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D3PAI1 (D3PAI1_DEFDS) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase HAMAP-Rule MF_00168 RuleBase RU003777
EC=2.4.2.29 HAMAP-Rule MF_00168 RuleBase RU003777
Alternative name(s):
Guanine insertion enzyme HAMAP-Rule MF_00168
tRNA-guanine transglycosylase HAMAP-Rule MF_00168
Gene names
Name:tgt HAMAP-Rule MF_00168 EMBL BAI79604.1
Ordered Locus Names:DEFDS_0092 EMBL BAI79604.1
OrganismDeferribacter desulfuricans (strain DSM 14783 / JCM 11476 / NBRC 101012 / SSM1) [Complete proteome] [HAMAP] EMBL BAI79604.1
Taxonomic identifier639282 [NCBI]
Taxonomic lineageBacteria › Deferribacteres › Deferribacterales › Deferribacteraceae › Deferribacter ›

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Catalytic activity

Guanine34 in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

Guanine34 in tRNA + queuine = queuosine34 in tRNA + guanine. HAMAP-Rule MF_00168 SAAS SAAS004803

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. RuleBase RU003777

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00168 RuleBase RU003777 SAAS SAAS004803

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP-Rule MF_00168 SAAS SAAS004803

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family. HAMAP-Rule MF_00168 RuleBase RU003777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site941Nucleophile By similarity HAMAP-Rule MF_00168
Metal binding3051Zinc By similarity HAMAP-Rule MF_00168
Metal binding3071Zinc By similarity HAMAP-Rule MF_00168
Metal binding3101Zinc By similarity HAMAP-Rule MF_00168
Metal binding3361Zinc By similarity HAMAP-Rule MF_00168
Binding site951Substrate By similarity HAMAP-Rule MF_00168

Sequences

Sequence LengthMass (Da)Tools
D3PAI1 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: E2E214306C9EC3C5

FASTA37542,903
        10         20         30         40         50         60 
MVVFKFVLEK KDSESKARIG YFETIHGRVE TPIFMPVGTV GSVKTVTPHE LKEIGAQIIL 

        70         80         90        100        110        120 
GNTYHLHLRP GDDIVNHFGG LHKFINWDKP ILTDSGGFQV FSLSELKDLT EDGVTFRSHL 

       130        140        150        160        170        180 
DGRKIFLSPE KSIEIQSNLG SDIMMVLDEC VPYPCEKSYV ERSIELTAMW AKRCKEAKRN 

       190        200        210        220        230        240 
FWQALFGIVQ GGVYKDLRKI SAEQIVEIGF DGYALGGLSV GEENNIMYEI TEFTTDYLPE 

       250        260        270        280        290        300 
DRPRYLMGVG TPEDILECVE RGVDMFDCVM PTRNARNGLL FTSYGKLHIK RQDWKMSDDP 

       310        320        330        340        350        360 
IDPHCDCYTC KNFSRGYLRH LYKAGEILAL RLNTIHNLHF YISLVKEIRN AIKNGCFKKF 

       370 
KREKLEMFKI GGDNV

« Hide

References

[1]"Bacterial lifestyle in a deep-sea hydrothermal vent chimney revealed by the genome sequence of the thermophilic bacterium Deferribacter desulfuricans SSM1."
Takaki Y., Shimamura S., Nakagawa S., Fukuhara Y., Horikawa H., Ankai A., Harada T., Hosoyama A., Oguchi A., Fukui S., Fujita N., Takami H., Takai K.
DNA Res. 17:123-137(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14783 / JCM 11476 / NBRC 101012 / SSM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP011529 Genomic DNA. Translation: BAI79604.1.
RefSeqYP_003495360.1. NC_013939.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAI79604; BAI79604; DEFDS_0092.
GeneID8868501.
KEGGddf:DEFDS_0092.
PATRIC35334089. VBIDefDes107323_0094.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000223473.
KOK00773.
OMAMSPERSI.

Enzyme and pathway databases

BioCycDDES639282:GJ90-94-MONOMER.
UniPathwayUPA00392.

Family and domain databases

Gene3D3.20.20.105. 1 hit.
HAMAPMF_00168. Q_tRNA_Tgt.
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
PANTHERPTHR11962. PTHR11962. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD3PAI1_DEFDS
AccessionPrimary (citable) accession number: D3PAI1
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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