D2YW48 (GST_COCIM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione S-transferase
EC=2.5.1.18

Gene names

ORF Names:

CIMG_01314

Organism

Coccidioides immitis (strain RS) (Valley fever fungus) [Reference proteome]

Taxonomic identifier

246410 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Onygenales › mitosporic Onygenales › Coccidioides › Coccidioides immitis

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable glutathione S-transferase.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer Probable.
Sequence similarities	Belongs to the GST superfamily. Zeta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 231

231

Probable glutathione S-transferase

PRO_0000404254

Regions

Domain

4 – 96

93

GST N-terminal

Domain

105 – 227

123

GST C-terminal

Region

80 – 81

2

Glutathione binding

Region

128 – 130

3

Glutathione binding

Sites

Binding site

14

1

Glutathione

Binding site

43

1

Glutathione

Binding site

57

1

Glutathione; via amide nitrogen and carbonyl oxygen

Binding site

124

1

Glutathione

Secondary structure

1

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231

Helix Strand Turn

Details...

Beta strand

5 – 10

6

Helix

15 – 26

12

Beta strand

32 – 35

4

Helix

38 – 40

3

Helix

42 – 44

3

Helix

46 – 51

6

Beta strand

53 – 55

3

Beta strand

59 – 65

7

Beta strand

68 – 70

3

Beta strand

74 – 78

5

Helix

81 – 91

11

Helix

106 – 122

17

Helix

125 – 127

3

Helix

129 – 137

9

Helix

142 – 164

23

Beta strand

165 – 171

7

Helix

176 – 190

15

Helix

195 – 197

3

Helix

199 – 209

11

Helix

212 – 216

5

Helix

219 – 221

3

Helix

227 – 229

3

Sequences

	Sequence		Length	Mass (Da)
	D2YW48 [UniParc]. Last modified February 8, 2011. Version 2. Checksum: 6D6D57E813D63A07	FASTA	231	25,585
	10 20 30 40 50 60 MTTPNFELYG YFRSSCSGRL RIAFHLKSIP YTRHPVNLLK GEQHSDTYKS LNPTNTVPLL 70 80 90 100 110 120 VVSNINNTVS PSSASFSIGQ SLAALEYLEE ALPTNARPLL PPISNPVARA HVRTICNIIA 130 140 150 160 170 180 CDVQPVTNLK IQKKVKALDG DPTVWSRDLA TQGFGAVEKL LELSAGRFCV GDEITLADVC 190 200 210 220 230 LVPAVWAAER VGMDLARFPI TKRVFEEMLK EEAVQKAHWQ KQEDTPEDLR A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative genomic analyses of the human fungal pathogens Coccidioides and their relatives." Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R., Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y., McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M., Barker B.M., Galgiani J.N. , Orbach M.J., Kirkland T.N., Cole G.T., Henn M.R., Birren B.W., Taylor J.W. Genome Res. 19:1722-1731(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RS.
[2]	"Population genomic sequencing of Coccidioides fungi reveals recent hybridization and transposon control." Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J., Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D., Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A., FitzGerald M. , Lui A., Macdonald J.P., Priest M., Orbach M.J., Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R., Taylor J.W., Rounsley S.D. Genome Res. 20:938-946(2010) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: RS.
[3]	"Crystal structure of putative glutathione transferase from Coccidioides immitis bound to glutathione." Seattle structural genomics center for infectious disease (SSGCID) Submitted (JUN-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE. Strain: RS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

GG704911 Genomic DNA. Translation: EAS36059.2.

RefSeq

XP_001247543.1. XM_001247542.1.
XP_001247642.1. XM_001247641.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3LG6	X-ray	2.20	A/B/C/D	1-231	[»]
3N5O	X-ray	1.85	A/B	1-231	[»]

ProteinModelPortal

D2YW48.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

4567107.
4567214.

KEGG

cim:CIMG_01314.
cim:CIMG_01413.

Phylogenomic databases

KO

K01783.
K01800.

Family and domain databases

Gene3D

1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.

InterPro

IPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Pfam

PF00043. GST_C. 1 hit.
[Graphical view]

SUPFAM

SSF47616. GST_C_like. 1 hit.
SSF52833. Thiordxn-like_fd. 1 hit.

TIGRFAMs

TIGR01262. maiA. 1 hit.

PROSITE

PS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

D2YW48.

Entry information

Entry name

GST_COCIM

Accession

Primary (citable) accession number: D2YW48
Secondary accession number(s): J3KJ57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	February 8, 2011
Last modified:	May 1, 2013
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program