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D3FHR8 (D3FHR8_MYCGH) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length846 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS006691

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01897.

Sequence similarities

Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1321O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

Sequence LengthMass (Da)Tools
D3FHR8 [UniParc].

Last modified March 23, 2010. Version 1.
Checksum: A5A16FEAAA5FB683

FASTA84694,333
        10         20         30         40         50         60 
MNPNDKNNIK ELLNKTVVKE ASITKELETS FMEYAMSVIV SRALPESRDG LKPVHRRVLY 

        70         80         90        100        110        120 
GAYTSGLTHD KPYRKSAQIV GHVMGKYHPH SDSAIYETMV RMAQPFSLRY MLIDGHGNFG 

       130        140        150        160        170        180 
SIDGDSAAAM RYTEARLSKI SAEMLRNIDK DTVDFVDNYD ASEQEPIVLP SLFPNLLANG 

       190        200        210        220        230        240 
SSGIAVGMAT NIPPHNLSEL IGGIKHLLVN ENATIEELKE FIKGPDFPTA AEILGETGIN 

       250        260        270        280        290        300 
EYFNTGRGSV SVRAKSEIEE LANNKSNIVI TQIPYMVNKA NLINKIAELV KTEQIQGIAD 

       310        320        330        340        350        360 
LRDESNREGI RIVIETKRDV IPEVLLNQLY KSTQLQTNFS VAMLALVNNQ PKVLNLKEAL 

       370        380        390        400        410        420 
QIYIDHQFDI LLRKTNFELK KAKASAHIVE GLVIATNNID DVIETIKNAK DNEDAKNTLM 

       430        440        450        460        470        480 
TKYELSDLQA KAILDMRLRS LSGLERENLQ KELAKLKELI KDLEEILQNK ERRIKIISDQ 

       490        500        510        520        530        540 
LDEIDHKFGD ERRTKICYGL NSTIDNEQLI PVETVVITRS SKGYLKRIPI SAYKVQHRGG 

       550        560        570        580        590        600 
VGVKGMNTYE DDDVESLIVC STHSDLLFFT NYGKVYRIRA HQVPLGSRIS KGIPAINLIS 

       610        620        630        640        650        660 
IEKDEKLMSL LSINDYDSGY FFFSTKKGLV KRVKASEFSR IQNNGKIAIK LTENDSLFKV 

       670        680        690        700        710        720 
IKTAGDEEIY IGVSSGLLVR FKEDVVRSMG RTAQGVIGVK FKNPNDEVIG LSSSHEGSLL 

       730        740        750        760        770        780 
LAVCEKGVGK MTDREEYRMT NRGSKGVITI KVTPKTGNII NTQLVNGNEE LLMISSTGKI 

       790        800        810        820        830        840 
VRVPLAEVSE QGRNTSGVKL ISLNEKETLQ SVAIFDVEQD DSQQVGSDNP DTELSSDDSN 


QDQDKE 

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References

[1]"Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum."
Szczepanek S.M., Tulman E.R., Gorton T.S., Liao X., Lu Z., Zinski J., Aziz F., Frasca S.Jr., Kutish G.F., Geary S.J.
Infect. Immun. 78:1760-1771(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(high / passage 156).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001872 Genomic DNA. Translation: ADC30944.1.
RefSeqYP_005880287.1. NC_017502.1.

3D structure databases

ProteinModelPortalD3FHR8.
SMRD3FHR8. Positions 40-497.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDED3FHR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC30944; ADC30944; MGAH_0612.
GeneID12395782.
KEGGmgh:MGAH_0612.
PATRIC36816743. VBIMycGal149415_0821.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000076278.
KOK02469.
OMAETVDWVP.

Enzyme and pathway databases

BioCycMGAL710128:GLH7-817-MONOMER.

Family and domain databases

Gene3D3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPMF_01897. GyrA.
InterProIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMSSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01063. gyrA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameD3FHR8_MYCGH
AccessionPrimary (citable) accession number: D3FHR8
Entry history
Integrated into UniProtKB/TrEMBL: March 23, 2010
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)