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D3EA68

- D3EA68_GEOS4

UniProt

D3EA68 - D3EA68_GEOS4

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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Geobacillus sp. (strain Y412MC10)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
Active sitei130 – 1301Proton acceptorUniRule annotation
Binding sitei157 – 1571Allosteric activator ADPUniRule annotation
Binding sitei165 – 1651Substrate; shared with dimeric partnerUniRule annotation
Binding sitei214 – 2141Allosteric activator ADPUniRule annotation
Binding sitei225 – 2251SubstrateUniRule annotation
Binding sitei247 – 2471Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762ATPUniRule annotation
Nucleotide bindingi105 – 1084ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSP481743:GH8K-4661-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:GYMC10_4612Imported
OrganismiGeobacillus sp. (strain Y412MC10)Imported
Taxonomic identifieri481743 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
ProteomesiUP000002381: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliD3EA68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni128 – 1303Substrate bindingUniRule annotation
Regioni172 – 1743Substrate bindingUniRule annotation
Regioni188 – 1903Allosteric activator ADP bindingUniRule annotation
Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
Regioni253 – 2564Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiMELREGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3EA68-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEVKKIAVL TSGGDSQGMN AALRAVVRSG LYYGLEVYGI QRGYQGLLEN
60 70 80 90 100
DIIKMDLRSV GDIIQRGGTI LRSARCEEFK TAEGQQKGAD ILNQHGIDGL
110 120 130 140 150
VVIGGDGSYQ GANKLSKLGI KTMGLPGTID NDISFTDYTI GFDTAVSVVV
160 170 180 190 200
DAVNKLRDTM SSHARSSVVE VMGRHCGDIA LHAGLASGAE TILVPEVEYN
210 220 230 240 250
LDEVATRLRE NFAKGKRHSI IIVAEGVGRG EDVVHDLKEC HASIDARVTV
260 270 280 290 300
LGHIQRGGAP TPFDRNLASR LGDFAVRSLI DGQSDKGCGI IKGELVLTDI
310 320
DKVVNTKKEF DRDLYDLALR LSQ
Length:323
Mass (Da):34,709
Last modified:March 23, 2010 - v1
Checksum:i61F6FC3987687F4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001793 Genomic DNA. Translation: ACX66833.1.
RefSeqiYP_003244640.1. NC_013406.1.

Genome annotation databases

EnsemblBacteriaiACX66833; ACX66833; GYMC10_4612.
GeneIDi8518798.
KEGGigym:GYMC10_4612.
PATRICi32157725. VBIGeoSp56627_4572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001793 Genomic DNA. Translation: ACX66833.1 .
RefSeqi YP_003244640.1. NC_013406.1.

3D structure databases

ProteinModelPortali D3EA68.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACX66833 ; ACX66833 ; GYMC10_4612 .
GeneIDi 8518798.
KEGGi gym:GYMC10_4612.
PATRICi 32157725. VBIGeoSp56627_4572.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi MELREGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci PSP481743:GH8K-4661-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Geobacillus sp. Y412MC10."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Brumm P., Mead D.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y412MC10Imported.

Entry informationi

Entry nameiD3EA68_GEOS4
AccessioniPrimary (citable) accession number: D3EA68
Entry historyi
Integrated into UniProtKB/TrEMBL: March 23, 2010
Last sequence update: March 23, 2010
Last modified: October 29, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3