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D3FH24 (RNC_MYCGH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribonuclease 3

EC=3.1.26.3
Alternative name(s):
Ribonuclease III
Short name=RNase III
Gene names
Name:rnc
Ordered Locus Names:MGAH_0180
OrganismMycoplasma gallisepticum (strain R(high / passage 156)) [Complete proteome] [HAMAP]
Taxonomic identifier710128 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon By similarity. HAMAP-Rule MF_00104

Catalytic activity

Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP-Rule MF_00104

Cofactor

Mg2+ By similarity. HAMAP-Rule MF_00104

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00104

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00104.

Sequence similarities

Contains 1 DRBM (double-stranded RNA-binding) domain.

Contains 1 RNase III domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655Ribonuclease 3 HAMAP-Rule MF_00104
PRO_0000416607

Regions

Domain432 – 556125RNase III
Domain582 – 64968DRBM
Region1 – 400400Unknown HAMAP-Rule MF_00104
Region401 – 655255RNase 3 HAMAP-Rule MF_00104
Compositional bias92 – 380289Asn-rich HAMAP-Rule MF_00104

Sites

Active site4761 Potential
Active site5451 By similarity
Metal binding4721Magnesium By similarity
Metal binding5421Magnesium By similarity
Metal binding5451Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
D3FH24 [UniParc].

Last modified March 23, 2010. Version 1.
Checksum: BABBEDB7BAF8E4BE

FASTA65575,387
        10         20         30         40         50         60 
MENLEKNTKK KIKKPNNFKK NNKDKTEELK DKPTPRMFKM TPKSSKFLHQ LGVTGRKHTE 

        70         80         90        100        110        120 
KPVPLNDPDY EKKIREIQAK EAKKIRSDEV VNNNSKKQNN NKQAKKKANK NKKQKGNNNN 

       130        140        150        160        170        180 
FQNNKKPENW KQKPAANNQV KAIPNSEKST AKTAINLIIK RTFETLKQNN LIAPNLKQNP 

       190        200        210        220        230        240 
NKKDKPQQPN VAAKNNNQKE VKKPYNNFVN NQKNHNKNNA GNKGDNKQPT KPLNQSKLSK 

       250        260        270        280        290        300 
STIVELPFTP NFTSNQPKPT QKEDSKKVKA KKAENSQPQE SNKQVKKLEV KTNQQKQDQT 

       310        320        330        340        350        360 
KKKQPKENKN QQIKAVNLNN NQQKTNNNNQ KNSVDKSEND NNKKKSEANQ KQENLNPNNN 

       370        380        390        400        410        420 
NKKKEDSKNE SNNIPLINKN ISDQQIVKIS NYIKDNYPVI YADLKEKNRL GFNSNLDDDK 

       430        440        450        460        470        480 
LIVYANYEAK DLELLLKKFK VVTNNIGLYE EALTHNSYAN EMHLKYNYQR LEFLGDAIIN 

       490        500        510        520        530        540 
KIVAEYLFNH SDSSEGEMTK DRIKIIQSNT LIKAATQLEL INYIRVGEGL KIAPLSPKIL 

       550        560        570        580        590        600 
EDIFEAFIGA MYLDQGEYAV RKILNDTIIG YYQKGQLTEN TDYKSIFQEI IHSTGLNMKI 

       610        620        630        640        650 
HYERTYDRQK NLHTVSLYAG GIMYGEGKDS STHKAEIKAA KEAISKFRGL LKLEK 

« Hide

References

[1]"Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum."
Szczepanek S.M., Tulman E.R., Gorton T.S., Liao X., Lu Z., Zinski J., Aziz F., Frasca S. Jr., Kutish G.F., Geary S.J.
Infect. Immun. 78:1760-1771(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(high / passage 156).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001872 Genomic DNA. Translation: ADC30700.1.
RefSeqYP_005880043.1. NC_017502.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADC30700; ADC30700; MGAH_0180.
GeneID12395526.
KEGGmgh:MGAH_0180.
PATRIC36816211. VBIMycGal149415_0559.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03685.
OMATIVELPF.

Enzyme and pathway databases

BioCycMGAL710128:GLH7-564-MONOMER.

Family and domain databases

Gene3D1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPMF_00104. RNase_III.
InterProIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERPTHR11207. PTHR11207. 1 hit.
PfamPF00035. dsrm. 1 hit.
PF00636. Ribonuclease_3. 1 hit.
[Graphical view]
SMARTSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMSSF69065. SSF69065. 1 hit.
TIGRFAMsTIGR02191. RNaseIII. 1 hit.
PROSITEPS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNC_MYCGH
AccessionPrimary (citable) accession number: D3FH24
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2012
Last sequence update: March 23, 2010
Last modified: April 16, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families