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D3NW08 (D3NW08_AZOS1) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase HAMAP-Rule MF_00181
EC=3.4.11.1 HAMAP-Rule MF_00181
Alternative name(s):
Leucine aminopeptidase HAMAP-Rule MF_00181
Leucyl aminopeptidase HAMAP-Rule MF_00181
Gene names
Name:pepA HAMAP-Rule MF_00181 EMBL BAI72587.1
Ordered Locus Names:AZL_019490 EMBL BAI72587.1
OrganismAzospirillum sp. (strain B510) [Complete proteome] [HAMAP] EMBL BAI72587.1
Taxonomic identifier137722 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Azospirillum ›

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181 SAAS SAAS011356

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181 SAAS SAAS011356

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. HAMAP-Rule MF_00181

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family. HAMAP-Rule MF_00181

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2791 By similarity HAMAP-Rule MF_00181
Active site3531 By similarity HAMAP-Rule MF_00181
Metal binding2671Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding2721Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding2721Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding2901Manganese 2 By similarity HAMAP-Rule MF_00181
Metal binding3491Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding3511Manganese 1 By similarity HAMAP-Rule MF_00181
Metal binding3511Manganese 2 By similarity HAMAP-Rule MF_00181

Sequences

Sequence LengthMass (Da)Tools
D3NW08 [UniParc].

Last modified April 20, 2010. Version 1.
Checksum: F019935205DB721B

FASTA50252,663
        10         20         30         40         50         60 
MKFSFAKPSL PSSGVLAVTV AADRSLGPIG QELDQKTGGA LARAMAAARF NGKKDETLTL 

        70         80         90        100        110        120 
LAPAGTELDR LLLVGIGKAA DLTDLVLQGA GGAIAVALDK AADEAALLVE LPEGASLSPA 

       130        140        150        160        170        180 
AAAAEIAFGA QLRGYKFDKY RTNDRKADKK EPKPSLRKLT LLVEEDGAAK SAFKKLDTLA 

       190        200        210        220        230        240 
DAIRFTRDLV SEPANVIYPE SLAEKTRELE QFGVEVEILD QKKLRKLGMG ALLGVAQGSA 

       250        260        270        280        290        300 
NEPRVVVMRW NGNPEAEDKR PVAFIGKGVT FDSGGISIKG AAGMEDMKWD MGGSATVIGT 

       310        320        330        340        350        360 
LYALAARKAK VNAVGIVGLV ENMPSGTAQR PGDIVTSASG QTIEVINTDA EGRLVLADCL 

       370        380        390        400        410        420 
WYAQDAYKPK LMIDLATLTG AIIVALGHEH AGLFANDDDL AMNLTAAGLK VGQPLWRLPL 

       430        440        450        460        470        480 
GDAYDKEIES PAADMKNTGS GGGAGSIVGA QFLKRFVNDV PWAHIDIAGV AWAKKDGATV 

       490        500 
PKGASAFGVR LLDRFVAEHH EA

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References

[1]"Complete genomic structure of the cultivated rice endophyte Azospirillum sp. B510."
Kaneko T., Minamisawa K., Isawa T., Nakatsukasa H., Mitsui H., Kawaharada Y., Nakamura Y., Watanabe A., Kawashima K., Ono A., Shimizu Y., Takahashi C., Minami C., Fujishiro T., Kohara M., Katoh M., Nakazaki N., Nakayama S. expand/collapse author list , Yamada M., Tabata S., Sato S.
DNA Res. 17:37-50(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: B510 EMBL BAI72587.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP010946 Genomic DNA. Translation: BAI72587.1.
RefSeqYP_003449131.1. NC_013854.1.

3D structure databases

ProteinModelPortalD3NW08.
ModBaseSearch...

Protein family/group databases

MEROPSM17.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAI72587; BAI72587; AZL_019490.
GeneID8790969.
KEGGazl:AZL_019490.
PATRIC31932987. VBIAzoSp82869_2121.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000243129.
KOK01255.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERPTHR11963:SF3. PTHR11963:SF3. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameD3NW08_AZOS1
AccessionPrimary (citable) accession number: D3NW08
Entry history
Integrated into UniProtKB/TrEMBL: April 20, 2010
Last sequence update: April 20, 2010
Last modified: May 1, 2013
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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