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D3EA68

- D3EA68_GEOS4

UniProt

D3EA68 - D3EA68_GEOS4

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, GYMC10_4612
Organism
Geobacillus sp. (strain Y412MC10)
Status
Unreviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotationSAAS annotations

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotations

Cofactori

Magnesium By similarity.UniRule annotationSAAS annotations

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141ATP; via amide nitrogen By similarityUniRule annotation
Metal bindingi106 – 1061Magnesium; catalytic By similarityUniRule annotation
Active sitei130 – 1301Proton acceptor By similarityUniRule annotation
Binding sitei157 – 1571Allosteric activator ADP By similarityUniRule annotation
Binding sitei165 – 1651Substrate; shared with dimeric partner By similarityUniRule annotation
Binding sitei214 – 2141Allosteric activator ADP By similarityUniRule annotation
Binding sitei225 – 2251Substrate By similarityUniRule annotation
Binding sitei247 – 2471Substrate; shared with dimeric partner By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762ATP By similarityUniRule annotation
Nucleotide bindingi105 – 1084ATP By similarityUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationSAAS annotationsImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, MagnesiumUniRule annotationSAAS annotations, Metal-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciPSP481743:GH8K-4661-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkAUniRule annotation
Ordered Locus Names:GYMC10_4612Imported
OrganismiGeobacillus sp. (strain Y412MC10)Imported
Taxonomic identifieri481743 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPaenibacillaceaePaenibacillus
ProteomesiUP000002381: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotationSAAS annotations

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotations

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotationSAAS annotations

Structurei

3D structure databases

ProteinModelPortaliD3EA68.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partner By similarityUniRule annotation
Regioni128 – 1303Substrate binding By similarityUniRule annotation
Regioni172 – 1743Substrate binding By similarityUniRule annotation
Regioni188 – 1903Allosteric activator ADP binding By similarityUniRule annotation
Regioni216 – 2183Allosteric activator ADP binding By similarityUniRule annotation
Regioni253 – 2564Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000248870.
KOiK00850.
OMAiMELREGH.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3EA68-1 [UniParc]FASTAAdd to Basket

« Hide

MAEVKKIAVL TSGGDSQGMN AALRAVVRSG LYYGLEVYGI QRGYQGLLEN    50
DIIKMDLRSV GDIIQRGGTI LRSARCEEFK TAEGQQKGAD ILNQHGIDGL 100
VVIGGDGSYQ GANKLSKLGI KTMGLPGTID NDISFTDYTI GFDTAVSVVV 150
DAVNKLRDTM SSHARSSVVE VMGRHCGDIA LHAGLASGAE TILVPEVEYN 200
LDEVATRLRE NFAKGKRHSI IIVAEGVGRG EDVVHDLKEC HASIDARVTV 250
LGHIQRGGAP TPFDRNLASR LGDFAVRSLI DGQSDKGCGI IKGELVLTDI 300
DKVVNTKKEF DRDLYDLALR LSQ 323
Length:323
Mass (Da):34,709
Last modified:March 23, 2010 - v1
Checksum:i61F6FC3987687F4B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001793 Genomic DNA. Translation: ACX66833.1.
RefSeqiYP_003244640.1. NC_013406.1.

Genome annotation databases

EnsemblBacteriaiACX66833; ACX66833; GYMC10_4612.
GeneIDi8518798.
KEGGigym:GYMC10_4612.
PATRICi32157725. VBIGeoSp56627_4572.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001793 Genomic DNA. Translation: ACX66833.1 .
RefSeqi YP_003244640.1. NC_013406.1.

3D structure databases

ProteinModelPortali D3EA68.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACX66833 ; ACX66833 ; GYMC10_4612 .
GeneIDi 8518798.
KEGGi gym:GYMC10_4612.
PATRICi 32157725. VBIGeoSp56627_4572.

Phylogenomic databases

HOGENOMi HOG000248870.
KOi K00850.
OMAi MELREGH.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci PSP481743:GH8K-4661-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Geobacillus sp. Y412MC10."
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Brumm P., Mead D.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y412MC10.

Entry informationi

Entry nameiD3EA68_GEOS4
AccessioniPrimary (citable) accession number: D3EA68
Entry historyi
Integrated into UniProtKB/TrEMBL: March 23, 2010
Last sequence update: March 23, 2010
Last modified: July 9, 2014
This is version 31 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome

External Data

Dasty 3

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