D3FFX0 (D3FFX0_MYCGH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-2 HAMAP-Rule MF_00100 RuleBase RU000644

Gene names

Name:	infB HAMAP-Rule MF_00100 EMBL ADC30296.1
Ordered Locus Names:	MGAH_0821

Organism

Mycoplasma gallisepticum (strain R(high / passage 156)) [Complete proteome] [HAMAP]

Taxonomic identifier

710128 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma gallisepticum › Mycoplasma gallisepticum str. R

Protein attributes

Sequence length	615 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100 RuleBase RU000644
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00100 RuleBase RU000645.
Sequence similarities	Belongs to the IF-2 family. HAMAP-Rule MF_00100 RuleBase RU000644

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm HAMAP-Rule MF_00100
Ligand	GTP-binding HAMAP-Rule MF_00100 RuleBase RU000323 Nucleotide-binding
Molecular function	Initiation factor HAMAP-Rule MF_00100 RuleBase RU000644 EMBL ADC30296.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	127 – 134	8	GTP By similarity HAMAP-Rule MF_00100
	Nucleotide binding	173 – 177	5	GTP By similarity HAMAP-Rule MF_00100
	Nucleotide binding	227 – 230	4	GTP By similarity HAMAP-Rule MF_00100
	Region	121 – 269	149	G-domain By similarity HAMAP-Rule MF_00100

Sequences

	Sequence		Length	Mass (Da)
	D3FFX0 [UniParc]. Last modified March 23, 2010. Version 1. Checksum: 422C80CBB42E5974	FASTA	615	68,086
	10 20 30 40 50 60 MNKKPTRNKK PVDQRSKINI KSYLKEVQVG VKDGVFIYTD PLSIDQFAKK VNQPVAKIIK 70 80 90 100 110 120 HFFSKGINTI NLNTILSLEQ IGELCLDLGL DFKIEKSVTS ENLLDNIEFK DKKEDLVKRP 130 140 150 160 170 180 PIVTVMGHVD HGKTSLLDAI RSTNVTSNEA GGITQHIGAY QVKKNDELIT FIDTPGHEAF 190 200 210 220 230 240 TEMRARGANL TDIVVLVVAG DDGIKPQTEE AIDHAKNANV PIIVFVNKMD KSGANFDRVI 250 260 270 280 290 300 QQISKYDLSP EEYGGDTIFV QGSAIKKEGI NELLDAILTL AEINEYKANP NADPYGIVIE 310 320 330 340 350 360 SKLEPGLGPQ ATVIIKRGTL KVGDYICIGA AYGKVRIMQD ENGNNLTEAT PSRPVKISGL 370 380 390 400 410 420 DAIPQAGEKF LGLATEKEVK ELSDSYKLKQ QKQKHLSLQE SHEKRTRINT NGIKCVDLII 430 440 450 460 470 480 KSDVQGSLEA IKYAISNINI EGVTTNIIRA STGVISETDI KLAQASNSTV ISFNLGVSKQ 490 500 510 520 530 540 IRDLANSDNV QILSYEIIYK MVEDLEKIMK GELDPVYEES VIGQAVVRVL WKHSKIGTIA 550 560 570 580 590 600 GSYVTSGKVV KNALCRVLRD DVIIYKSKIA SLKSKTTFVD KVEHNKECGI VVENYNDIKE 610 DDIIEVYEIV KKRVY

References

[1]

"Comparative genomic analyses of attenuated strains of Mycoplasma gallisepticum." Szczepanek S.M., Tulman E.R., Gorton T.S., Liao X., Lu Z., Zinski J., Aziz F., Frasca S. Jr., Kutish G.F., Geary S.J. Infect. Immun. 78:1760-1771(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(high / passage 156).

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001872 Genomic DNA. Translation: ADC30296.1.
RefSeq	YP_005879639.1. NC_017502.1.
3D structure databases
ProteinModelPortal	D3FFX0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADC30296; ADC30296; MGAH_0821.
GeneID	12395896.
KEGG	mgh:MGAH_0821.
PATRIC	36815282. VBIMycGal149415_0128.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000076906.
KO	K02519.
OMA	HIGAFTV.
Family and domain databases
Gene3D	3.40.50.10050. 1 hit.
HAMAP	MF_00100_B. IF_2_B.
InterPro	IPR000795. EF_GTP-bd_dom. IPR006847. IF2_N. IPR005225. Small_GTP-bd_dom. IPR000178. TF_IF2_bacterial-like. IPR015760. TIF_IF2. IPR023115. TIF_IF2_dom3. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF41. PTHR23115:SF41. 1 hit.
Pfam	PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF11987. IF-2. 1 hit. PF04760. IF2_N. 1 hit. [Graphical view]
SUPFAM	SSF52156. TIF_IF2_dom3. 1 hit. SSF50447. Translat_factor. 2 hits.
TIGRFAMs	TIGR00487. IF-2. 1 hit. TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

D3FFX0_MYCGH

Accession

Primary (citable) accession number: D3FFX0

Entry history

Integrated into UniProtKB/TrEMBL:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)