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D3FWY8

- D3FWY8_BACPE

UniProt

D3FWY8 - D3FWY8_BACPE

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Bacillus pseudofirmus (strain OF4)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei127 – 1271Proton acceptorUniRule annotation
    Binding sitei154 – 1541Allosteric activator ADPUniRule annotation
    Binding sitei162 – 1621Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei211 – 2111Allosteric activator ADPUniRule annotation
    Binding sitei222 – 2221SubstrateUniRule annotation
    Binding sitei243 – 2431Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBPSE398511:GJI9-3106-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotationImported
    Ordered Locus Names:BpOF4_03385Imported
    OrganismiBacillus pseudofirmus (strain OF4)Imported
    Taxonomic identifieri398511 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001544: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD3FWY8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni125 – 1273Substrate bindingUniRule annotation
    Regioni169 – 1713Substrate bindingUniRule annotation
    Regioni185 – 1873Allosteric activator ADP bindingUniRule annotation
    Regioni213 – 2153Allosteric activator ADP bindingUniRule annotation
    Regioni249 – 2524Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3FWY8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRIGVLTSG GDSPGMNAAI RAVVRKAIYH DLEVYGISYG YAGLIAGDIR    50
    KMELGSVGDI IHRGGTMLYT ARCEEFKTLE GQKKGIEQLK KFGIEGLVVI 100
    GGDGSYRGAQ KLTEHGFPTI GVPGTIDNDI PGTDYTIGFD TALNTVIDAI 150
    DKIRDTATSH ERTYVIEVMG RHAGDLALWS GLADGAETIV IPEADYEMNQ 200
    IISRLERGQE RGKKHSIIIV AEGAGSGMDF GKRISDATQM ETRVTVLGHI 250
    QRGGSPTGAD RVLASRLGAK AVDLLLEGEA GRTVGIQNNK LVHHDINEVL 300
    AQPHSVDLDM YKLSQELSI 319
    Length:319
    Mass (Da):34,313
    Last modified:March 23, 2010 - v1
    Checksum:i9B2AD89D5FEE340B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001878 Genomic DNA. Translation: ADC48743.1.
    RefSeqiYP_003425635.1. NC_013791.2.

    Genome annotation databases

    EnsemblBacteriaiADC48743; ADC48743; BpOF4_03385.
    GeneIDi8765667.
    KEGGibpf:BpOF4_03385.
    PATRICi31943848. VBIBacPse80461_0665.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001878 Genomic DNA. Translation: ADC48743.1 .
    RefSeqi YP_003425635.1. NC_013791.2.

    3D structure databases

    ProteinModelPortali D3FWY8.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADC48743 ; ADC48743 ; BpOF4_03385 .
    GeneIDi 8765667.
    KEGGi bpf:BpOF4_03385.
    PATRICi 31943848. VBIBacPse80461_0665.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci BPSE398511:GJI9-3106-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome of alkaliphilic Bacillus pseudofirmus OF4 reveals adaptations that support the ability to grow in an external pH range from 7.5 to 11.4."
      Janto B., Ahmed A., Ito M., Liu J., Hicks D.B., Pagni S., Fackelmayer O.J., Smith T.A., Earl J., Elbourne L.D., Hassan K., Paulsen I.T., Kolsto A.B., Tourasse N.J., Ehrlich G.D., Boissy R., Ivey D.M., Li G.
      , Xue Y., Ma Y., Hu F.Z., Krulwich T.A.
      Environ. Microbiol. 13:3289-3309(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: OF4Imported.

    Entry informationi

    Entry nameiD3FWY8_BACPE
    AccessioniPrimary (citable) accession number: D3FWY8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 23, 2010
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3