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Protein

Acetylserotonin O-methyltransferase

Gene

Asmt

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).By similarity

Catalytic activityi

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serotonin N-acetyltransferase (Aanat), Acetylserotonin O-methyltransferase (Asmt)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei153S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei170S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei216S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei263S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei266Proton donor/acceptorBy similarity1
Binding sitei267SubstrateBy similarity1
Binding sitei317SubstrateBy similarity1

GO - Molecular functioni

  • acetylserotonin O-methyltransferase activity Source: MGI

GO - Biological processi

  • melatonin biosynthetic process Source: MGI
  • negative regulation of male gonad development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Melatonin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylserotonin O-methyltransferase (EC:2.1.1.4)
Alternative name(s):
Hydroxyindole O-methyltransferase
Gene namesi
Name:Asmt
Synonyms:Hiomt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96090. Asmt.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004147941 – 387Acetylserotonin O-methyltransferaseAdd BLAST387

Proteomic databases

PaxDbiD3KU66.
PRIDEiD3KU66.

Expressioni

Tissue specificityi

Expressed predominantly in the pineal gland. very low expression, if any, in the retina.1 Publication

Inductioni

Exhibits very slight night/day variation, if any.1 Publication

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137135.

Structurei

3D structure databases

ProteinModelPortaliD3KU66.
SMRiD3KU66.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni246 – 248S-adenosyl-L-methionine bindingPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
InParanoidiD3KU66.
KOiK00543.
TreeFamiTF314574.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR031725. ASMT_dimerisation.
IPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF16864. Dimerisation2. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3KU66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRGRSASAR QERDFRALMD LAHGFMASQV LFAGCALRVF DAAALGPVDA
60 70 80 90 100
AALARSSGLS PRGTRLLLDA CAGLGLLRRR RGAGPRGPAY TNSPLASTFL
110 120 130 140 150
VAGSPLSQRS LLLYLAGTTY LCWGHLADGV REGRSQYARA VGVDADDPFT
160 170 180 190 200
AIYRSEAERL LFMRGLQETW SLCGGRVLTA FDLSPFRVIC DLGGGSGALA
210 220 230 240 250
RMAARLYPGS EVTVFETPDV VAAARAHFPP PADEDGAEPR VRFLSGDFFR
260 270 280 290 300
SPLPPADLYV LARVLHDWAD AACVELLRRV RGALRPGGAV LLVESVLSPG
310 320 330 340 350
GAGPTRTLLL SLTMLLQARG RERTEAEYRA LTARAGFSRL RLRRPRGPYH
360 370 380
AMMAARGGGA GARSDGGGGD ATSQTGSGTG SEVGAQD
Length:387
Mass (Da):40,925
Last modified:April 20, 2010 - v1
Checksum:iDFCC2B0CDDB12680
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti78R → G in strain: C57BL/6J, 129/Sv, BALB/c, FVB/N and more; reduces protein expression. 1 Publication1
Natural varianti242R → C in strain: C57BL/6J, 129/Sv, BALB/c, FVB/N and more; reduces protein expression. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB512670 mRNA. Translation: BAI82191.1.
AB512671 mRNA. Translation: BAI82192.1.
AB512673 Genomic DNA. Translation: BAI82194.1.
AB512674 Genomic DNA. Translation: BAI82195.1.
RefSeqiNP_001186141.1. NM_001199212.1.
NP_001295417.1. NM_001308488.1.
UniGeneiMm.486144.

Genome annotation databases

GeneIDi107626.
KEGGimmu:107626.
UCSCiuc029xop.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB512670 mRNA. Translation: BAI82191.1.
AB512671 mRNA. Translation: BAI82192.1.
AB512673 Genomic DNA. Translation: BAI82194.1.
AB512674 Genomic DNA. Translation: BAI82195.1.
RefSeqiNP_001186141.1. NM_001199212.1.
NP_001295417.1. NM_001308488.1.
UniGeneiMm.486144.

3D structure databases

ProteinModelPortaliD3KU66.
SMRiD3KU66.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000137135.

Proteomic databases

PaxDbiD3KU66.
PRIDEiD3KU66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107626.
KEGGimmu:107626.
UCSCiuc029xop.1. mouse.

Organism-specific databases

CTDi438.
MGIiMGI:96090. Asmt.

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
InParanoidiD3KU66.
KOiK00543.
TreeFamiTF314574.

Enzyme and pathway databases

UniPathwayiUPA00837; UER00815.

Miscellaneous databases

PROiD3KU66.
SOURCEiSearch...

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR031725. ASMT_dimerisation.
IPR016461. O-MeTrfase_COMT.
IPR001077. O_MeTrfase_2.
IPR029063. SAM-dependent_MTases.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF16864. Dimerisation2. 1 hit.
PF00891. Methyltransf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF005739. O-mtase. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51683. SAM_OMT_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASMT_MOUSE
AccessioniPrimary (citable) accession number: D3KU66
Secondary accession number(s): D3KU65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: April 20, 2010
Last modified: November 2, 2016
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Pineal melatonin synthesis is severely compromised in most inbred strains. In many inbred strains, genetic defects in ASMT have been identified. Melatonin production may have an impact on gonadal development, testis development being significantly promoted in melatonin-deficient C57BL/6J x Mus musculus molossinus animals (PubMed:20308563).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.