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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Polysphondylium pallidum (Cellular slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031S-adenosyl-L-methionineUniRule annotation
Binding sitei292 – 2921S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine(37)-N(1))-methyltransferase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:trmt5
OrganismiPolysphondylium pallidum (Cellular slime mold)
Taxonomic identifieri13642 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaPolysphondylium
ProteomesiUP000001396 Componenti: Unassembled WGS sequence

Subcellular locationi

Mitochondrion matrix UniRule annotation. Nucleus UniRule annotation. Cytoplasm UniRule annotation
Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 426426tRNA (guanine(37)-N1)-methyltransferasePRO_0000414134Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni242 – 2432S-adenosyl-L-methionine bindingUniRule annotation
Regioni269 – 2702S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Phylogenomic databases

InParanoidiD3BT31.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

D3BT31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD
60 70 80 90 100
SDSKDKKLVL LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ
110 120 130 140 150
LNYNNFSYEE VMKELIPTGL PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ
160 170 180 190 200
VILDKKGPQI RTVLNKVGKI DTVFRTFNFE LLAGDNDLLA QVVYWNSRLQ
210 220 230 240 250
FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA NDLNPNSVKY
260 270 280 290 300
MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF
310 320 330 340 350
LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII
360 370 380 390 400
KHPLPADTTV YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS
410 420
SNNNNNNKID YNEAVSSGGE GKKIKH
Length:426
Mass (Da):48,106
Last modified:March 22, 2010 - v1
Checksum:i4C79BA888DAF1800
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADBJ01000056 Genomic DNA. Translation: EFA75248.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADBJ01000056 Genomic DNA. Translation: EFA75248.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiD3BT31.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03152. TRM5.
InterProiIPR030382. MeTrfase_TRM5/TYW2.
IPR029063. SAM-dependent_MTases.
IPR025792. tRNA_Gua_MeTrfase_euk.
[Graphical view]
PfamiPF02475. Met_10. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51684. SAM_MT_TRM5_TYW2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Living fossils from the dawn of multicellularity."
    Gloeckner G., Schaap P., Noegel A.A., Felder M., Eichinger L., Heidel A.J., Platzer M.
    Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PN500.

Entry informationi

Entry nameiTRM5_POLPA
AccessioniPrimary (citable) accession number: D3BT31
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2011
Last sequence update: March 22, 2010
Last modified: January 6, 2015
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.