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D3BT31 (TRM5_POLPA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 13. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine(37)-N1)-methyltransferase

EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog
Gene names
Name:trmt5
OrganismPolysphondylium pallidum (Cellular slime mold) [Reference proteome]
Taxonomic identifier13642 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaPolysphondylium

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding By similarity. HAMAP-Rule MF_03152

Catalytic activity

S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_03152

Subunit structure

Monomer By similarity. HAMAP-Rule MF_03152

Subcellular location

Mitochondrion matrix By similarity. Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly in the mitochondria and in the nucleus By similarity. HAMAP-Rule MF_03152

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
Mitochondrion
Nucleus
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine(37)-N(1))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 426426tRNA (guanine(37)-N1)-methyltransferase HAMAP-Rule MF_03152
PRO_0000414134

Regions

Region242 – 2432S-adenosyl-L-methionine binding By similarity
Region269 – 2702S-adenosyl-L-methionine binding By similarity

Sites

Binding site2031S-adenosyl-L-methionine By similarity
Binding site2921S-adenosyl-L-methionine By similarity

Sequences

Sequence LengthMass (Da)Tools
D3BT31 [UniParc].

Last modified March 23, 2010. Version 1.
Checksum: 4C79BA888DAF1800

FASTA42648,106
        10         20         30         40         50         60 
MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD SDSKDKKLVL 

        70         80         90        100        110        120 
LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ LNYNNFSYEE VMKELIPTGL 

       130        140        150        160        170        180 
PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ VILDKKGPQI RTVLNKVGKI DTVFRTFNFE 

       190        200        210        220        230        240 
LLAGDNDLLA QVVYWNSRLQ FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA 

       250        260        270        280        290        300 
NDLNPNSVKY MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF 

       310        320        330        340        350        360 
LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII KHPLPADTTV 

       370        380        390        400        410        420 
YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS SNNNNNNKID YNEAVSSGGE 


GKKIKH 

« Hide

References

[1]"Living fossils from the dawn of multicellularity."
Gloeckner G., Schaap P., Noegel A.A., Felder M., Eichinger L., Heidel A.J., Platzer M.
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PN500.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
ADBJ01000056 Genomic DNA. Translation: EFA75248.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_03152. TRM5.
InterProIPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamPF02475. Met_10. 1 hit.
[Graphical view]
PROSITEPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRM5_POLPA
AccessionPrimary (citable) accession number: D3BT31
Entry history
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: March 23, 2010
Last modified: February 19, 2014
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families