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Protein

Hainantoxin-III 1

Gene
N/A
Organism
Haplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selective antagonist of neuronal tetrodotoxin (TTX)-sensitive voltage-gated sodium channels (IC50=1270 nM on Nav1.1/SCN1A, 270 nM on Nav1.2/SCN2A, 491 nM on Nav1.3/SCN3A and 232 nM on Nav1.7/SCN9A). This toxin suppress Nav1.7 current amplitude without significantly altering the activation, inactivation, and repriming kinetics. Short extreme depolarizations partially activate the toxin-bound channel, indicating voltage-dependent inhibition of this toxin. This toxin increases the deactivation of the Nav1.7 current after extreme depolarizations. The toxin-Nav1.7 complex is gradually dissociated upon prolonged strong depolarizations in a voltage-dependent manner, and the unbound toxin rebinds to Nav1.7 after a long repolarization. Moreover, analysis of chimeric channels showed that the DIIS3-S4 linker is critical for toxin binding to Nav1.7. These data are consistent with this toxin interacting with Nav1.7 site 4 and trapping the domain II voltage sensor in the closed state.1 Publication

Miscellaneous

Has no activity on Nav1.4, Nav1.5, Nav1.8 and Nav1.9 sodium and calcium currents.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Hainantoxin-III 12 Publications
Short name:
HnTx-III2 Publications
Alternative name(s):
Hainantoxin-3
Mu-theraphotoxin-Hhn2a
Short name:
Mu-TRTX-Hhn2a
Peptide F7-18.76
OrganismiHaplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Taxonomic identifieri209901 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000339. mu-theraphotoxin-Hhn2a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000040050022 – 482 PublicationsAdd BLAST27
PeptideiPRO_000040050149 – 81Hainantoxin-III 12 PublicationsAdd BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 652 PublicationsImported
Disulfide bondi57 ↔ 702 PublicationsImported
Disulfide bondi64 ↔ 772 PublicationsImported
Modified residuei81Leucine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.2 Publications

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni59 – 62Combined sources4
Beta strandi68 – 70Combined sources3
Beta strandi72 – 74Combined sources3
Beta strandi76 – 78Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JTBNMR-A49-81[»]
SMRiD2Y1X9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD2Y1X9.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.1 Publication

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Hntx-3 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2Y1X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKASMFLALA GLVLLFVVGY ASESEEKEFP RELLSKIFAV DDFKGEERGC
60 70 80
KGFGDSCTPG KNECCPNYAC SSKHKWCKVY LGK
Length:83
Mass (Da):9,195
Last modified:March 2, 2010 - v1
Checksum:iAEE1DBFDFB29B622
GO

Mass spectrometryi

D2Y1X9: Molecular mass is 3607.6 Da from positions 49 - 81. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU292856 mRNA. Translation: ADB56672.1.
GU293061 Genomic DNA. Translation: ADB56877.1.

Similar proteinsi

Entry informationi

Entry nameiH3A01_HAPHA
AccessioniPrimary (citable) accession number: D2Y1X9
Secondary accession number(s): P83464
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 2, 2010
Last modified: October 25, 2017
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families