D3E4X7 (D3E4X7_GEOS4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Leucine--tRNA ligase HAMAP-Rule MF_00049
EC=6.1.1.4 HAMAP-Rule MF_00049

Alternative name(s):
Leucyl-tRNA synthetase HAMAP-Rule MF_00049

Gene names

Name:	leuS HAMAP-Rule MF_00049
Ordered Locus Names:	GYMC10_1721

Organism

Geobacillus sp. (strain Y412MC10) [Complete proteome] [HAMAP] EMBL ACX64004.1

Taxonomic identifier

481743 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00049.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP-Rule MF_00049 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP-Rule MF_00049 RuleBase RU003489
Cellular component	Cytoplasm HAMAP-Rule MF_00049
Ligand	ATP-binding HAMAP-Rule MF_00049 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP-Rule MF_00049 RuleBase RU003489 EMBL ACX64004.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	leucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro leucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	48 – 59	12	"HIGH" region By similarity HAMAP-Rule MF_00049
	Motif	584 – 588	5	"KMSKS" region By similarity HAMAP-Rule MF_00049
Sites
	Binding site	587	1	ATP By similarity HAMAP-Rule MF_00049

Sequences

	Sequence		Length	Mass (Da)
	D3E4X7 [UniParc]. Last modified March 23, 2010. Version 1. Checksum: 3E9927244BF3668B	FASTA	813	92,710
	10 20 30 40 50 60 MTDNQPTGHG YQPQAIEPKW QQYWDENKTF KTEETSSKPK FYALDMFPYP SGAGLHVGHP 70 80 90 100 110 120 EGYTATDIVS RYKRMRGYNV LHPMGWDAFG LPAEQYAMDT GKHPREFTVQ NIDTFRRQIK 130 140 150 160 170 180 SLGFSYDWDR EISTTDPEYY KWTQWIFIQL YKRGLAYVAE VPVNWCEALG TVLANEEVID 190 200 210 220 230 240 GKSERGGHPV VRKPMRQWIL RITEYADRLL EDLDELDWSE SLKDMQRNWI GKSTGAEVRF 250 260 270 280 290 300 DIEGYDGHLE VFTTRPDTLF GASYCVLAPE HDLVDKIATA DQREAVKAYQ DQAARKSDLE 310 320 330 340 350 360 RTDLAKDKTG VFTGAYAVNP VNGAKVPIWI ADYVLAGYGT GAIMAVPGHD SRDWEFAKQF 370 380 390 400 410 420 GLPIIEVVQG GNVEEEAYTG TGAHVNSGFL NGLDNEAAIQ AMNEWLEKEG KGKKKVTYRL 430 440 450 460 470 480 RDWLFSRQRY WGEPIPILHL EDGTMKTVPE DQLPLLLPDV DQIKPSGTGE SPLANVTEWV 490 500 510 520 530 540 ETVDPETGMK ARRETNTMPQ WAGSCWYYLR YIDPDNHDEL CSKEKQKEWL PVDLYIGGVE 550 560 570 580 590 600 HAVLHLLYAR FWHKVLYDLG VVDTKEPFQK LVNQGMILGN NNEKMSKSRG NVINPDDIVN 610 620 630 640 650 660 EYGADTLRVY EMFMGPLEAT KPWNTNGVEG THRFLSRVWR LFIGDDGQLN PKIVDGEGTD 670 680 690 700 710 720 EFKRTLHKTI KKVTDDFEHL RFNTAISQLM IFINEGYKSE VLPRKAMENF VQLLSPLAPH 730 740 750 760 770 780 LAEELWSRLG YTESVTYVPW PEFDEAWTVE AEVEIVVQVN GKIVERAKIA KDMDQEAMQQ 790 800 810 HSLSLPNVKQ ALEGKSIRKI IAVPGKLVNI VAG

References

[1]

"Complete sequence of Geobacillus sp. Y412MC10." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Saunders E., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Brumm P., Mead D. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Y412MC10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001793 Genomic DNA. Translation: ACX64004.1.
RefSeq	YP_003241811.1. NC_013406.1.
3D structure databases
ProteinModelPortal	D3E4X7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACX64004; ACX64004; GYMC10_1721.
GeneID	8515900.
KEGG	gym:GYMC10_1721.
PATRIC	32152047. VBIGeoSp56627_1739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000200748.
KO	K01869.
OMA	WIFLQIF.
Enzyme and pathway databases
BioCyc	PSP481743:GH8K-1763-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_00049_B. Leu_tRNA_synth_B.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002302. Leu-tRNA-ligase_bac/mito. IPR025709. Leu_tRNA-synth_edit. IPR015413. Methionyl/Leucyl_tRNA_Synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF13603. tRNA-synt_1_2. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR00985. TRNASYNTHLEU.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00396. leuS_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

D3E4X7_GEOS4

Accession

Primary (citable) accession number: D3E4X7

Entry history

Integrated into UniProtKB/TrEMBL:	March 23, 2010
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)