ID   D3EDU4_GEOS4            Unreviewed;      1042 AA.
AC   D3EDU4;
DT   23-MAR-2010, integrated into UniProtKB/TrEMBL.
DT   23-MAR-2010, sequence version 1.
DT   27-MAR-2024, entry version 77.
DE   RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00013303, ECO:0000256|RuleBase:RU361154};
DE            EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU361154};
DE   AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230, ECO:0000256|RuleBase:RU361154};
GN   OrderedLocusNames=GYMC10_0767 {ECO:0000313|EMBL:ACX63067.1};
OS   Geobacillus sp. (strain Y412MC10).
OC   Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=481743 {ECO:0000313|EMBL:ACX63067.1, ECO:0000313|Proteomes:UP000002381};
RN   [1] {ECO:0000313|Proteomes:UP000002381}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y412MC10 {ECO:0000313|Proteomes:UP000002381};
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C.,
RA   Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G.,
RA   Brumm P., Mead D.;
RT   "Complete sequence of Geobacillus sp. Y412MC10.";
RL   Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:ACX63067.1, ECO:0000313|Proteomes:UP000002381}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y412MC10 {ECO:0000313|EMBL:ACX63067.1,
RC   ECO:0000313|Proteomes:UP000002381};
RX   PubMed=23408395; DOI=10.4056/sigs.2605792;
RA   Mead D.A., Lucas S., Copeland A., Lapidus A., Cheng J.F., Bruce D.C.,
RA   Goodwin L.A., Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C.S.,
RA   Tapia R., Land M., Hauser L.J., Chang Y.J., Kyrpides N.C., Ivanova N.N.,
RA   Ovchinnikova G., Woyke T., Brumm C., Hochstein R., Schoenfeld T., Brumm P.;
RT   "Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel
RT   Paenibacillus lautus strain Isolated from Obsidian Hot Spring in
RT   Yellowstone National Park.";
RL   Stand. Genomic Sci. 6:381-400(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC         in beta-D-galactosides.; EC=3.2.1.23;
CC         Evidence={ECO:0000256|ARBA:ARBA00001412,
CC         ECO:0000256|RuleBase:RU361154};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC       {ECO:0000256|ARBA:ARBA00007401, ECO:0000256|RuleBase:RU361154}.
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DR   EMBL; CP001793; ACX63067.1; -; Genomic_DNA.
DR   RefSeq; WP_012818941.1; NZ_VEAV01000007.1.
DR   AlphaFoldDB; D3EDU4; -.
DR   STRING; 481743.GYMC10_0767; -.
DR   CAZy; GH2; Glycoside Hydrolase Family 2.
DR   KEGG; gym:GYMC10_0767; -.
DR   HOGENOM; CLU_002346_0_2_9; -.
DR   Proteomes; UP000002381; Chromosome.
DR   GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR   GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR   Gene3D; 2.70.98.10; -; 1.
DR   Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR004199; B-gal_small/dom_5.
DR   InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR   InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR   InterPro; IPR008979; Galactose-bd-like_sf.
DR   InterPro; IPR014718; GH-type_carb-bd.
DR   InterPro; IPR006101; Glyco_hydro_2.
DR   InterPro; IPR023232; Glyco_hydro_2_AS.
DR   InterPro; IPR006103; Glyco_hydro_2_cat.
DR   InterPro; IPR023230; Glyco_hydro_2_CS.
DR   InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR   InterPro; IPR006104; Glyco_hydro_2_N.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR032312; LacZ_4.
DR   PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR   PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR   Pfam; PF02929; Bgal_small_N; 1.
DR   Pfam; PF00703; Glyco_hydro_2; 1.
DR   Pfam; PF02836; Glyco_hydro_2_C; 1.
DR   Pfam; PF02837; Glyco_hydro_2_N; 1.
DR   Pfam; PF16353; LacZ_4; 1.
DR   PRINTS; PR00132; GLHYDRLASE2.
DR   SMART; SM01038; Bgal_small_N; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR   SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR   SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR   PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR   PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361154};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361154}.
FT   DOMAIN          764..1035
FT                   /note="Beta galactosidase small chain/"
FT                   /evidence="ECO:0000259|SMART:SM01038"
SQ   SEQUENCE   1042 AA;  119139 MW;  58782DA248888E68 CRC64;
     MRTKFIYQAP ANGYPEWNNN PNIFQLNRHK AHAYMMNFPT ESEALSLDHA MSPWYQSLNG
     TWKFAFAKTP EERIQNFYEA DYDSESWADL PVPSHWQLQG YDYPQYTNVR YPWAESEPEL
     KAPFAPTRYN PVGSYIREFT VPDTWKEQPV YISFQGVESA FYVWVNGEMV GYSEDTFTPA
     EFDLTPYLVE GKNKLAVEVY RWCDASWLED QDFWRLSGIF REVYLYSVPA VHISDFFVRP
     VLDDEYRHAE LQIDAAVTNY YNETIDKDTL QLEALLYDQD RQLVQKQTLT IADYIIDEGS
     FTLSMNVPEP EKWSAESPYL YTVVLALKNT DGACRGAVSC RTGFRRFELK DGLMRINGQR
     ILFKGVNRHE FSPDTGRALS REDMITDIEL MKTHNINAVR TSHYPNQSVW YDLCDEYGLY
     VIDETNLETH GTWRYGQQEL EETVPGSKLE WRDNVLDRCN SMFQRDKNHP SVIIWSLGNE
     SFGGDNFIAM HDFLKEADPS RLVHYEGIFH YRASEAASDM ESTMYARPQD VEKYANSNPD
     KPYIICEYSH AMGNSCGGLH LYWELFEKYD ILQGAFIWDW IDQAILTQTA DGIPYLAYGG
     DFNETPHDGN FSGNGLIFAD RTKTPKLDEV KKCYQNVKFE SLDPAASRIR VSNRFLFTSL
     DSYLVQWDVT LNGKVSQSGT LELSLQPGEQ AEIHVPYSPV EDEGKEAILT VSLVRKESTK
     WAPAGHEEAW EQFVLTPYIA EQPETDALAN VQALEAAESD EELIIRGSAV SLRFRLGTGD
     LTSYALSGRE CLLAPARPNF WRAVTDNDLG NRLPERSGVW RTAHDRRKLL SLRWHAEGPA
     VVVSSHYRIE TNPVSSLTLE YRIYPDGTVR VHETLIPGAG LPDIPEVGML FLLDEGLDTL
     SWYGRGPHDN YWDRKTGAKI GRYEGKVADQ FVPYLRPQEC GNKTDVRFAL LTGESGGDGL
     EFESSSLMEV CALPWTPEEL EGSDHAYKLP PSDKTVLRIN YKQMGVGGDD SWGAPIHEEF
     TLPSNRTYSF GFTMGAARRP TR
//