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D3FWY8

- D3FWY8_BACPE

UniProt

D3FWY8 - D3FWY8_BACPE

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, BpOF4_03385
Organism
Bacillus pseudofirmus (strain OF4)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi103 – 1031Magnesium; catalytic By similarityUniRule annotation
Active sitei127 – 1271Proton acceptor By similarityUniRule annotation
Binding sitei154 – 1541Allosteric activator ADP By similarityUniRule annotation
Binding sitei162 – 1621Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei211 – 2111Allosteric activator ADP By similarityUniRule annotation
Binding sitei222 – 2221Substrate By similarityUniRule annotation
Binding sitei243 – 2431Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 732ATP By similarityUniRule annotation
Nucleotide bindingi102 – 1054ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciBPSE398511:GJI9-3106-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:BpOF4_03385Imported
OrganismiBacillus pseudofirmus (strain OF4)Imported
Taxonomic identifieri398511 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001544: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD3FWY8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni125 – 1273Substrate binding By similarityUniRule annotation
Regioni169 – 1713Substrate binding By similarityUniRule annotation
Regioni185 – 1873Allosteric activator ADP binding By similarityUniRule annotation
Regioni213 – 2153Allosteric activator ADP binding By similarityUniRule annotation
Regioni249 – 2524Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3FWY8-1 [UniParc]FASTAAdd to Basket

« Hide

MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH DLEVYGISYG YAGLIAGDIR    50
KMELGSVGDI IHRGGTMLYT ARCEEFKTLE GQKKGIEQLK KFGIEGLVVI 100
GGDGSYRGAQ KLTEHGFPTI GVPGTIDNDI PGTDYTIGFD TALNTVIDAI 150
DKIRDTATSH ERTYVIEVMG RHAGDLALWS GLADGAETIV IPEADYEMNQ 200
IISRLERGQE RGKKHSIIIV AEGAGSGMDF GKRISDATQM ETRVTVLGHI 250
QRGGSPTGAD RVLASRLGAK AVDLLLEGEA GRTVGIQNNK LVHHDINEVL 300
AQPHSVDLDM YKLSQELSI 319
Length:319
Mass (Da):34,313
Last modified:March 23, 2010 - v1
Checksum:i9B2AD89D5FEE340B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001878 Genomic DNA. Translation: ADC48743.1.
RefSeqiYP_003425635.1. NC_013791.2.

Genome annotation databases

EnsemblBacteriaiADC48743; ADC48743; BpOF4_03385.
GeneIDi8765667.
KEGGibpf:BpOF4_03385.
PATRICi31943848. VBIBacPse80461_0665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001878 Genomic DNA. Translation: ADC48743.1 .
RefSeqi YP_003425635.1. NC_013791.2.

3D structure databases

ProteinModelPortali D3FWY8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ADC48743 ; ADC48743 ; BpOF4_03385 .
GeneIDi 8765667.
KEGGi bpf:BpOF4_03385.
PATRICi 31943848. VBIBacPse80461_0665.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci BPSE398511:GJI9-3106-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4."
    Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J., Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B., Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G.
    , Xue Y., Ma Y., Hu F.Z., Krulwich T.A.
    Environ. Microbiol. 13:3289-3309(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OF4Imported.

Entry informationi

Entry nameiD3FWY8_BACPE
AccessioniPrimary (citable) accession number: D3FWY8
Entry historyi
Integrated into UniProtKB/TrEMBL: March 23, 2010
Last sequence update: March 23, 2010
Last modified: July 9, 2014
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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