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Protein

Mu-theraphotoxin-Hhn1b 1

Gene
N/A
Organism
Haplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neurotoxin. Selectively blocks neuronal tetrodotoxin-sensitive voltage-gated sodium channels (Nav) with an IC50 of 44.6 nM. Does not affect tetrodotoxin-resistant voltage-gated sodium channels or calcium channels.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei76Interacts with channel1
Sitei78Interacts with channel1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-theraphotoxin-Hhn1b 1
Short name:
Mu-TRTX-Hhn1b
Alternative name(s):
Hainantoxin-4
Hainantoxin-IV
Short name:
HnTx-IV
Peptide F8-18.88
OrganismiHaplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Taxonomic identifieri209901 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000340. mu-theraphotoxin-Hhn1b.

Subcellular locationi

  • Secreted 2 Publications

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.2 +-0.07 mg/kg by intraperitoneal injection into mice.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61S → A: No important change in activity. 2 Publications1
Mutagenesisi75R → A: No important change in activity. 1 Publication1
Mutagenesisi76K → A: Important reduction in activity, without change of toxin conformation. 1 Publication1
Mutagenesisi78R → A: Important reduction in activity, without change of toxin conformation. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000040056622 – 493 PublicationsAdd BLAST28
PeptideiPRO_000040056750 – 84Mu-theraphotoxin-Hhn1b 13 PublicationsAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi51 ↔ 661 Publication
Disulfide bondi58 ↔ 731 Publication
Disulfide bondi65 ↔ 801 Publication
Modified residuei84Isoleucine amide2 Publications1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.2 Publications

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

186
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni60 – 62Combined sources3
Turni67 – 70Combined sources4
Beta strandi71 – 73Combined sources3
Beta strandi75 – 77Combined sources3
Beta strandi79 – 82Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NIYNMR-A50-84[»]
1RYGNMR-A50-84[»]
1RYVNMR-A50-84[»]
SMRiD2Y232.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD2Y232.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Htx-4 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2Y232-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKASMFLALA GLALLFVVCY ASESEEKEFS NELLSSVLAV DDNSKGEERE
60 70 80
CLGFGKGCNP SNDQCCKSSN LVCSRKHRWC KYEIGK
Length:86
Mass (Da):9,505
Last modified:March 2, 2010 - v1
Checksum:i88497767C83CB6DD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61 – 64SNDQ → DQSN AA sequence (PubMed:14512091).Curated4

Mass spectrometryi

Molecular mass is 3988.58 Da from positions 50 - 84. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU292909 mRNA. Translation: ADB56725.1.

Similar proteinsi

Entry informationi

Entry nameiH4A01_HAPHA
AccessioniPrimary (citable) accession number: D2Y232
Secondary accession number(s): P83471
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 2, 2010
Last modified: November 2, 2016
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families