D3BT31 (TRM5_POLPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine(37)-N1)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog

Gene names

Name:

trmt5

Organism

Polysphondylium pallidum (Cellular slime mold) [Reference proteome]

Taxonomic identifier

13642 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Polysphondylium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding By similarity. HAMAP-Rule MF_03152
Catalytic activity	S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine37 in tRNA. HAMAP-Rule MF_03152
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion matrix By similarity. Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly in the mitochondria and in the nucleus By similarity.
Sequence similarities	Belongs to the TRM5 / TYW2 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Mitochondrion Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine(37)-N(1))-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 426	426	tRNA (guanine(37)-N1)-methyltransferase HAMAP-Rule MF_03152		PRO_0000414134
Regions
	Region	242 – 243	2	S-adenosyl-L-methionine binding By similarity
	Region	269 – 270	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	203	1	S-adenosyl-L-methionine By similarity
	Binding site	292	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	D3BT31 [UniParc]. Last modified March 23, 2010. Version 1. Checksum: 4C79BA888DAF1800	FASTA	426	48,106
	10 20 30 40 50 60 MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD SDSKDKKLVL 70 80 90 100 110 120 LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ LNYNNFSYEE VMKELIPTGL 130 140 150 160 170 180 PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ VILDKKGPQI RTVLNKVGKI DTVFRTFNFE 190 200 210 220 230 240 LLAGDNDLLA QVVYWNSRLQ FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA 250 260 270 280 290 300 NDLNPNSVKY MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF 310 320 330 340 350 360 LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII KHPLPADTTV 370 380 390 400 410 420 YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS SNNNNNNKID YNEAVSSGGE GKKIKH

References

[1]	"Living fossils from the dawn of multicellularity." Gloeckner G., Schaap P., Noegel A.A., Felder M., Eichinger L., Heidel A.J., Platzer M. Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PN500.

Cross-references

Sequence databases
EMBL GenBank DDBJ	ADBJ01000056 Genomic DNA. Translation: EFA75248.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_03152. TRM5.
InterPro	IPR003402. tRNA_Trfase_Trm5/Tyw2. [Graphical view]
Pfam	PF02475. Met_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM5_POLPA

Accession

Primary (citable) accession number: D3BT31

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 14, 2011
Last sequence update:	March 23, 2010
Last modified:	February 6, 2013
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)