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D3BT31

- TRM5_POLPA

UniProt

D3BT31 - TRM5_POLPA

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Protein
tRNA (guanine(37)-N1)-methyltransferase
Gene
trmt5
Organism
Polysphondylium pallidum (Cellular slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031S-adenosyl-L-methionine By similarity
Binding sitei292 – 2921S-adenosyl-L-methionine By similarity

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferase (EC:2.1.1.228)
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog
Gene namesi
Name:trmt5
OrganismiPolysphondylium pallidum (Cellular slime mold)
Taxonomic identifieri13642 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaPolysphondylium
ProteomesiUP000001396: Unassembled WGS sequence

Subcellular locationi

Mitochondrion matrix By similarity. Nucleus By similarity. Cytoplasm By similarity
Note: Predominantly in the mitochondria and in the nucleus By similarity.UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation
PRO_0000414134Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 2432S-adenosyl-L-methionine binding By similarity
Regioni269 – 2702S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3BT31-1 [UniParc]FASTAAdd to Basket

« Hide

MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD    50
SDSKDKKLVL LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ 100
LNYNNFSYEE VMKELIPTGL PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ 150
VILDKKGPQI RTVLNKVGKI DTVFRTFNFE LLAGDNDLLA QVVYWNSRLQ 200
FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA NDLNPNSVKY 250
MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF 300
LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII 350
KHPLPADTTV YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS 400
SNNNNNNKID YNEAVSSGGE GKKIKH 426
Length:426
Mass (Da):48,106
Last modified:March 23, 2010 - v1
Checksum:i4C79BA888DAF1800
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ADBJ01000056 Genomic DNA. Translation: EFA75248.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
ADBJ01000056 Genomic DNA. Translation: EFA75248.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_03152. TRM5.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR025792. tRNA_Gua_MeTrfase_euk.
IPR003402. tRNA_Trfase_Trm5/Tyw2.
[Graphical view ]
Pfami PF02475. Met_10. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Living fossils from the dawn of multicellularity."
    Gloeckner G., Schaap P., Noegel A.A., Felder M., Eichinger L., Heidel A.J., Platzer M.
    Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PN500.

Entry informationi

Entry nameiTRM5_POLPA
AccessioniPrimary (citable) accession number: D3BT31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: March 23, 2010
Last modified: June 11, 2014
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3