Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

D3BT31

- TRM5_POLPA

UniProt

D3BT31 - TRM5_POLPA

Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Polysphondylium pallidum (Cellular slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 15 (01 Oct 2014)
      Sequence version 1 (23 Mar 2010)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei203 – 2031S-adenosyl-L-methionineUniRule annotation
    Binding sitei292 – 2921S-adenosyl-L-methionineUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
    Alternative name(s):
    M1G-methyltransferaseUniRule annotation
    tRNA [GM37] methyltransferaseUniRule annotation
    tRNA methyltransferase 5 homologUniRule annotation
    Gene namesi
    Name:trmt5
    OrganismiPolysphondylium pallidum (Cellular slime mold)
    Taxonomic identifieri13642 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaPolysphondylium
    ProteomesiUP000001396: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
    Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426tRNA (guanine(37)-N1)-methyltransferasePRO_0000414134Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni242 – 2432S-adenosyl-L-methionine bindingUniRule annotation
    Regioni269 – 2702S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03152. TRM5.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view]
    PfamiPF02475. Met_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    D3BT31-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD    50
    SDSKDKKLVL LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ 100
    LNYNNFSYEE VMKELIPTGL PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ 150
    VILDKKGPQI RTVLNKVGKI DTVFRTFNFE LLAGDNDLLA QVVYWNSRLQ 200
    FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA NDLNPNSVKY 250
    MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF 300
    LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII 350
    KHPLPADTTV YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS 400
    SNNNNNNKID YNEAVSSGGE GKKIKH 426
    Length:426
    Mass (Da):48,106
    Last modified:March 23, 2010 - v1
    Checksum:i4C79BA888DAF1800
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ADBJ01000056 Genomic DNA. Translation: EFA75248.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    ADBJ01000056 Genomic DNA. Translation: EFA75248.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03152. TRM5.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025792. tRNA_Gua_MeTrfase_euk.
    IPR003402. tRNA_Trfase_Trm5/Tyw2.
    [Graphical view ]
    Pfami PF02475. Met_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Living fossils from the dawn of multicellularity."
      Gloeckner G., Schaap P., Noegel A.A., Felder M., Eichinger L., Heidel A.J., Platzer M.
      Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PN500.

    Entry informationi

    Entry nameiTRM5_POLPA
    AccessioniPrimary (citable) accession number: D3BT31
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 14, 2011
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 15 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3