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Protein

Mu-theraphotoxin-Hhn2b 1

Gene
N/A
Organism
Haplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is a depressant toxin. Blocks the SCN2A/SCN1B (Nav1.2/Beta1) sodium channel receptor (IC50 is 68 +-6 µM) and the insect sodium channel para/tipE (IC50 is 4.3 +-0.3 µM), without altering the activation or inactivation kinetics.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-theraphotoxin-Hhn2b 1
Short name:
Mu-TRTX-Hhn2b
Alternative name(s):
Hainantoxin-1
Hainantoxin-I
Short name:
HnTx-I
Peptide F5-19.03
OrganismiHaplopelma hainanum (Chinese bird spider) (Selenocosmia hainana)
Taxonomic identifieri209901 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000338. mu-theraphotoxin-Hhn2b.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000040049522 – 482 PublicationsAdd BLAST27
PeptideiPRO_000004500449 – 81Mu-theraphotoxin-Hhn2b 1Add BLAST33

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi50 ↔ 651 Publication
Disulfide bondi57 ↔ 701 Publication
Disulfide bondi64 ↔ 771 Publication
Modified residuei81Leucine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

183
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni59 – 62Combined sources4
Beta strandi68 – 70Combined sources3
Beta strandi72 – 79Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NIXNMR-A49-81[»]
SMRiD2Y1X6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiD2Y1X6.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Hntx-1 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

D2Y1X6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKASMFLALA GLVLLFVVCY ASESEEKEFP RELISKIFAV DDFKGEEREC
60 70 80
KGFGKSCVPG KNECCSGYAC NSRDKWCKVL LGK
Length:83
Mass (Da):9,240
Last modified:March 2, 2010 - v1
Checksum:iA72E5B88B0E04922
GO

Mass spectrometryi

Molecular mass is 3608.02 Da from positions 49 - 81. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU292853 mRNA. Translation: ADB56669.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GU292853 mRNA. Translation: ADB56669.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NIXNMR-A49-81[»]
SMRiD2Y1X6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000338. mu-theraphotoxin-Hhn2b.

Miscellaneous databases

EvolutionaryTraceiD2Y1X6.

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiH1A01_HAPHA
AccessioniPrimary (citable) accession number: D2Y1X6
Secondary accession number(s): P83591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 2, 2010
Last modified: November 2, 2016
This is version 22 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.