ADD27681 ; Mrub_0916 .
AGK04146 ; AGK04146 ; K649_04220 .
GeneIDi 15335456.
8879003.
KEGGi mrb:Mrub_0916.
mre:K649_04220.
PATRICi 35357964. VBIMeiRub128672_0920.

Phylogenomic databases

HOGENOMi HOG000234582.
KOi K01955.
OMAi GSDRIWY.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .
BioCyci MRUB504728:GI6U-937-MONOMER.

Family and domain databases

Gene3Di 1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.20. 2 hits.
HAMAPi MF_01210_B. CPSase_L_chain_B.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005481. CarbamoylP_synth_lsu_N.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
Pfami PF00289. CPSase_L_chain. 2 hits.
PF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
[Graphical view ]
PRINTSi PR00098. CPSASE.
SMARTi SM01096. CPSase_L_D3. 1 hit.
[Graphical view ]
SUPFAMi SSF48108. SSF48108. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsi TIGR01369. CPSaseII_lrg. 1 hit.
PROSITEi PS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35948 / DSM 1279 / VKM B-1258 / 21Imported.
  2. "Non-Hybrid, Finished Microbial Genome Assemblies from Long-Read SMRT Sequencing Data."
    Klammer A., Drake J., Heiner C., Clum A., Copeland A., Huddleston J., Eichler E., Turner S.W.
    Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 1279Imported.
  3. Chin J., Alexander D.H., Marks P., Korlach J., Clum A., Copeland A.
    Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: DSM 1279Imported.

Entry informationi

Entry nameiD3PQ55_MEIRD
AccessioniPrimary (citable) accession number: D3PQ55
Entry historyi
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: October 29, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3