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Protein

tRNA (guanine(37)-N1)-methyltransferase

Gene

trmt5

Organism
Polysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) (Heterostelium pallidum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine(37) in tRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei203S-adenosyl-L-methionineUniRule annotation1
Binding sitei292S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(37)-N1)-methyltransferaseUniRule annotation (EC:2.1.1.228UniRule annotation)
Alternative name(s):
M1G-methyltransferaseUniRule annotation
tRNA [GM37] methyltransferaseUniRule annotation
tRNA methyltransferase 5 homologUniRule annotation
Gene namesi
Name:trmt5
ORF Names:PPL_11323
OrganismiPolysphondylium pallidum (strain ATCC 26659 / Pp 5 / PN500) (Heterostelium pallidum)
Taxonomic identifieri670386 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsAcytostelialesAcytosteliaceaeHeterostelium
Proteomesi
  • UP000001396 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Mitochondrion matrix UniRule annotation
  • Nucleus UniRule annotation
  • Cytoplasm UniRule annotation
  • Note: Predominantly in the mitochondria and in the nucleus.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004141341 – 426tRNA (guanine(37)-N1)-methyltransferaseAdd BLAST426

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni242 – 243S-adenosyl-L-methionine bindingUniRule annotation2
Regioni269 – 270S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Phylogenomic databases

InParanoidiD3BT31
OMAiKHLIGQV

Family and domain databases

HAMAPiMF_03152 TRM5, 1 hit
InterProiView protein in InterPro
IPR030382 MeTrfase_TRM5/TYW2
IPR029063 SAM-dependent_MTases
IPR025792 tRNA_Gua_MeTrfase_euk
PfamiView protein in Pfam
PF02475 Met_10, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51684 SAM_MT_TRM5_TYW2, 1 hit

Sequencei

Sequence statusi: Complete.

D3BT31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLLLNKEI FSKSISIVGL KVNKTLLKTL AQEFKGYLLI RDRVKHIVDD
60 70 80 90 100
SDSKDKKLVL LSESIENGNV GAYGVNKQLP DNLQSFLKTH SIEVVKHQVQ
110 120 130 140 150
LNYNNFSYEE VMKELIPTGL PIPHAFEKIG HIAHLNLKEE LLPYKNMIGQ
160 170 180 190 200
VILDKKGPQI RTVLNKVGKI DTVFRTFNFE LLAGDNDLLA QVVYWNSRLQ
210 220 230 240 250
FEHSNLIQTF KSHDIVVDMF AGVGPFAVPA SKLVKCKVYA NDLNPNSVKY
260 270 280 290 300
MRENATRNKA STIEISNLDA RDFVRELVSR DPPVAFTQAI MNLPSTSIEF
310 320 330 340 350
LDVFREIFLN PEKAPPIPAP TIHCYTFTPV SETAGGDLKE LTIKNVEAII
360 370 380 390 400
KHPLPADTTV YEVRDVSPNK RMMRISFKMP TLKKRKDTEN NDDQENNNNS
410 420
SNNNNNNKID YNEAVSSGGE GKKIKH
Length:426
Mass (Da):48,106
Last modified:March 23, 2010 - v1
Checksum:i4C79BA888DAF1800
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ADBJ01000056 Genomic DNA Translation: EFA75248.1

Genome annotation databases

EnsemblProtistsiEFA75248; EFA75248; PPL_11323

Similar proteinsi

Entry informationi

Entry nameiTRM5_POLPP
AccessioniPrimary (citable) accession number: D3BT31
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 14, 2011
Last sequence update: March 23, 2010
Last modified: March 28, 2018
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health