ID   D3P7S2_AZOS1            Unreviewed;       343 AA.
AC   D3P7S2;
DT   20-APR-2010, integrated into UniProtKB/TrEMBL.
DT   20-APR-2010, sequence version 1.
DT   27-MAR-2024, entry version 67.
DE   RecName: Full=Aldose 1-epimerase {ECO:0000256|PIRNR:PIRNR005096};
DE            EC=5.1.3.3 {ECO:0000256|PIRNR:PIRNR005096};
GN   Name=galM {ECO:0000313|EMBL:BAI76701.1};
GN   OrderedLocusNames=AZL_e03560 {ECO:0000313|EMBL:BAI76701.1};
OS   Azospirillum sp. (strain B510).
OG   Plasmid pAB510e {ECO:0000313|EMBL:BAI76701.1,
OG   ECO:0000313|Proteomes:UP000002040}.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=137722 {ECO:0000313|EMBL:BAI76701.1, ECO:0000313|Proteomes:UP000002040};
RN   [1] {ECO:0000313|EMBL:BAI76701.1, ECO:0000313|Proteomes:UP000002040}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B510 {ECO:0000313|EMBL:BAI76701.1,
RC   ECO:0000313|Proteomes:UP000002040};
RC   PLASMID=pAB510e {ECO:0000313|EMBL:BAI76701.1};
RX   PubMed=20047946; DOI=10.1093/dnares/dsp026;
RA   Kaneko T., Minamisawa K., Isawa T., Nakatsukasa H., Mitsui H.,
RA   Kawaharada Y., Nakamura Y., Watanabe A., Kawashima K., Ono A., Shimizu Y.,
RA   Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M., Nakazaki N.,
RA   Nakayama S., Yamada M., Tabata S., Sato S.;
RT   "Complete genomic structure of the cultivated rice endophyte Azospirillum
RT   sp. B510.";
RL   DNA Res. 17:37-50(2010).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucose = beta-D-glucose; Xref=Rhea:RHEA:10264,
CC         ChEBI:CHEBI:15903, ChEBI:CHEBI:17925; EC=5.1.3.3;
CC         Evidence={ECO:0000256|PIRNR:PIRNR005096};
CC   -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC       {ECO:0000256|ARBA:ARBA00005028, ECO:0000256|PIRNR:PIRNR005096}.
CC   -!- SIMILARITY: Belongs to the aldose epimerase family.
CC       {ECO:0000256|ARBA:ARBA00006206, ECO:0000256|PIRNR:PIRNR005096}.
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DR   EMBL; AP010951; BAI76701.1; -; Genomic_DNA.
DR   RefSeq; WP_012978491.1; NC_013859.1.
DR   AlphaFoldDB; D3P7S2; -.
DR   KEGG; azl:AZL_e03560; -.
DR   HOGENOM; CLU_031753_2_0_5; -.
DR   UniPathway; UPA00242; -.
DR   Proteomes; UP000002040; Plasmid pAB510e.
DR   GO; GO:0004034; F:aldose 1-epimerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd09019; galactose_mutarotase_like; 1.
DR   Gene3D; 2.70.98.10; -; 1.
DR   InterPro; IPR015443; Aldose_1-epimerase.
DR   InterPro; IPR008183; Aldose_1/G6P_1-epimerase.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR047215; Galactose_mutarotase-like.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   PANTHER; PTHR10091:SF50; ALDOSE 1-EPIMERASE; 1.
DR   PANTHER; PTHR10091; ALDOSE-1-EPIMERASE; 1.
DR   Pfam; PF01263; Aldose_epim; 1.
DR   PIRSF; PIRSF005096; GALM; 1.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|PIRNR:PIRNR005096};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|PIRNR:PIRNR005096};
KW   Plasmid {ECO:0000313|EMBL:BAI76701.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002040}.
FT   ACT_SITE        176
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005096-1"
FT   ACT_SITE        308
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005096-1"
FT   BINDING         78..79
FT                   /ligand="beta-D-galactose"
FT                   /ligand_id="ChEBI:CHEBI:27667"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005096-3"
FT   BINDING         176..178
FT                   /ligand="beta-D-galactose"
FT                   /ligand_id="ChEBI:CHEBI:27667"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005096-3"
FT   BINDING         243
FT                   /ligand="beta-D-galactose"
FT                   /ligand_id="ChEBI:CHEBI:27667"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR005096-2"
SQ   SEQUENCE   343 AA;  36692 MW;  42FD3A93C14CF8D1 CRC64;
     MPIESFLFDT VDGRPVEGFV LSAGGLEATV IAHGARLLRM MVPGRDGEPA DVVLGFDRLA
     DYLASDAYFG ATCGRYGNRI GGAAFTLDGV RHALAVNEPP NQLHGGPEGF DRRIWEAHVE
     EAENAVTFTL VSPDGDQGYP GTLTATTRYR LTGDGVLDIR MTATSDRPTI VNLVHHSYWN
     LGGHASGDLG DHRLAVRGGF TTPVGADLIP TGEVRPVDGT PFDLRDGVLL GEALAAVGGF
     GFDHNWCLEG PAGELRPVAV LEHPASGRRM ELATDQPGLQ IYSGGYLSER VVGKGGRPYR
     RFAGLALETQ RFPGSPNIGH FPSTRLDPGE TYSHTMRLRF SAG
//