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Protein

DNA gyrase subunit A

Gene

gyrA

Organism
Mycoplasma gallisepticum (strain R(high / passage 156))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei132 – 1321O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA-dependent DNA replication Source: UniProtKB-HAMAP
  2. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, DNA-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciMGAL710128:GLH7-817-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit AUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrAUniRule annotationImported
Ordered Locus Names:MGAH_0612Imported
OrganismiMycoplasma gallisepticum (strain R(high / passage 156))Imported
Taxonomic identifieri710128 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001813 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Proteomic databases

PRIDEiD3FHR8.

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliD3FHR8.
SMRiD3FHR8. Positions 40-497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the topoisomerase GyrA/ParC subunit family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000076278.
KOiK02469.
OMAiKVYWLKV.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA.
InterProiIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01063. gyrA. 1 hit.

Sequencei

Sequence statusi: Complete.

D3FHR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPNDKNNIK ELLNKTVVKE ASITKELETS FMEYAMSVIV SRALPESRDG
60 70 80 90 100
LKPVHRRVLY GAYTSGLTHD KPYRKSAQIV GHVMGKYHPH SDSAIYETMV
110 120 130 140 150
RMAQPFSLRY MLIDGHGNFG SIDGDSAAAM RYTEARLSKI SAEMLRNIDK
160 170 180 190 200
DTVDFVDNYD ASEQEPIVLP SLFPNLLANG SSGIAVGMAT NIPPHNLSEL
210 220 230 240 250
IGGIKHLLVN ENATIEELKE FIKGPDFPTA AEILGETGIN EYFNTGRGSV
260 270 280 290 300
SVRAKSEIEE LANNKSNIVI TQIPYMVNKA NLINKIAELV KTEQIQGIAD
310 320 330 340 350
LRDESNREGI RIVIETKRDV IPEVLLNQLY KSTQLQTNFS VAMLALVNNQ
360 370 380 390 400
PKVLNLKEAL QIYIDHQFDI LLRKTNFELK KAKASAHIVE GLVIATNNID
410 420 430 440 450
DVIETIKNAK DNEDAKNTLM TKYELSDLQA KAILDMRLRS LSGLERENLQ
460 470 480 490 500
KELAKLKELI KDLEEILQNK ERRIKIISDQ LDEIDHKFGD ERRTKICYGL
510 520 530 540 550
NSTIDNEQLI PVETVVITRS SKGYLKRIPI SAYKVQHRGG VGVKGMNTYE
560 570 580 590 600
DDDVESLIVC STHSDLLFFT NYGKVYRIRA HQVPLGSRIS KGIPAINLIS
610 620 630 640 650
IEKDEKLMSL LSINDYDSGY FFFSTKKGLV KRVKASEFSR IQNNGKIAIK
660 670 680 690 700
LTENDSLFKV IKTAGDEEIY IGVSSGLLVR FKEDVVRSMG RTAQGVIGVK
710 720 730 740 750
FKNPNDEVIG LSSSHEGSLL LAVCEKGVGK MTDREEYRMT NRGSKGVITI
760 770 780 790 800
KVTPKTGNII NTQLVNGNEE LLMISSTGKI VRVPLAEVSE QGRNTSGVKL
810 820 830 840
ISLNEKETLQ SVAIFDVEQD DSQQVGSDNP DTELSSDDSN QDQDKE
Length:846
Mass (Da):94,333
Last modified:March 23, 2010 - v1
Checksum:iA5A16FEAAA5FB683
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001872 Genomic DNA. Translation: ADC30944.1.
RefSeqiYP_005880287.1. NC_017502.1.

Genome annotation databases

EnsemblBacteriaiADC30944; ADC30944; MGAH_0612.
KEGGimgh:MGAH_0612.
PATRICi36816743. VBIMycGal149415_0821.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001872 Genomic DNA. Translation: ADC30944.1.
RefSeqiYP_005880287.1. NC_017502.1.

3D structure databases

ProteinModelPortaliD3FHR8.
SMRiD3FHR8. Positions 40-497.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiD3FHR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADC30944; ADC30944; MGAH_0612.
KEGGimgh:MGAH_0612.
PATRICi36816743. VBIMycGal149415_0821.

Phylogenomic databases

HOGENOMiHOG000076278.
KOiK02469.
OMAiKVYWLKV.

Enzyme and pathway databases

BioCyciMGAL710128:GLH7-817-MONOMER.

Family and domain databases

Gene3Di3.30.1360.40. 1 hit.
3.90.199.10. 2 hits.
HAMAPiMF_01897. GyrA.
InterProiIPR024946. Arg_repress_C-like.
IPR005743. GyrA.
IPR006691. GyrA/parC_pinwhl.
IPR002205. Topo_IIA_A/C.
IPR013758. Topo_IIA_A/C_ab.
IPR013760. Topo_IIA_like_dom.
[Graphical view]
PfamiPF03989. DNA_gyraseA_C. 6 hits.
PF00521. DNA_topoisoIV. 1 hit.
[Graphical view]
SMARTiSM00434. TOP4c. 1 hit.
[Graphical view]
SUPFAMiSSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01063. gyrA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum."
    Szczepanek S.M., Tulman E.R., Gorton T.S., Liao X., Lu Z., Zinski J., Aziz F., Frasca S. Jr., Kutish G.F., Geary S.J.
    Infect. Immun. 78:1760-1771(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(high / passage 156)Imported.

Entry informationi

Entry nameiD3FHR8_MYCGH
AccessioniPrimary (citable) accession number: D3FHR8
Entry historyi
Integrated into UniProtKB/TrEMBL: March 23, 2010
Last sequence update: March 23, 2010
Last modified: April 1, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.