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D3EA68

- D3EA68_GEOS4

UniProt

D3EA68 - D3EA68_GEOS4

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacillus sp. (strain Y412MC10)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (23 Mar 2010)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
    Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
    Active sitei130 – 1301Proton acceptorUniRule annotation
    Binding sitei157 – 1571Allosteric activator ADPUniRule annotation
    Binding sitei165 – 1651Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei214 – 2141Allosteric activator ADPUniRule annotation
    Binding sitei225 – 2251SubstrateUniRule annotation
    Binding sitei247 – 2471Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 762ATPUniRule annotation
    Nucleotide bindingi105 – 1084ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciPSP481743:GH8K-4661-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:GYMC10_4612Imported
    OrganismiGeobacillus sp. (strain Y412MC10)Imported
    Taxonomic identifieri481743 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
    ProteomesiUP000002381: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Structurei

    3D structure databases

    ProteinModelPortaliD3EA68.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni128 – 1303Substrate bindingUniRule annotation
    Regioni172 – 1743Substrate bindingUniRule annotation
    Regioni188 – 1903Allosteric activator ADP bindingUniRule annotation
    Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
    Regioni253 – 2564Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiMELREGH.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3EA68-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEVKKIAVL TSGGDSQGMN AALRAVVRSG LYYGLEVYGI QRGYQGLLEN    50
    DIIKMDLRSV GDIIQRGGTI LRSARCEEFK TAEGQQKGAD ILNQHGIDGL 100
    VVIGGDGSYQ GANKLSKLGI KTMGLPGTID NDISFTDYTI GFDTAVSVVV 150
    DAVNKLRDTM SSHARSSVVE VMGRHCGDIA LHAGLASGAE TILVPEVEYN 200
    LDEVATRLRE NFAKGKRHSI IIVAEGVGRG EDVVHDLKEC HASIDARVTV 250
    LGHIQRGGAP TPFDRNLASR LGDFAVRSLI DGQSDKGCGI IKGELVLTDI 300
    DKVVNTKKEF DRDLYDLALR LSQ 323
    Length:323
    Mass (Da):34,709
    Last modified:March 23, 2010 - v1
    Checksum:i61F6FC3987687F4B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001793 Genomic DNA. Translation: ACX66833.1.
    RefSeqiYP_003244640.1. NC_013406.1.

    Genome annotation databases

    EnsemblBacteriaiACX66833; ACX66833; GYMC10_4612.
    GeneIDi8518798.
    KEGGigym:GYMC10_4612.
    PATRICi32157725. VBIGeoSp56627_4572.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001793 Genomic DNA. Translation: ACX66833.1 .
    RefSeqi YP_003244640.1. NC_013406.1.

    3D structure databases

    ProteinModelPortali D3EA68.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACX66833 ; ACX66833 ; GYMC10_4612 .
    GeneIDi 8518798.
    KEGGi gym:GYMC10_4612.
    PATRICi 32157725. VBIGeoSp56627_4572.

    Phylogenomic databases

    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi MELREGH.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci PSP481743:GH8K-4661-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Geobacillus sp. Y412MC10."
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Brumm P., Mead D.
      Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Y412MC10Imported.

    Entry informationi

    Entry nameiD3EA68_GEOS4
    AccessioniPrimary (citable) accession number: D3EA68
    Entry historyi
    Integrated into UniProtKB/TrEMBL: March 23, 2010
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3